The Science and Practice of Managing Forests in Cities: Introduction to the Special Issue

The following special issue includes a practitioner note that provides context about Forested Natural Areas in cities and introduces eight themes that are explored in the 25 practitioner case studies. This publication is the product of a fruitful workshop that brought together leaders in the field of Forested Natural Areas management. The case studies document both widespread approaches that are being used in cities across the U.S., as well as innovation that is taking place in individual cities. The goals of both the workshop and this publication are to raise national awareness of urban forested natural areas, improve their management, and contribute to a community of practice

Realizing the Potential of Urban Forests: Forests in Cities Workshop Themes and City Case Study Descriptions

Like all forests, forests in cities require resources, science, management, protection, and programming in order to achieve desired conditions. Yet, achieving the desired condition in an urban environment may require new approaches that account for the dynamic and complex nature of the urban setting. These decisions and actions are occurring at the city, park, or site scale. Unlike national parks, or wilderness areas that have a national or state budget, staffing structures, and regulations; forests in cities are regulated and managed primarily at the local municipal scale. This also makes the approaches to forest management planning and policy regulations tailored to individual city governance structures, despite many similar challenges arising at the national level. As part of our Forest in Cities Workshop, we partnered with 12 cities across the United States to create case studies around common themes related to achieving healthy forests as a part of sustainable and healthy cities. Our goal was to provide examples of work on the ground but also provide a lens upon which the work of individual cities could be contextualized as part of common themes and solutions that could be applied broadly. Below we describe the themes of the workshop and the case studies developed by the cities that are included in this special issue

Data Mining Techniques to Study Therapy Success with Autistic Children

Autism spectrum disorder has become one of the most prevalent developmental disorders, characterized by a wide variety of symptoms. Many children need extensive therapy for years to improve their behavior and facilitate integration in society. However, few systematic evaluations are done on a large scale that can provide insights into how, where, and how therapy has an impact. We describe how data mining techniques can be used to provide insights into behavioral therapy as well as its effect on participants. To this end, we are developing a digital library of coded video segments that contains data on appropriate and inappropriate behavior of autistic children in different social settings during different stages of therapy and. In general, we found that therapy increased appropriate behavior and decreased inappropriate behavior. Decision trees and association rules provided more detailed insights for high and low levels of appropriate and inappropriate behavior. We found that a child\u27s interaction with a parent or therapist led to especially high levels of appropriate behavior and behavior is most predictable while therapy is in progress

GoFish: A versatile nested PCR strategy for environmental DNA assays for marine vertebrates

Here we describe GoFish, a strategy for single-species environmental DNA (eDNA) presence/absence assays using nested PCR. The assays amplify a mitochondrial 12S rDNA segment with vertebrate metabarcoding primers, followed by nested PCR with M13-tailed, species-specific primers. Sanger sequencing confirms positives detected by gel electrophoresis. We first obtained 12S sequences from 77 fish specimens for 36 northwestern Atlantic taxa not well documented in GenBank. Using these and existing 12S records, we designed GoFish assays for 11 bony fish species common in the lower Hudson River estuary and tested seasonal abundance and habitat preference at two sites. Additional assays detected nine cartilaginous fish species and a marine mammal, bottlenose dolphin, in southern New York Bight. GoFish sensitivity was equivalent to Illumina MiSeq metabarcoding. Unlike quantitative PCR (qPCR), GoFish does not require tissues of target and related species for assay development and a basic thermal cycler is sufficient. Unlike Illumina metabarcoding, indexing and batching samples are unnecessary and advanced bioinformatics expertise is not needed. From water collection to Sanger sequencing results, the assay can be carried out in three days. The main limitations to this approach, which employs metabarcoding primers, are the same as for metabarcoding, namely, inability to distinguish species with shared target sequences and inconsistent amplification of rarer eDNA. In addition, the performance of the 20 assays reported here as compared to other single-species eDNA assays is not known. This approach will be a useful addition to current eDNA methods when analyzing presence/absence of known species, when turnaround time is important, and in educational settings

Non-verbal Communication with Autistic Children Using Digital Libraries

Autism spectrum disorder (ASD) has become one of the most prevalent mental disorders over the last few years and its prevalence is still growing. The disorder is characterized by a wide variety of symptoms such as lack of social behavior, extreme withdrawal, and problems communicating. Because of the diversity in symptoms and the wide variety in severity for those, each autistic child has different needs and requires individualized therapy. This leads to long waiting lists for therapy

