Simultaneous estimation of atenolol and chlorthalidone in combine tablet dosage form by absorption ratio method using UV-Vis spectrophotometry

A simple, precise, reproducible, accurate, economical and rapid UV-VIS Spectrophotometric method have been developed and validated for the simultaneous estimation of ATN and CTN in tablet dosage form. This paper describes the absorption ratio method as a quantification parameter. The absorption ratio method (ARM) involves measurement of absorbance of sample solution is measured at 240.0 nm (Isobestic Point) and 251.0 nm (? max of CTN) and based on E 1% 1cm values at these wavelengths two set of equations were framed. The developed method obeys the beers law in the concentration range of 40-80g/mL for ATN and 10-50 g/mL for CTN. The recovery studies shows %RSD for ATN 0.21 and for CTN 1.34 by ARM method. The results of analysis have been validated statistically for accuracy, precision, repeatability, specificity and ruggedness. The method was successfully applied to the determination of these drugs in pharmaceutical dosage form

Portable Fuel Adulteration Detection System

In this paper a portable solution to the problem of adulteration in diesel is proposed. Techniques for checking adulteration in fuel are common. But, the biggest disadvantage of these is that these are lab based and hence require sophisticated testing and analysis in laboratories. The method that is proposed in this paper a portable apparatus that can be directly fitted in vehicles and adulteration can be checked on the go. The method employed uses polarimetry as the basis for sensing adulteration. The apparatus was designed after careful deliberation on various techniques that could be used for checking adulteration in diesel by kerosene. The proposed apparatus uses optical properties of the fuel samples to give the results

DETERMINATION OF ATORVASTATIN CALCIUM IN PHARMACEUTICAL FORMULATIONS BY REVERSE PHASE-HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

A simple, sensitive and reproducible reverse-phase high performance liquid chromatographic (RP-HPLC) method has been developed for the quantitative estimation of Atorvastatin calcium in the pharmaceutical formulations. Chromatographic separation was achieved on a 250 × 4.6 mm, 5μ, Waters symmetry column. The flow rate was 1ml/min and eluent was monitored by absorbance at 246 nm using a mixture of Methanol and Acetonitrile (pH 3.

DETERMINATION OF TELMISARTAN AND FORCED DEGRADATION BEHAVIOR BY RP-HPLC IN TABLET DOSAGE FORM

A simple, rapid, precise, rapid, sensitive and reproducible reverse phase high performance liquid chromatographic (RP-HPLC) method for determination of Telmisartan in tablet dosage form was developed and validated. Chromatographic separation was achieved on a 250 × 4.6 mm, 5μ, Waters symmetry column in gradient mode, with mobile phase consisting of a mixture of solution (10 mM potassium dihydrogen phosphate, pH 3.

3-D volumetric Lensfree imaging using Digital Holographic Microscopy

Despite of advancement in microscopic techniques most of the improved microscopy modalities are complex, bulky and require costly setup. Many of the modalities are not capable of live imaging.Today imaging devices are needed to be compact, cost-effective, portable, light-weight and significantly accurate and easy to handle. Digital holography has overcome few barriers but still demands costly setup. Here we demonstrate the lens free imaging which is based on “Digital In-line holography” principle and does not require bulky and costly setup. The technique uses Light emitting diode (LED) as a source of light and CCD camera to capture the hologram of the object which allow to reconstruct the image using numerical algorithm on computing machines. This technique has many applications including one in telemedicine and can address the issues related to the rural health

Review: Ion mobility spectroscopy a new method of analysis, its application and reproducibility problems

Ion Mobility Spectrometry (IMS) is a mass-selective technique in which the sample is vaporised, ionised, and with given initial velocity moved along a drift region towards a collector electrode. The drift times (milliseconds, ms) needed by the ions to reach the collector are proportional to their masses: the higher the mass, the longer the drift times. During our evaluation of ion mobility spectrometry in the screening it was found that reproducibility problem may occurred which is solve easily by using some methods, described in this review. Ion mobility spectroscopy is widely use for the drug analysis, hair analysis and other applications

STABILITY-INDICATING RP-HPLC METHOD FOR ANALYSIS OF TELMISARTAN IN THE DOSAGE FORM

A simple, rapid, precise, rapid, sensitive and reproducible reverse phase high performance liquid chromatographic (RP-HPLC) method has been developed for quantitative analysis of Telmisartan (TELM) in pharmaceutical dosage forms. Chromatographic separation of TELM and its degradation products was achieved on a C18, 250 × 4.6 mm, 5μ, Waters symmetry column

Application of Validated HPLC Method for Degradation Study of Sitagliptin and Metformin HCl

A novel and simple reverse phase liquid chromatographic method has been established for the determination of Sitagliptin and Metformin HCl and studies its degradation pattern in pharmaceutical dosage forms. Sitagliptin and Metformin HCl is used to control Type 2 Diabetes. The proposed work was performed on Younglin( S.K) isocratic System UV DetectorC18 column (150 mm 4.6 mm). A mixture of Potassium Phosphate buffer pH-3.2 with orthophosphoric acid and acetonitrile was used as mobile phase in this method with flow rate 0.7 ml/min (UV detection at 203 nm) and the method was validated as per ICH guidelines. Forced degradation studies were performed by exposing the drug Sitagliptin and Metformin HCl to acidic, alkaline, oxidation and thermal stress degradations. The proposed RP-HPLC method was found to be robust and specific and this method is suitable for the assay of pharmaceutical dosage forms as well as kinetic studies

FORMULATION AND EVALUATION OF IMMEDIATE RELEASE TABLETS OF METFORMIN HYDROCHLORIDE ON LABORATORY SCALE

The purpose of this research is to prepare metformin hydrochloride immediate release tablets by wet granulation technique. In order to obtain the best, optimized product ten different formulations were developed. Different binder, disintegrants and lubricants taken as variables. Weight variation, thickness, hardness, friability, disintegration time, in-vitro release and pharmaceutical assay were studied as response variables. Capping was observed in formulation containing PVP K-30. However, in the remaining formulation containing PVP K-90, no capping was observed. The formulation A7 was selected as optimized formulation. The different physical properties and in-vitro release profile showed best comparable with the reference product. Optimization has proven an effective tool in product development

Review: Development of forced degradation studies of drugs

Forced degradation studies show the chemical behavior of the molecule which in turn helps in the development of formulation and package. A forced degradation study is an essential step in the design of a regulatory compliant stability program for both drug substances and products, and formalized as a regulatory requirement in ICH Guideline Q1A in 1993. Forced degradation is a degradation of new drug substance and drug product at conditions more severe than accelerated conditions. It is required to demonstrate specificity of stability indicating methods and also provides an insight into degradation pathways and degradation products of the drug substance and helps in elucidation of the structure of the degradation products. Thus, this review discusses the current trends in performance of forced degradation studies by providing a strategy for conducting studies on degradation mechanisms