Development of Technology for Large Scale Production of Titanium Sponge

Intensive investigations on the development of titanium metal production technology had been carried out during 1965-1975 at the Bhabha Atomic Research Centre, Bombay and at the Nuclear Fuel Complex, Hyderabad. The Defence Metallurgical Research Laboratory, Hyderabad has set up a ‘Titanium Sponge Experimental Facility’ with a capacity to produce 100 tonnes of sponge per annum in 2000 kg batches by the Kroll’s process with a view to optimising technology for large scale production.The paper presents an outline of the experimental facility and discusses the various considerations that have gone into the design of equipment, materials of construction, process selection, etc. The facility has gone into operation in March,1985. The results obtained so far are encouraging

A Hybrid Microgrid Operated by PV Wind and Diesel Generator with Advanced Control Strategy

All for a local area that gets its power from a solitary diesel generator (DG), this examination presents an efficient power energy choice for a microgrid. A twin feed enlistment generator draws power from a sun oriented photovoltaic (PV) cluster and the breeze to run this microgrid's electrical gear (DFIG). Two voltage source converters (VSCs) are sequentially coupled on the rotor side of the DFIG and share a DC transport that at last prompts the photovoltaic modules. Likewise associated with a similar DC transport as the DFIG stator is a bidirectional buck/help DC converter and a battery energy capacity (BES) to retain any overflow power. Most extreme energy collecting from the breeze and sun is accomplished by regulation of the bidirectional buck/help DC converter and the rotor side VSC. A changed form of the irritate and notice (P&O) technique is introduced for of expanding the energy result of a PV framework. Endeavors are being made to change VSC on the heap side to further develop DG's eco-friendliness. The ideal fuel-use reference DG power result may now be resolved utilizing a new, more broad methodology. Using the Sim Power Systems toolbox in MATLAB, we model and simulate many scenarios, including fluctuating wind speeds, fluctuating insolation, the impact of fluctuating load conditions on a bidirectional converter, and an unbalanced nonlinear load linked at the point of common coupling (PCC). Finding sinusoidal and balanced DG and DFIG stator currents

Synthesis, spectral characterization and anti-diabetic activity evaluation of phosphoramidate derivatives

60-68In the present work, a series of new phosphoramidate derivatives have been conveniently synthesized by using 4- chlorobenzene-sulfonyl chloride as preliminary material via substituted sulfonamides as intermediates with high yields in a short period by conventional and microwave irradiation techniques. All the synthesized compound have been characterized by spectral and elemental analyses. An in silico molecular docking study has been performed to find potent anti-diabetic drugs. In vitro anti-diabetic activity of the title compounds have also been screened by standard α-amylase inhibition assay. Some of the tested compounds have been proven to possess promising activity when compared with reference drug

Chitin Binding Proteins Act Synergistically with Chitinases in Serratia proteamaculans 568

Genome sequence of Serratia proteamaculans 568 revealed the presence of three family 33 chitin binding proteins (CBPs). The three Sp CBPs (Sp CBP21, Sp CBP28 and Sp CBP50) were heterologously expressed and purified. Sp CBP21 and Sp CBP50 showed binding preference to β-chitin, while Sp CBP28 did not bind to chitin and cellulose substrates. Both Sp CBP21 and Sp CBP50 were synergistic with four chitinases from S. proteamaculans 568 (Sp ChiA, Sp ChiB, Sp ChiC and Sp ChiD) in degradation of α- and β-chitin, especially in the presence of external electron donor (reduced glutathione). Sp ChiD benefited most from Sp CBP21 or Sp CBP50 on α-chitin, while Sp ChiB and Sp ChiD had major advantage with these Sp CBPs on β-chitin. Dose responsive studies indicated that both the Sp CBPs exhibit synergism ≥0.2 µM. The addition of both Sp CBP21 and Sp CBP50 in different ratios to a synergistic mixture did not significantly increase the activity. Highly conserved polar residues, important in binding and activity of CBP21 from S. marcescens (Sm CBP21), were present in Sp CBP21 and Sp CBP50, while Sp CBP28 had only one such polar residue. The inability of Sp CBP28 to bind to the test substrates could be attributed to the absence of important polar residues

Electrochemical determination of hydroquinone using hydrophobic ionic liquid-type carbon paste electrodes

