2 research outputs found
A comparison between characterization and biological properties of brazilian fresh and aged propolis
As propolis is a highly valued bee product, we aimed to verify the quality of aged propolis, investigating their phenolic and flavonoid composition, levels of toxic metals, radical scavenging and antimicrobial activities. Samples of fresh and aged propolis of six different beekeepers, from the same geographical location, were investigated in terms of their phenolic and flavonoid composition and levels of Pb, Cd, and Cr, as well as radical scavenging and antimicrobial activities. The two groups of propolis had similar qualitative composition by HPLC-PDA and ESI(-)-MS. Fresh propolis and aged propolis show no differences when average values of extraction yield, flavonoids, EC50, or MIC were compared and both types of propolis showed good antimicrobial activity at low concentrations. Only levels of phenolic compounds were higher in fresh propolis. The propolis samples considered in this study, aged or fresh, had similar qualitative composition, although they were collected in different periods. Samples only differed in their levels of total phenolic content. Moreover, aged propolis conserves significant radical scavenging and antimicrobial properties. We suggest that aged propolis should not be discarded but explored for alternative applications.As propolis is a highly valued bee product, we aimed to verify the quality of aged propolis, investigating their phenolic and flavonoid composition, levels of toxic metals, radical scavenging and antimicrobial activities. Material and Methods. Samples of2014FINEP - FINANCIADORA DE ESTUDOS E PROJETOSCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOFA - FUNDAÇÃO ARAUCÁRIA DE APOIO AO DESENVOLVIMENTO CIENTÍFICO E TECNOLÓsem informaçãosem informaçãosem informação10908/PPP/2006sem informaçãoThe study had financial support by Financiadora de Estudos e projetos (FINEP), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), Fundação Araucária (10908/PPP/2006), and P
Evaluation of antimicrobial activity of propolis extracts in different maturation
The discovery and synthesis of antimicrobial comprise an element of paramount
importance to health, however some of these substances have become obsolete due
to the emergence of resistant microorganisms conventional therapy. Within the forms
of treatment, natural products are an inexhaustible source of substances, including
propolis, which is known worldwide due to its antimicrobial activity. The objective of
this study was the microbiological analysis of six propolis samples collected from
regions Prudentópolis - PR , at different times of deposit hive, with up to 40 days of
deposit (New Propolis) and another with over 180 days (Propolis old). The
antimicrobial activity was observed against Pseudomonas aeruginosa, Escherichia
coli, Staphylococcus aureus, Micrococcus luteus and Enterococcus faecalis through
testing microdilution plate with colorimetric assay using resazurin. At the end of the
tests gave values ranging from 0.38 to 0.68 mg/ml to MIC new propolis and 0.34 to
1.3 mg/ml for the old to the microorganisms S. aureus and M. luteus having values
close to both of these as well as the CBM between 0.38 to 1.62 and from 0.67 to 2.6
mg/ml, respectively. Similarly, for E. faecalis MIC values between 0.76 and 2.73
mg/ml for the new propolis and 2.72 and 1.34 mg/ml of the propolis old were made,
and MBC values of 1.50 to 3.07 and 2.68 to 3.11 mg/ml, respectively, for samples
1V, 2N and 5N 5V CBM not observed for the concentrations studied. Gram- negative
microorganisms were not sensitive to propolis. We conclude that the new propolis
showed better antimicrobial activity, especially against S. aureus and M. luteus.
However, the data also show that the values of MIC and MBC were very close
between the different propolis, which was not evident reasons why the old propolis is
discarded.A descoberta e síntese de antimicrobianos compõem um elemento de suma importância para a saúde, entretanto algumas dessas substâncias tornaram-se obsoletas devido ao surgimento de micro-organismos resistentes à terapêutica convencional. Dentro das formas de tratamento, os produtos naturais são uma fonte
inesgotável de substâncias, entre elas a própolis, a qual é conhecida mundialmente
devido a sua atividade antimicrobiana. O objetivo deste trabalho foi a análise
microbiológica de seis amostras de própolis, coletadas de regiões de Prudentópolis-
PR, em tempos distintos de depósito em colmeia, uma com até 40 dias de depósito
(Própolis Nova) e outra com mais de 180 dias (Própolis Velha). A atividade
antimicrobiana foi verificada frente a Pseudomonas aeruginosa, Escherichia coli,
Staphylococcus aureus, Micrococcus luteus e Enterococcus faecalis, através do
ensaio de microdiluição em placa com teste colorimétrico usando resazurina. Ao fim
dos testes obteve-se os valores variando entre 0,38 a 0,68 mg/ml para a CIM da
própolis nova e 0,34 a 1,3 mg/ml para a velha, para os micro-organismos S. aureus
e M. luteus tendo valores próximos para ambos assim como a CBM destes, entre
0,38 - 1,62 e 0,67 – 2,6 mg/ml, respectivamente. Da mesma forma, para o E. faecalis
foram alcançados os valores de CIM entre 0,76 e 2,73 mg/ml para a própolis nova e
1,34 e 2,72 mg/ml para a própolis velha, bem como valores de CBM de 1,5 a 3,07 e
2,68 a 3,11 mg/ml, respectivamente, nas amostras 1V, 2N, 5V e 5N não se verificou
CBM para as concentrações estudadas. Os micro-organismos gram-negativos não
foram sensíveis ao própolis. Conclui-se que a própolis nova apresentou melhor ação
antimicrobiana, principalmente contra S. aureus e M. luteus. No entanto, os dados
também mostram que os valores de CIM e CBM foram bem próximos entre as
diferentes própolis, fato que não evidencia razões para que a própolis velha seja
descartada