\u3ci\u3eParamecium bursaria\u3c/i\u3e Chlorella Virus 1 Encodes a Polyamine Acetyltransferase

Background: PBCV-1 gene a654l encodes a protein with sequence similarity to GCN5 histone acetyltransferases. Results: A crystal structure of A654L bound to coenzyme A reveals how A654L acetylates polyamines, not histone lysines. Conclusion: A654L functions as a polyamine acetyltransferase. Significance: As the first viral polyamine acetyltransferase, A654L has a possible role in host polyamine catabolism in viral replication. Paramecium bursaria chlorella virus 1 (PBCV-1), a large DNA virus that infects green algae, encodes a histone H3 lysine 27-specific methyltransferase that functions in global transcriptional silencing of the host. PBCV-1 has another gene a654l that encodes a protein with sequence similarity to the GCN5 family histone acetyltransferases. In this study, we report a 1.5A˚ crystal structure of PBCV-1 A654L in a complex with coenzyme A. The structure reveals a unique feature of A654L that precludes its acetylation of histone peptide substrates. We demonstrate that A654L, hence named viral polyamine acetyltransferase (vPAT), acetylates polyamines such as putrescine, spermidine, cadaverine, and homospermidine present in both PBCV-1 and its host through a reaction dependent upon a conserved glutamate 27. Our study suggests that as the first virally encoded polyamine acetyltransferase, vPAT plays a possible key role in the regulation of polyamine catabolism in the host during viral replication. Includes Supplemental Material

\u3ci\u3eParamecium bursaria\u3c/i\u3e Chlorella Virus 1 Encodes a Polyamine Acetyltransferase

Background: PBCV-1 gene a654l encodes a protein with sequence similarity to GCN5 histone acetyltransferases. Results: A crystal structure of A654L bound to coenzyme A reveals how A654L acetylates polyamines, not histone lysines. Conclusion: A654L functions as a polyamine acetyltransferase. Significance: As the first viral polyamine acetyltransferase, A654L has a possible role in host polyamine catabolism in viral replication. Paramecium bursaria chlorella virus 1 (PBCV-1), a large DNA virus that infects green algae, encodes a histone H3 lysine 27-specific methyltransferase that functions in global transcriptional silencing of the host. PBCV-1 has another gene a654l that encodes a protein with sequence similarity to the GCN5 family histone acetyltransferases. In this study, we report a 1.5A˚ crystal structure of PBCV-1 A654L in a complex with coenzyme A. The structure reveals a unique feature of A654L that precludes its acetylation of histone peptide substrates. We demonstrate that A654L, hence named viral polyamine acetyltransferase (vPAT), acetylates polyamines such as putrescine, spermidine, cadaverine, and homospermidine present in both PBCV-1 and its host through a reaction dependent upon a conserved glutamate 27. Our study suggests that as the first virally encoded polyamine acetyltransferase, vPAT plays a possible key role in the regulation of polyamine catabolism in the host during viral replication. Includes Supplemental Material

antiSMASH 6.0: improving cluster detection and comparison capabilities

Microbial Biotechnolog

Fusion cross section for the system <SUP>6</SUP>Li+<SUP>28</SUP>Si at E~36 MeV

The fusion cross section for the system 6Li+28Si has been measured at E~36 MeV. Combining this with the data available at lower energies, the nucleus-nucleus real potentials have been determined for a range of interaction distances

Brief Report: Theatre as Therapy for Children with Autism Spectrum Disorder

The pilot investigation evaluated a theatrical intervention program, Social Emotional NeuroScience Endocrinology (SENSE) Theatre, designed to improve socioemotional functioning and reduce stress in children with autism spectrum disorder (ASD). Eight children with ASD were paired with typically developing peers that served as expert models. Neuropsychological, biological (cortisol and oxytocin), and behavioral measures were assessed in a pretest–posttest design. The intervention was embedded in a full musical theatrical production. Participants showed some improvement in face identification and theory of mind skills. The intervention shows potential promise in improving the socioemotional functioning in children with ASD through the utilization of peers, video and behavioral modeling, and a community-based theatrical setting