8 research outputs found

    Complete Genome Sequence of Houston Virus, a Newly Discovered Mosquito-Specific Virus Isolated from Culex quinquefasciatus in Mexico

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    ABSTRACT We fully sequenced the genome of Houston virus, a recently discovered mosquito-associated virus belonging to the newly established family Mesoniviridae. The isolate was recovered from Culex quinquefasciatus in southern Mexico, which shows that the geographic range of Houston virus is not restricted to the United States in North America

    Fecundity and morphological description of the eggs of Psorophora cyanescens (Coquillett, 1902) (Diptera: Culicidae) by scanning electron microscopy

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    Taxonomic studies on mosquitoes are based on the morphological description of larvae and adults. However, few studies have focused on the morphological description of eggs despite their taxonomic value. The description of mosquito eggs by scanning electron microscopy (SEM) allows more detailed descriptions of the ornamentation of the egg, which can provide diagnostic characters of the species. The objective of the work was to describe the eggs of Psorophora cyanescens by SEM and provide fecundity data for the species. In general, Ps. cyanescens eggs are elliptical and very wide. The exochorion is characterized by polygon pattern, where each polygon consists of longitudinal ridges joined by cross-ridges defining regular "hexagonal" areas. Each longitudinal ridge consisting by a small tubercle. The small tubercles are irregular, rectangular, rounded, or tubular. The ornamentation of the exochorion also has long and conical tubercles throughout the egg region. The micropylar apparatus located in the anterior region of the egg has a prominent, continuous and thickness collar. Psorophora cyanescens laid mature eggs at 2.5 days post-feeding. The mean number of eggs per female was 82.20 (± 13.31). This is the first study that describes the ultrastructure of the morphology of the eggs of Ps. cyanescens by SEM. With the contribution of the present work, there are five species of the genus Psorophora whose eggs morphology is described by SEM: Ps. albigenu, Ps. albipes, Ps. columbiae, Ps. cyanescens, and Ps. ferox.Los estudios taxonómicos sobre mosquitos se basan en la descripción morfológica de larvas y adultos. Sin embargo, son pocos los estudios enfocados en la descripción morfológica de los huevos a pesar de su valor taxonómico. La descripción de los huevos de mosquitos mediante microscopía electrónica de barrido (MEB) permite obtener imágenes más detalladas de la ornamentación del huevo, los cuales pueden proporcionar caracteres diagnósticos de la especie. El objetivo del trabajo fue describir los huevos de Psorophora cyanescens por MEB y proporcionar datos de fecundidad de la especie. En general, los huevos de Ps. cyanescens son de forma elíptica y muy anchos. El exocorión se caracteriza por un patrón de polígono, donde cada polígono consta de crestas longitudinales unidas por crestas cruzadas que definen áreas "hexagonales" regulares. Cada cresta longitudinal consiste en un pequeño tubérculo. Los pequeños tubérculos son irregulares, rectangulares, redondeados o tubulares. La ornamentación del exocorión también presenta largos tubérculos cónicos en toda la región del huevo. El aparato micropilar ubicado en la región anterior del huevo tiene un collar prominente, continuo y grueso. Psorophora cyanescens puso huevos maduros a los 2.5 días después de la alimentación sanguínea. El número promedio de huevos por hembra fue de 82.20 (± 13.31). Este es el primer estudio que describe la ultraestructura de la morfología de los huevos de Ps. cyanescens mediante MEB. Con el aporte del presente trabajo, son cinco las especies del género Psorophora que cuentan con la descripción de la morfología de los huevos mediante MEB: Ps. albigenu, Ps. albipes, Ps. columbiae, Ps. cyanescens, and Ps. ferox

    Survey of mosquito-borne flaviviruses in the Cuitzmala River Basin, Mexico: do they circulate in rodents and bats?

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    Abstract Background RNA viruses commonly infect bats and rodents, including mosquito-borne flaviviruses (MBFV) that affect human and animal health. Serological evidence suggests past interactions between these two mammalian orders with dengue viruses (DENV), West Nile virus (WNV), and yellow fever virus (YFV). Although in Mexico there are reports of these viruses in both host groups, we know little about their endemic cycles or persistence in time and space. Methods Rodents and bats were captured at the Cuitzmala River Basin on the Pacific coast of Jalisco state, Mexico, where MBFV, such as DENV, have been reported in both humans and bats. Samples were taken during January, June, and October 2014, at locations adjacent to the river. Tissue samples were collected from both bats and rodents and serum samples from rodents only. Highly sensitive serological and molecular assays were used to search for current and past evidence of viral circulation. Results One thousand nine hundred forty-eight individuals were captured belonging to 21 bat and 14 rodent species. Seven hundred sixty-nine liver and 764 spleen samples were analysed by means of a specific molecular protocol used to detect flaviviruses. Additionally, 708 serum samples from rodents were examined in order to demonstrate previous exposure to dengue virus serotype 2 (which circulates in the region). There were no positive results with any diagnostic test. Discussion To our knowledge, this is the first survey of rodents and only the second survey of bats from the Pacific Coast of Mexico in a search for MBFV. We obtained negative results from all samples. We validated our laboratory tests with negative and positive controls. Our findings are consistent with other empirical and experimental studies in which these mammalian hosts may not replicate mosquito-borne flaviviruses or present low prevalence. Conclusions True-negative results are essential for the construction of distribution models and are necessary to identify potential areas at risk. Negative results should not be interpreted as the local absence of MBFV in the region. On the contrary, we need to establish a long-term surveillance programme to find MBFV presence in the mosquito trophic networks, identifying the potential role of rodents and bats in viral dynamics

