Flow Dynamics And Plasma Heating Of Spheromaks In SSX

We report several new experimental results related to flow dynamics and heating from single dipole-trapped spheromaks and spheromak merging studies at SSX. Single spheromaks (stabilized with a pair of external coils, see Brown, Phys. Plasmas 13 102503 (2006)) and merged FRC-like configurations (see Brown, Phys. Plasmas 13, 056503 (2006)) are trapped in our prolate (R = 0.2 m, L = 0.6 m) copper flux conserver. Local spheromak flow is studied with two Mach probes (r(1) = rho(i) ) calibrated by time-of-flight with a fast set of magnetic probes at the edge of the device. Both Mach probes feature six ion collectors housed in a boron nitride sheath. The larger Mach probe will ultimately be used in the MST reversed field pinch. Line averaged flow is measured by ion Doppler spectroscopy (IDS) at the midplane. The SSX IDS instrument measures with 1 mu s or better time resolution the width and Doppler shift of the C-III impurity (H plasma) 229.7 nm line to determine the temperature and line-averaged flow velocity (see Cothran, RSI 77, 063504 (2006)). We find axial flows up to 100 km/s during formation of the dipole trapped spheromak. Flow returns at the wall to form a large vortex. Recent high-resolution IDS velocity measurements during spheromak merging show bi-directional outflow jets at +/- 40 km/s (nearly the Alfven speed). We also measure T-i \u3e= 80 eV and T-e \u3e= 20 eV during spheromak merging events after all plasma facing surfaces are cleaned with helium glow discharge conditioning. Transient electron heating is inferred from bursts on a four-channel soft x-ray array. The spheromaks are also characterized by a suite of magnetic probe arrays for magnetic structure B(r,t), and interferometry for n(e) . Finally, we are designing a new oblate, trapezoidal flux conserver for FRC studies. Equilibrium and dynamical simulations suggest that a tilt-stable, oblate FRC can be formed by spheromak merging in the new flux conserver

Upregulation of FOXM1 induces genomic instability in human epidermal keratinocytes

<p>Abstract</p> <p>Background</p> <p>The human cell cycle transcription factor FOXM1 is known to play a key role in regulating timely mitotic progression and accurate chromosomal segregation during cell division. Deregulation of FOXM1 has been linked to a majority of human cancers. We previously showed that FOXM1 was upregulated in basal cell carcinoma and recently reported that upregulation of FOXM1 precedes malignancy in a number of solid human cancer types including oral, oesophagus, lung, breast, kidney, bladder and uterus. This indicates that upregulation of FOXM1 may be an early molecular signal required for aberrant cell cycle and cancer initiation.</p> <p>Results</p> <p>The present study investigated the putative early mechanism of UVB and FOXM1 in skin cancer initiation. We have demonstrated that UVB dose-dependently increased FOXM1 protein levels through protein stabilisation and accumulation rather than de novo mRNA expression in human epidermal keratinocytes. FOXM1 upregulation in primary human keratinocytes triggered pro-apoptotic/DNA-damage checkpoint response genes such as p21, p38 MAPK, p53 and PARP, however, without causing significant cell cycle arrest or cell death. Using a high-resolution Affymetrix genome-wide single nucleotide polymorphism (SNP) mapping technique, we provided the evidence that FOXM1 upregulation in epidermal keratinocytes is sufficient to induce genomic instability, in the form of loss of heterozygosity (LOH) and copy number variations (CNV). FOXM1-induced genomic instability was significantly enhanced and accumulated with increasing cell passage and this instability was increased even further upon exposure to UVB resulting in whole chromosomal gain (7p21.3-7q36.3) and segmental LOH (6q25.1-6q25.3).</p> <p>Conclusion</p> <p>We hypothesise that prolonged and repeated UVB exposure selects for skin cells bearing stable FOXM1 protein causes aberrant cell cycle checkpoint thereby allowing ectopic cell cycle entry and subsequent genomic instability. The aberrant upregulation of FOXM1 serves as a 'first hit' where cells acquire genomic instability which in turn predisposes cells to a 'second hit' whereby DNA-damage checkpoint response (eg. p53 or p16) is abolished to allow damaged cells to proliferate and accumulate genetic aberrations/mutations required for cancer initiation.</p

Spectroscopic Measurements Of Temperature And Plasma Impurity Concentration During Magnetic Reconnection At The Swarthmore Spheromak Experiment

