23 research outputs found

    Downregulation of a UDP-Arabinomutase Gene in Switchgrass (Panicum virgatum L.) Results in Increased Cell Wall Lignin While Reducing Arabinose-Glycans

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    Background: Switchgrass (Panicum virgatum L.) is a C4 perennial prairie grass and a dedicated feedstock for lignocellulosic biofuels. Saccharification and biofuel yields are inhibited by the plant cell wall’s natural recalcitrance against enzymatic degradation. Plant hemicellulose polysaccharides such as arabinoxylans structurally support and cross-link other cell wall polymers. Grasses predominately have Type II cell walls that are abundant in arabinoxylan, which comprise nearly 25% of aboveground biomass. A primary component of arabinoxylan synthesis is uridine diphosphate (UDP) linked to arabinofuranose (Araf). A family of UDP-arabinopyranose mutase (UAM)/reversible glycosylated polypeptides catalyze the interconversion between UDP-arabinopyranose (UDP-Arap) and UDP-Araf. Results: The expression of a switchgrass arabinoxylan biosynthesis pathway gene, PvUAM1, was decreased via RNAi to investigate its role in cell wall recalcitrance in the feedstock. PvUAM1 encodes a switchgrass homolog of UDP-arabinose mutase, which converts UDP-Arap to UDP-Araf. Southern blot analysis revealed each transgenic line contained between one to at least seven T-DNA insertions, resulting in some cases, a 95% reduction of native PvUAM1 transcript in stem internodes. Transgenic plants had increased pigmentation in vascular tissues at nodes, but were otherwise similar in morphology to the non-transgenic control. Cell wall-associated arabinose was decreased in leaves and stems by over 50%, but there was an increase in cellulose. In addition, there was a commensurate change in arabinose side chain extension. Cell wall lignin composition was altered with a concurrent increase in lignin content and transcript abundance of lignin biosynthetic genes in mature tillers. Enzymatic saccharification efficiency was unchanged in the transgenic plants relative to the control. Conclusion: Plants with attenuated PvUAM1 transcript had increased cellulose and lignin in cell walls. A decrease in cell wall-associated arabinose was expected, which was likely caused by fewer Araf residues in the arabinoxylan. The decrease in arabinoxylan may cause a compensation response to maintain cell wall integrity by increasing cellulose and lignin biosynthesis. In cases in which increased lignin is desired, e.g., feedstocks for carbon fiber production, downregulated UAM1 coupled with altered expression of other arabinoxylan biosynthesis genes might result in even higher production of lignin in biomass

    The motivations of psychotherapists: an in‐depth survey

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    Background Examining the motivations of psychotherapists has not been a popular topic of systematic research. Knowing why people want to become therapists is clearly important because this factor will inevitably impact on therapeutic outcomes. The absence of research‐led knowledge on therapists′ motivations allows this key issue to remain a relatively unattended focus within reflective practice and personal development. Aim To collect data about therapists′ motivations from a large number of practitioners so that core findings could be generalised to the wider profession. Method A total of 540 psychotherapists completed an online survey with significant numbers offering supporting qualitative data. Quantitative data were analysed using descriptive and inferential statistics. Qualitative data were subject to thematic and textual analysis. Results A large majority of therapists believe that their career choice was influenced by unconscious motivations, that their motivations are likely to change over time and that their own psychic wounds can contribute to effective therapy. Conclusion Psychotherapists are clearly prepared to reflect, in depth, on why they have been drawn to the profession. The fact that an awareness of therapists′ motivations may be variable that they may change over time and can be linked to personal vulnerability suggests that the topic should be an integral part of practitioners′ ongoing personal development and a discrete focus in formal training programmes
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