Clinical and radiological evaluation of elbow dysplasia in 10 dogs

Cetinkaya, Mehmet Alper/0000-0001-5097-6368;WOS: 000274637800005The study material was composed of 10 dogs from various breed, age and sex brought to clinic with complaint of forelimb lameness in which elbow dysplasia was found. After clinical and radiological examinations types and the degrees of the dysplasia were identified. Seven of the cases were surgically interfered where only symptomatic treatment was performed in three. After treatment process', results were excellent in 4, good in 4 and moderate in 2 cases. As a result it was concluded that; surgical intervention performed before the occurence of the chronic degenerative lesions was found to be efficient for the prognosis and supportive medical treatment has significant role in relieving of clinical symptoms

Proerythroblast Cells of Diamond-Blackfan Anemia Patients With RPS19 and CECR1 Mutations Have Similar Transcriptomic Signature

Diamond Blackfan Anemia (DBA) is an inherited bone marrow (BM) failure syndrome, characterized by a paucity of erythroid differentiation. DBA is mainly caused by the mutations in ribosomal protein genes, hence classified as ribosomopathy. However, in approximately 30% of patients, the molecular etiology cannot be discovered. RPS19 germline mutations caused 25% of the cases. On the other hand, CECR1 mutations also cause phenotypes similar to DBA but not being a ribosomopathy. Due to the blockade of erythropoiesis in the BM, we investigated the transcriptomic profile of three different cell types of BM resident cells of DBA patients and compared them with healthy donors. From BM aspirates BM mononuclear cells (MNCs) were isolated and hematopoietic stem cells (HSC) [CD71–CD34+ CD38mo/lo], megakaryocyte–erythroid progenitor cells (MEP) [CD71–CD34+ CD38hi] and Proerythroblasts [CD71+ CD117+ CD38+] were sorted and analyzed with a transcriptomic approach. Among all these cells, proerythroblasts had the most different transcriptomic profile. The genes associated with cellular stress/immune responses were increased and some of the transcription factors that play a role in erythroid differentiation had altered expression in DBA proerythroblasts. We also showed that gene expression levels of ribosomal proteins were decreased in DBA proerythroblasts. In addition to these, colony formation assay (CFU-E) provided functional evidence of the failure of erythroid differentiation in DBA patients. According to our findings that all patients resembling both RPS19 and CECR1 mutations have common transcriptomic signatures, it may be possible that inflammatory BM niche may have a role in DBA pathogenesis

Treatment Of Rostral Mandibular Body Fractures In 3 Cats

Three cats which had rostral mandibular body fracture just distal to canine teeth; formed the material of the study. Partial mandibulectomy and acrylic external fixator techniques were used in cases for treatment. The purpose of this study is to present some techniques that are useful for treatment of these fractures in cats.Wo