Analysis of Sertoli Cell-Secreted Proteins by Two-Dimensional Gel Electrophoresis

The [35S]methionine-labeled proteins secreted from cultured Sertoli cells were analyzed by two-dimensional gel electrophoresis and fluorography. Major polypeptides which were resolved by this procedure were designated by number and further analyzed. Many of these major polypeptides appeared as a series of spots which corresponded to charge isomers. Two of these polypeptides (5 and 6) were shown to be acidic, glycosylated and to comprise the subunits of a dimeric protein of molecular weight 70,000. Some of the polypeptides (4a and 5a) were shown to be secreted from testicular peritubular cells which contaminated the Sertoli cell cultures. However, many of the polypeptides (1,2,3,4,5,5b and 6) were specifically secreted from the Sertoli cells. The fluorogram of the secreted polypeptides obtained from cultured Sertoli cells from 20- or 60-day-old rats were similar to each other but differed from the pattern of polypeptides which were secreted from cultures of Sertoli cells from 10-day-old rats. Polypeptide 3 was identified by immunoprecipitation as testicular transferrin and the synthesis of polypeptide 3 was stimulated when the Sertoli cells were cultured in the presence of follicle-stimulating hormone (FSH), insulin, testosterone and retinol

New genus Malagiella.

86 p. : ill. (some col.), col. maps ; 26 cm.A new oonopid genus endemic to Madagascar, Malagiella, is proposed and its 10 constituent species newly described and illustrated: M. ambalavo ([male]), M. andringitra ([male, female]), M. fisheri ([m, f]), M. goodmani ([f]), M. nikina ([f]), M. ranavalona ([f]), M. ranomafana ([m, f]), M. toliara ([m, f]), M. valterova ([m, f]), and M. vohiparara ([m, f]). Malagiella is most similar to the Asian genus Camptoscaphiella in both somatic and genitalic features, but differs in some important characters. Unlike Camptoscaphiella, the Malagiella male has a palpal bulb completely fused with the tarsus and a sternum bearing stiff bristles; the female has a round copulatory opening and bifid 4th claws. The species may be recognized by the genitalia. Male palpi are fairly uniform, but differ in segment proportion and details of the embolus complex, which consists of a broad dorsal embolus connected to a laminar ventral prong. Female genitalia are more variable, especially in the curvature of the receptaculum, which spans the range from nearly straight to widely sinuous and occurs in mirror-image versions having opposite coiling. These torsional variants, with females having either left- or right-handed receptacula, probably occur in all species, although they are not evident in species with straight receptacula and (obviously) unknown in species represented by singleton female specimens. Malagiella species are very diverse somatically, ranging from dark, big, and large-eyed species to pale, small, and reduced-eyed ones. Our preliminary clustering uses mostly these somatic features, with some genitalic support, to identify three species groups: ranomafana, vohiparara, and toliara. Other genitalic characters that suggest different groupings are discussed

New genus Molotra

69 p. : ill. (some col.), col. map ; 26 cm.A new oonopid genus endemic to Madagascar, Molotra, is proposed and its six included species are newly described and illustrated: M. molotra, the type species, M. ninae, M. katarinae, M. suzannae, M. tsingy, and M. milloti. Molotra is defined on the basis of its unusual genitalia. The male has the palpal bulb terminating in broad, liplike lobes, unlike any previously described in Oonopidae. The female is equally unusual in lacking an apparent receptaculum, although the genitalia otherwise resemble those of silhouettelloid genera. The species of Molotra form three distinct groups based on somatic and genitalic characters. The M. molotra group includes four large, strongly spined species. The remaining two are morphologically distinct and known only from males. M. tsingy is a small species with unusual palpi that are complex, contorted, and with a resting position along the sternocoxal boundary, instead of an anterior sternal cavity. M. milloti is a very bristly species that differs somatically from other Molotra but has genitalia similar to the M. molotra group. Molotra is known only from Madagascar where the three species groups are strongly disjunct. The M. molotra group occurs in higher elevation forests (1000-1300 m) in northeastern Madagascar, M. tsingy is from low elevation karst (150 m) of the southwest, whereas M. milloti is from a coastal locality in the northwest

African Orsolobidae

14 p. : ill., map ; 26 cm.Includes bibliographical references (p. 14)."The first African representatives of the austral spider family Orsolobidae are described. Two new genera are established: Afrilobus, containing three new species (A. capensis and A. australis from the Cape Province of South Africa, and A. jocquei from Malawi), and Azanialobus, conatining one new species, A. lawrencei, from Transvaal, South Africa"--P. [1]

The Southeast Asian genus Stedocys Ono, 1995 (Araneae: Scytodidae): First descriptions of female genitalia and a new species from China

The genus Stedocys was known only from males. Here we describe the first females of the genus. Stedocys genitalia are atypical for Scytodidae. Males are distinguished by having the papal tarsus subequal or smaller than the tegulum, not prolonged apically, without prolateral blunt macrosetae; the tegulum long, inserted apically on the tarsus; embolus slightly shorter than bulb, aciculate distally, and females by lacking fovea or positioning ridges below the epigastric furrow, and by having an anterior epigynal pouch. In this contribution, we describe the female and redescribe the male of Stedocys leopoldi (Giltay, 1935), and describe male and female of Stedocys pagodas new species from China (Yunnan). The lack of a projection on the male palpal tarsus suggests that Stedocys is the sister group of all other genera of Scytodidae.Fil: Labarque, Facundo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; ArgentinaFil: Grismado, Cristian José. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; ArgentinaFil: Ramírez Martínez, José de Jesús. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; ArgentinaFil: Yan, Hengmei. Hunan Normal University; ChinaFil: Griswold, Charles. California Academy Of Sciences.; Estados Unido