    Entomological and virological surveillance for dengue virus in churches in Merida, Mexico

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    ABSTRACT This study was designed to assess whether churches in endemic dengue districts in Merida, Mexico provide suitable breeding habitats for mosquitoes and are potential sites for dengue virus (DENV) transmission. Churches were inspected for immature and adult mosquitoes once every week from November 2015 to October 2016. A total of 10,997 immatures of five species were collected. The most abundant species were Aedes aegypti (6,051) and Culex quinquefasciatus (3,018). The most common source of immature Ae. aegypti were buckets followed by disposable containers. Adult collections yielded 21,226 mosquitoes of nine species. The most common species were Cx. quinquefasciatus (15,215) and Ae. aegypti (3,902). Aedes aegypti were found all year long. Female Ae. aegypti (1,380) were sorted into pools (166) and assayed for flavivirus RNA by RT-PCR and Sanger sequencing. Two pools were positive for DENV (DENV-1 and 2). In conclusion, we demonstrated that some churches in Merida are infested with mosquitoes all year long and they potentially serve as sites for DENV transmission and should therefore be considered for inclusion in mosquito and arboviruses control and surveillance efforts

    Arbovirus Surveillance near the Mexico–U.S. Border: Isolation and Sequence Analysis of Chikungunya Virus from Patients with Dengue-like Symptoms in Reynosa, Tamaulipas

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    A total of 1,090 residents of the city of Reynosa, Tamaulipas, on the Mexico–U.S. border presented at hospitals and clinics of the Secretariat of Health, Mexico, in 2015 with symptoms characteristic of dengue. Dengue virus (DENV) antigen was detected by enzyme-linked immunosorbent assay in acute sera from 134 (12.3%) patients. Sera from select patients (N = 34) were also tested for chikungunya virus (CHIKV) RNA by quantitative reverse transcription–polymerase chain reaction. Thirteen (38.2%) patients, including five DENV antigen-positive patients, were positive. Sera from three CHIKV RNA-positive patients were further assayed by virus isolation in cell culture and CHIKV was recovered on each occasion. The genome of one isolate and structural genes of the other two isolates were sequenced. In conclusion, we present evidence of CHIKV and DENV coinfections in patients who live near the Mexico–U.S. border and provide the first genome sequence of a CHIKV isolate from northern Mexico

    Surveillance for Flaviviruses Near the Mexico-U.S. Border: Co-circulation of Dengue Virus Serotypes 1, 2, and 3 and West Nile Virus in Tamaulipas, Northern Mexico, 2014-2016

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    A clinical, serological, and molecular investigation was performed to determine the presence of dengue virus (DENV) and other flaviviruses among residents of the city of Reynosa, Tamaulipas, on the Mexico–U.S. border in 2014–2016. The sample population consisted of 2,355 patients with suspected dengue, in addition to 346 asymptomatic individuals recruited during a household-based epidemiological investigation designed to identify flavivirus seroconversions. Sera were collected from patients with suspected dengue in the acute phase of illness and from asymptomatic individuals at enrollment and every 5–7 months for 19 months. Sera from suspected dengue patients were tested for DENV antigen by enzyme-linked immunosorbent assay (ELISA), and select antigen-positive sera were further tested using a serotype-specific, quantitative reverse transcription–polymerase chain reaction. Sera from the household cohort were tested for flavivirus-reactive antibodies by immunoglobulin (Ig) M and IgG ELISAs using DENV antigen. A total of 418 (17.7%) patients with suspected dengue had laboratory-confirmed DENV infections, including 82 patients who were positive for DENV RNA. The most frequently detected serotype was DENV-1 (61 patients), followed by DENV-2 (16 patients) and DENV-3 (five patients). A total of 217 (62.7%) asymptomatic individuals had flavivirus-reactive antibodies at enrollment, and nine flavivirus-naïve individuals seroconverted. Sera from a subset of dengue patients and household participants, including all those who seroconverted, were further tested by plaque reduction neutralization test, resulting in the detection of antibodies to DENV-1, DENV-2, and West Nile virus. In summary, we provide evidence for the co-circulation of multiple flaviviruses in Reynosa, Tamaulipas, on the Mexico–U.S. border

    The Use of Insecticide-Treated Curtains for Control of Aedes aegypti and Dengue Virus Transmission in “Fraccionamiento” Style Houses in México

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    Dengue, chikungunya, yellow fever, and Zika viruses transmitted by Aedes aegypti mosquitoes are major public health threats in the tropical and subtropical world. In México, construction of large tracts of “fraccionamientos” high density housing to accommodate population growth and urbanization has provided fertile ground for Ae. aegypti-transmitted viruses. We investigated the utility of pyrethroid-treated window curtains to reduce both the abundance of Ae. aegypti and to prevent dengue virus (DENV) transmission in fraccionamiento housing. Windows and doors of fraccionamiento homes in urban/suburban areas, where Ae. aegypti pyrethroid resistance associated with the Ile1016 knock down resistance (kdr) mutation in the voltage gated sodium channel gene was high, and in rural areas, where kdr resistance was low, were fitted with either insecticide-treated curtains (ITCs) or non-treated curtains (NTCs). The homes were monitored for mosquito abundance and DENV infection. ITCs reduced the indoor abundance of Ae. aegypti and the number of DENV-infected mosquitoes in homes in rural but not in urban/suburban study sites. The presence of non-treated screens also was associated with reduced numbers of mosquitoes in homes. “Super-infested” homes, yielding more than 50 mosquitoes, including DENV-infected mosquitoes, provide a significant public health risk to occupants, visitors, and people in neighboring homes
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