Intestinal Bacteria Composition and Translocation of Bacteria in Inflammatory Bowel Disease.

Live commensal intestinal bacteria are present in the peripheral blood where they can induce inflammation.To evaluate the intestinal bacteria composition and translocation of bacteria in IBD.Both blood and tissue biopsy samples were collected from adult patients with active/inactive Crohn's disease (CD), active/inactive ulcerative colitis (UC) and healthy individuals. Most of the patients were newly diagnosed and none of them received antibiotics. Using a reverse transcription-quantitative real-time PCR (RT-qPCR) method, we determined the composition of microbiota. NOD2/CARD15 genotyping was also studied.Total bacterial DNA concentration was increased in tissue and blood samples of IBD patients compared to healthy controls. Furthermore, the active IBD cases had higher total bacterial DNA concentration levels compared to the inactive cases. Three species characterized dysbiosis in IBD, namely an increase of Bacteroides spp in active and inactive IBD samples, and a decrease in Clostridium leptum group (IV), and Faecalibacterium prausnitzi in both active and inactive IBD patients. No significant association between bacterial translocation and NOD2/CARD15 mutations was found.The composition of the microbiota in IBD patients differs from that of healthy controls. The high rate of bacterial DNA in the blood samples indicates translocation in inflammatory bowel disease

Total bacteria DNA (ng/μl) in tissue and blood microbiota.

<p>Total bacteria DNA (ng/μl) in tissue and blood microbiota.</p

Total bacterial DNA (ng/μl) per patient and healthy controls.

<p>Samples were grouped according to their pathological stage: active NC and UC, and inactive NC and UC.</p

Clinical and analytical characteristics of patients included in the study.

<p>Clinical and analytical characteristics of patients included in the study.</p

Primers targeting 16S rRNA gene used for determination of microbiota composition by real time PCR.

<p>Primers targeting 16S rRNA gene used for determination of microbiota composition by real time PCR.</p

Quantification of dominant bacterial in biopsies.

<p>The bars represent the percentage of total bacterial counts in active and inactive CD, in active and inactive UC cases and in heathy samples. * <i>P</i> < 0.05; ** P < 0.01; *** <i>P</i> < 0.001.</p

Quantification of bacterial in biopsies and blood microbiota.

<p>Quantification of bacterial in biopsies and blood microbiota.</p