Electron temperature measurements during counterhelicity spheromak merging studies at the Swarthmore Spheromak Experiment (SSX) [M. R. Brown, Phys. Plasmas 6, 1717 (1999)] are presented. VUV monochromator measurements of impurity emission lines are compared with model spectra produced by the non-LTE excitation kinematics code PRISMSPECT [J. J. MacFarlane et al., in Proceedings of the Third Conference on Inertial Fusion Science and Applications (2004)] to yield the electron temperature in the plasma with 1 mu s time resolution. Average T(e) is seen to increase from 12 to 19 eV during spheromak merging. Average C III ion temperature, measured with a new ion Doppler spectrometer (IDS) [C. D. Cothran et al., Rev. Sci. Instrum. 77, 063504 (2006)], likewise rises during spheromak merging, peaking at similar to 22 eV, but a similar increase in T(i) is seen during single spheromak discharges with no merging. The VUV emission line measurements are also used to constrain the concentrations of various impurities in the SSX plasma, which are dominated by carbon, but include some oxygen and nitrogen. A burst of soft x-ray emission is seen during reconnection with a new four-channel detector (SXR). There is evidence for spectral changes in the soft x-ray emission as reconnection progresses, although our single-temperature equilibrium spectral models are not able to provide adequate fits to all the SXR data. (C) 2009 American Institute of Physics. [DOI: 10.1063/1.3099603

3D Reconnection And Flow Dynamics In The SSX Experiment

Several new experimental results are reported from plasma merging studies at the Swarthmore Spheromak Experiment (SSX) with relevance to collisionless three-dimensional magnetic reconnection in laboratory and space plasmas. First, recent high-resolution velocity measurements of impurity ions using ion Doppler spectroscopy (IDS) show bi-directional outflow jets at 40 km/s (nearly the Alfven speed). The SSX IDS instrument measures with 1 mu s or better time resolution the width and Doppler shift of the C-III impurity (H plasma) 229.7 nm line to determine the temperature and line-averaged flow velocity during spheromak merging events. High flow speeds are corroborated using an in situ Mach probe. Second, ion heating to nearly 10(6) K is observed after reconnection events in a low-density kinetic regime. Transient electron heating is inferred from bursts on a 4-channel soft x-ray array as well as vacuum ultraviolet spectroscopy. Third, the out-of-plane magnetic field and the in-plane Lorentz force in a reconnection volume both show a quadrupolar structure at the ion inertial scale (c/omega(pi)). Time resolved vector magnetic field measurements on a 3D lattice B(r, t)) enables this measurement. Earlier work at SSX has shown that fori-nation of three-dimensional structure at the ion inertial scale is temporally and spatially correlated with the observation of superthermal, super-Alfvenic ions accelerated along the X-line normal to the local 2D plane of reconnection. Each of these measurements will be related to and compared with similar observations in a solar or space context

The K2 Mission: Characterization and Early results

The K2 mission will make use of the Kepler spacecraft and its assets to expand upon Kepler's groundbreaking discoveries in the fields of exoplanets and astrophysics through new and exciting observations. K2 will use an innovative way of operating the spacecraft to observe target fields along the ecliptic for the next 2-3 years. Early science commissioning observations have shown an estimated photometric precision near 400 ppm in a single 30 minute observation, and a 6-hour photometric precision of 80 ppm (both at V=12). The K2 mission offers long-term, simultaneous optical observation of thousands of objects at a precision far better than is achievable from ground-based telescopes. Ecliptic fields will be observed for approximately 75-days enabling a unique exoplanet survey which fills the gaps in duration and sensitivity between the Kepler and TESS missions, and offers pre-launch exoplanet target identification for JWST transit spectroscopy. Astrophysics observations with K2 will include studies of young open clusters, bright stars, galaxies, supernovae, and asteroseismology.Comment: 25 pages, 11 figures, Accepted to PAS

Application of a correlation correction factor in a microarray cross-platform reproducibility study

Background Recent research examining cross-platform correlation of gene expression intensities has yielded mixed results. In this study, we demonstrate use of a correction factor for estimating cross-platform correlations. Results In this paper, three technical replicate microarrays were hybridized to each of three platforms. The three platforms were then analyzed to assess both intra- and cross-platform reproducibility. We present various methods for examining intra-platform reproducibility. We also examine cross-platform reproducibility using Pearson\u27s correlation. Additionally, we previously developed a correction factor for Pearson\u27s correlation which is applicable when X and Y are measured with error. Herein we demonstrate that correcting for measurement error by estimating the disattenuated correlation substantially improves cross-platform correlations. Conclusion When estimating cross-platform correlation, it is essential to thoroughly evaluate intra-platform reproducibility as a first step. In addition, since measurement error is present in microarray gene expression data, methods to correct for attenuation are useful in decreasing the bias in cross-platform correlation estimates