Three types of carbon paste electrodes (CPEs) with different liquid binders were fabricated, and their electrochemical behavior was characterized via a potassium hexacyanoferrate(II) probe. 1-Octyl-3-methylimidazolium hexafluorophosphate ionic liquid (IL) as a hydrophobic conductive pasting binder showed better electrochemical performance compared with the commonly employed binder. The IL-contained CPEs demonstrated excellent electroactivity for oxidation of hydroquinone. A diffusion control mechanism was confirmed and the diffusion coefficient (D) of 5.05 × 10-4 cm2 s-1 was obtained. The hydrophobic IL-CPE is promising for the determination of hydroquinone in terms of high sensitivity, easy operation, and good durability

The RNA Binding Protein SAM68 Transiently Localizes in the Chromatoid Body of Male Germ Cells and Influences Expression of Select MicroRNAs

The chromatoid body (CB) is a unique structure of male germ cells composed of thin filaments that condense into a perinuclear organelle after meiosis. Due to the presence of proteins involved in different steps of RNA metabolism and of different classes of RNAs, including microRNAs (miRNAs), the CB has been recently suggested to function as an RNA processing centre. Herein, we show that the RNA binding protein SAM68 transiently localizes in the CB, in concomitance with the meiotic divisions of mouse spermatocytes. Precise staging of the seminiferous tubules and co-localization studies with MVH and MILI, two well recognized CB markers, documented that SAM68 transiently associates with the CB in secondary spermatocytes and early round spermatids. Furthermore, although SAM68 co-immunoprecipitated with MVH in secondary spermatocytes, its ablation did not affect the proper localization of MVH in the CB. On the other hand, ablation of the CB constitutive component MIWI did not impair association of SAM68 with the CB. Isolation of CBs from Sam68 wild type and knockout mouse testes and comparison of their protein content by mass spectrometry indicated that Sam68 ablation did not cause overall alterations in the CB proteome. Lastly, we found that SAM68 interacts with DROSHA and DICER in secondary spermatocytes and early round spermatids and that a subset of miRNAs were altered in Sam68−/−germ cells. These results suggest a novel role for SAM68 in the miRNA pathway during spermatogenesis

Ethnobotany genomics - discovery and innovation in a new era of exploratory research

We present here the first use of DNA barcoding in a new approach to ethnobotany we coined "ethnobotany genomics". This new approach is founded on the concept of 'assemblage' of biodiversity knowledge, which includes a coming together of different ways of knowing and valorizing species variation in a novel approach seeking to add value to both traditional knowledge (TK) and scientific knowledge (SK). We employed contemporary genomic technology, DNA barcoding, as an important tool for identifying cryptic species, which were already recognized ethnotaxa using the TK classification systems of local cultures in the Velliangiri Hills of India. This research is based on several case studies in our lab, which define an approach to that is poised to evolve quickly with the advent of new ideas and technology. Our results show that DNA barcoding validated several new cryptic plant species to science that were previously recognized by TK classifications of the Irulas and Malasars, and were lumped using SK classification. The contribution of the local aboriginal knowledge concerning plant diversity and utility in India is considerable; our study presents new ethnomedicine to science. Ethnobotany genomics can also be used to determine the distribution of rare species and their ecological requirements, including traditional ecological knowledge so that conservation strategies can be implemented. This is aligned with the Convention on Biological Diversity that was signed by over 150 nations, and thus the world's complex array of human-natural-technological relationships has effectively been re-organized

Target selection and annotation for the structural genomics of the amidohydrolase and enolase superfamilies

To study the substrate specificity of enzymes, we use the amidohydrolase and enolase superfamilies as model systems; members of these superfamilies share a common TIM barrel fold and catalyze a wide range of chemical reactions. Here, we describe a collaboration between the Enzyme Specificity Consortium (ENSPEC) and the New York SGX Research Center for Structural Genomics (NYSGXRC) that aims to maximize the structural coverage of the amidohydrolase and enolase superfamilies. Using sequence- and structure-based protein comparisons, we first selected 535 target proteins from a variety of genomes for high-throughput structure determination by X-ray crystallography; 63 of these targets were not previously annotated as superfamily members. To date, 20 unique amidohydrolase and 41 unique enolase structures have been determined, increasing the fraction of sequences in the two superfamilies that can be modeled based on at least 30% sequence identity from 45% to 73%. We present case studies of proteins related to uronate isomerase (an amidohydrolase superfamily member) and mandelate racemase (an enolase superfamily member), to illustrate how this structure-focused approach can be used to generate hypotheses about sequence–structure–function relationships