The Miliuseae revisited : phylogenetic, taxonomic, and palynological studies in a major clade of Annonaceae

This thesis presented eight studies in the field of systematic botany, with a focus on the molecular phylogenetics, taxonomy, and pollen morphology of the tribe Miliuseae (formerly known as the miliusoid clade) of the pantropical flowering plant family Annonaceae. The general aims were: (1) to investigate intertribal relationships of the tribe Miliuseae, (2) to clarify generic delimitations by investigating the intergeneric relationships within the tribe Miliuseae, and (3) to understand patterns of character evolution within the tribe Miliuseae. To achieve these aims, a molecular phylogeny of a subclade of the subfamily Malmeoideae consisting of the tribes Maasieae, Malmeeae, Fenerivieae, Dendroking- stonieae (sampled for the first time), Monocarpieae, and Miliuseae was reconstructed using a much expanded taxon and chloroplast DNA marker sampling in comparison to previous studies. In addition, detailed taxonomic and/or palynological studies of the monogeneric tribes Dendrokingstonieae and Monocarpieae, and several genera in Miliuseae have been presented. The evolution of selected macromorphological and palynological characters was investigated using ancestral character-state reconstructions. Generic delimitations within Miliuseae, in light of the results of the molecular phylogenetic studies, were discussed, and two new genera, Hubera and Winitia, have been characterized based on macromorphology and pollen morphology.The Royal Thai GovernmentUBL - phd migration 201

An extended phylogeny of pseuduvaria (annonaceae) with descriptions of three new species and a reassessment of the generic status of oreomitra

Three new species of Pseuduvaria (Annonaceae) from Peninsular Thailand and Peninsular Malaysia are described and named as P. fragrans, P. gardneri, and P. glossopetala. A recent molecular phylogenetic study, based on 51 species (including P. fragrans), elucidated the evolutionary relationships within the genus. The present study includes P. gardneri and P. glossopetala in the phylogenetic analyses using maximum likelihood and Bayesian inference methods, based on five chloroplast regions (psbAtrnH spacer, trnLF, matK, rbcL, and atpBrbcL spacer). The new nomenclatural combination Pseuduvaria phuyensis is also validated in accordance with the phylogenetic analyses which show that Craibella phuyensis and Pseuduvaria form a well-supported monophyletic clade. The new species and new nomenclature combination bring the total number of species in Pseuduvaria to 56. The taxonomic status of the enigmatic monotypic genus Oreomitra from New Guinea is furthermore evaluated, and the name shown to be synonymous with Pseuduvaria. © Copyright 2010 by the American Society of Plant Taxonomists.link_to_subscribed_fulltex

Integrative systematics supports the establishment of Winitia, a new genus of Annonaceae (Malmeoideae, Miliuseae) allied to Stelechocarpus and Sageraea

The generic circumscriptions of Stelechocarpus and Sageraea (Annonaceae) are assessed using molecular phylogenetic, macromorphological, and pollen morphological evidence. For molecular phylogenetic analysis the combined seven plastid markers: rbcL exon, trnL intron, trnL-F spacer, matK exon, ndhF exon, psbA-trnH spacer, and ycf1 exon constituting c. 7 kb are used. The results corroborate the recognition of a maximally supported clade as a new genus, Winitia. It is weakly to moderately supported as sister to Stelechocarpus burahol, the type and only species of Stelechocarpus. A clade consisting of Winitia and Stelechocarpus is strongly supported as sister to Sageraea, which is monophyletic with strong support. Winitia consists of two species, one of which (W. expansa) is proposed as a new species endemic to Thailand, whereas one new combination (W. cauliflora) is made. The new genus is primarily characterized by (1) multicolumellar stigmas (= 5 columns per stigma) and (2) pollen grains with a very thin tectum, a more or less columellate/coarsely granular infratectum, and a very distinct basal layer. The macromorphology and pollen morphology of the three genera (Stelechocarpus, Winitia, and Sageraea) are highlighted

Integrative systematics supports the establishment of Winitia, a new genus of Annonaceae (Malmeoideae, Miliuseae) allied to Stelechocarpus and Sageraea