The diagnosis of inherited metabolic diseases by microarray gene expression profiling

<p>Abstract</p> <p>Background</p> <p>Inherited metabolic diseases (IMDs) comprise a diverse group of generally progressive genetic metabolic disorders of variable clinical presentations and severity. We have undertaken a study using microarray gene expression profiling of cultured fibroblasts to investigate 68 patients with a broad range of suspected metabolic disorders, including defects of lysosomal, mitochondrial, peroxisomal, fatty acid, carbohydrate, amino acid, molybdenum cofactor, and purine and pyrimidine metabolism. We aimed to define gene expression signatures characteristic of defective metabolic pathways.</p> <p>Methods</p> <p>Total mRNA extracted from cultured fibroblast cell lines was hybridized to Affymetrix U133 Plus 2.0 arrays. Expression data was analyzed for the presence of a gene expression signature characteristic of an inherited metabolic disorder and for genes expressing significantly decreased levels of mRNA.</p> <p>Results</p> <p>No characteristic signatures were found. However, in 16% of cases, disease-associated nonsense and frameshift mutations generating premature termination codons resulted in significantly decreased mRNA expression of the defective gene. The microarray assay detected these changes with high sensitivity and specificity.</p> <p>Conclusion</p> <p>In patients with a suspected familial metabolic disorder where initial screening tests have proven uninformative, microarray gene expression profiling may contribute significantly to the identification of the genetic defect, shortcutting the diagnostic cascade.</p

Burst and persistent emission properties during the recent active episode of the anomalous x-ray pulsar 1E 1841-045

Copyright American Astronomical SocietyThe Swift/Burst Alert Telescope detected the first burst from 1E 1841-045 in 2010 May with intermittent burst activity recorded through at least 2011 July. Here we present Swift and Fermi/Gamma-ray Burst Monitor observations of this burst activity and search for correlated changes to the persistent X-ray emission of the source. The T-90 durations of the bursts range between 18 and 140 ms, comparable to other magnetar burst durations, while the energy released in each burst ranges between (0.8-25) x 10(38) erg, which is on the low side of soft gamma repeater bursts. We find that the bursting activity did not have a significant effect on the persistent flux level of the source. We argue that the mechanism leading to this sporadic burst activity in 1E 1841-045 might not involve large-scale restructuring (either crustal or magnetospheric) as seen in other magnetar sources.Peer reviewedFinal Accepted Versio

Kepler-93b: A Terrestrial World Measured to within 120 km, and a Test Case for a New Spitzer Observing Mode

We present the characterization of the Kepler-93 exoplanetary system, based on three years of photometry gathered by the Kepler spacecraft. The duration and cadence of the Kepler observations, in tandem with the brightness of the star, enable unusually precise constraints on both the planet and its host. We conduct an asteroseismic analysis of the Kepler photometry and conclude that the star has an average density of 1.652+/-0.006 g/cm^3. Its mass of 0.911+/-0.033 M_Sun renders it one of the lowest-mass subjects of asteroseismic study. An analysis of the transit signature produced by the planet Kepler-93b, which appears with a period of 4.72673978+/-9.7x10^-7 days, returns a consistent but less precise measurement of the stellar density, 1.72+0.02-0.28 g/cm^3. The agreement of these two values lends credence to the planetary interpretation of the transit signal. The achromatic transit depth, as compared between Kepler and the Spitzer Space Telescope, supports the same conclusion. We observed seven transits of Kepler-93b with Spitzer, three of which we conducted in a new observing mode. The pointing strategy we employed to gather this subset of observations halved our uncertainty on the transit radius ratio R_p/R_star. We find, after folding together the stellar radius measurement of 0.919+/-0.011 R_Sun with the transit depth, a best-fit value for the planetary radius of 1.481+/-0.019 R_Earth. The uncertainty of 120 km on our measurement of the planet's size currently renders it one of the most precisely measured planetary radii outside of the Solar System. Together with the radius, the planetary mass of 3.8+/-1.5 M_Earth corresponds to a rocky density of 6.3+/-2.6 g/cm^3. After applying a prior on the plausible maximum densities of similarly-sized worlds between 1--1.5 R_Earth, we find that Kepler-93b possesses an average density within this group.Comment: 20 pages, 9 figures, accepted for publication in Ap