ハロアルキル修飾型siRNAおよび分岐型siRNAの合成とその特性評価

博士(農学)岐阜大学(Gifu University

Plant Mediated Green Synthesis of Silver Nanoparticles Using Tecomella undulata Leaf Extract and Their Characterization

Green synthesis of silver nanoparticles (AgNPs) was carried out using aqueous extract of Roheda (Tecomella undulata) at 60 ℃ in orbital shaking incubator. The variation in pH values and colour changes were observed within few minutes during reduction of silver nitrate (0.1 to 1 mM final concentration) in presence of plant extract and the capping of synthesized stable silver nanoparticles. The UV-Vis spectra of reaction mixture at different time intervals were recorded to monitor the formation of nanoparticles. The maximum absorption peak was observed near 430 nm indicating the formation of AgNPs. The particle size distribution was analysed through dynamic light scattering (DLS) and it was found that the peaks at 5.85 nm and 77.48 nm. Polydisperse Index (PDI) of synthesized AgNPs is 0.378 indicating that nanoparticles are stable even after nine months storage and uniform size range. The stability of biogenic silver nanoparticles was monititored through zeta potential measurement. The surface properties and size of the nanoparticles were studied through scanning electron microscopy and atomic force microscopy. The purity of the synthesized nanoparticle was confirmed by electron dispersive X-ray spectroscopy (EDS). Atomic force microscopy images indicated that height of the particle ranges from 3-18 nm. The results of various techniques indicated that these are silver nanoparticles synthesized using leaf extract of Tecomella undulata

Mycosis Fungoides: The great imitator

Introduction: Mycosis fungoides although a cutaneous T-Cell lymphoma can immitate many dermatological disorders. A 60 year old man presented to our hospital with generalized annular plaques, few with verrucous surface. The annular lesions imitated Psoriasis, Tinea, Syphilis and the diagnosis was in dilemma. Histopathology gave the light and path to diagnosis. Case report: A 60 year old man presented with complains of erythematous scaly lesions since 20 year, Lesions were initially flat and erythematous which later became ulcerated, crusted and painful. H/O exacerbation and remission was present. Conclusions: Mycosis Fungoides is a great imitator both clinically and histopathologically. We are presenting a case report of patient with Mycosis Fungoides, Stage IIA

Assessment of clinical VIDA score, lesional and perilesional dermoscopy to evaluate stability of the disease in vitiligo

Introduction: Vitiligo is a complex dermatosis with an uncertain etiology, variable clinical presentations, an unpredictable course and prognosis, and difficult to treat. Surgical intervention is recommended for patients with a stable, nonprogressive vitiligo. Dermoscopy can detect the subtle changes in the pigmentary pattern and aid in the diagnosis of evolving lesions. This study was undertaken to measure the stability of the disease by classifying the patients as clinically stable and active and observing dermatological parameters. Objective: The aim of the study is to evaluate assess the correlation between the clinical and dermoscopic features in stable and active cases of vitiligo. Materials and Methods: Forty patients of vitiligo. Clinical VIDA score and six lesional and perilesional dermoscopic parameters were analyzed. Statistical analysis was done to determine their correlation. Results: Altered pigment network (91.6%) was the most observed dermoscopic feature followed by perifollicular depigmentation (66.7%) in clinically unstable patients and altered pigment network (87.5%) followed by leucotrichia (68.8%) was seen in clinically stable patients. However, vascularity and Koebner's phenomena failed to produce the same. Conclusion: Lesional and perilesional dermoscopic examination together for the above four parameters (pigmentary network, perifollicular changes, perilesional hypopigmentary macules, and leucotrichia) will produce reliable diagnostic approach for differentiating patients as clinically stable or unstable along with clinical VIDA score