The generic circumscriptions of Stelechocarpus and Sageraea (Annonaceae) are assessed using molecular phylogenetic, macromorphological, and pollen morphological evidence. For molecular phylogenetic analysis the combined seven plastid markers: rbcL exon, trnL intron, trnL-F spacer, matK exon, ndhF exon, psbA-trnH spacer, and ycf1 exon constituting c. 7 kb are used. The results corroborate the recognition of a maximally supported clade as a new genus, Winitia. It is weakly to moderately supported as sister to Stelechocarpus burahol, the type and only species of Stelechocarpus. A clade consisting of Winitia and Stelechocarpus is strongly supported as sister to Sageraea, which is monophyletic with strong support. Winitia consists of two species, one of which (W. expansa) is proposed as a new species endemic to Thailand, whereas one new combination (W. cauliflora) is made. The new genus is primarily characterized by (1) multicolumellar stigmas (= 5 columns per stigma) and (2) pollen grains with a very thin tectum, a more or less columellate/coarsely granular infratectum, and a very distinct basal layer. The macromorphology and pollen morphology of the three genera (Stelechocarpus, Winitia, and Sageraea) are highlighted

Huberantha, a replacement name for Hubera (Annonaceae: Malmeaoideae: Miliuseae)

A replacement generic name, Huberantha Chaowasku, is proposed for the recently described genus Hubera Chaowasku segregated from Polyalthia Blume because the name Hubera has been recommended to be treated as a later homonym of Huberia DC. (Melastomataceae) by the nomenclature committee for vascular plants. Consequently, new combinations of twenty-seven species presently recognised are made under Huberantha

Molecular phylogenetic support for the taxonomic merger of Fitzalania and Meiogyne (Annonaceae): new nomenclatural combinations under the conserved name Meiogyne

Meiogyne (Annonaceae) currently comprises 15 species of trees and shrubs, distributed in India, Southeast Asia, Australasia, Micronesia and Polynesia. Previous molecular phylogenetic studies have shown that the Australian endemic genus Fitzalania (consisting of only two species) is nested within Meiogyne, and preliminary morphological data have indicated that several south Pacific Polyalthia species may be misclassified and also associated with Meiogyne. We use maximum parsimony, maximum likelihood and Bayesian analyses based on seven chloroplast regions (matK, ndhF, ndhF-rpl32, rbcL, rpl32-trnL, trnL-F and ycf1) to reconstruct the most comprehensive phylogeny of Meiogyne available to date, inclusive of 13 Meiogyne species, the two Fitzalania species, as well as four Polyalthia species from Fiji and Tonga. The results show that Fitzalania and the four Polyalthia species are nested within Meiogyne, and that two species, M. cylindrocarpa and M. stenopetala, are not natural as currently defined. Meiogyne cylindrocarpa subsp. trichocarpa and M. stenopetala subsp. insularis are not conspecific with their respective autonymic subspecies and are morphologically distinct. Based on the plastid marker phylogeny, and corroborated by morphological observations, both subspecies are elevated to species rank, and the four Polyalthia species and the two Fitzalania species are transferred to Meiogyne, thereby increasing the number of species in the genus to 24

A decade of uncertainty: Resolving the phyologenetic position of Diclinanona (Annonaceae), inlcuding taxonomic notes and a key to the species

The molecular phylogenetic placement of Diclinanona (Annonaceae) has been debated in the literature for a decade. On the basis of morphological studies the genus was thought to be related to genera now all placed in subfam. Annonoideae. This early hypothesis was supported by the first phylogenetic analyses of Annonaceae. However, more recently a placement in subfam. Malmeoideae was hypothesised based on an analysis of more plastid data, thus contradicting older but also new morphological findings and previous phylogenetic work. The current study uses newly sequenced plastid data for two species of Diclinanona to show that the earlier hypothesised placement was correct and discusses the (little) anatomical and morphological data on Diclinanona that is available in a phylogenetic framework. Furthermore, an online revision of the three species of Diclinanona is presented in order to update the taxonomic knowledge of this genus