15 research outputs found

    Transcriptional and Morpho-Physiological Responses of Marchantia polymorpha upon Phosphate Starvation

    No full text
    Phosphate (Pi) is a pivotal nutrient that constraints plant development and productivity in natural ecosystems. Land colonization by plants, more than 470 million years ago, evolved adaptive mechanisms to conquer Pi-scarce environments. However, little is known about the molecular basis underlying such adaptations at early branches of plant phylogeny. To shed light on how early divergent plants respond to Pi limitation, we analyzed the morpho-physiological and transcriptional dynamics of Marchantia polymorpha upon Pi starvation. Our phylogenomic analysis highlights some gene networks present since the Chlorophytes and others established in the Streptophytes (e.g., PHR1–SPX1 and STOP1–ALMT1, respectively). At the morpho-physiological level, the response is characterized by the induction of phosphatase activity, media acidification, accumulation of auronidins, reduction of internal Pi concentration, and developmental modifications of rhizoids. The transcriptional response involves the induction of MpPHR1, Pi transporters, lipid turnover enzymes, and MpMYB14, which is an essential transcription factor for auronidins biosynthesis. MpSTOP2 up-regulation correlates with expression changes in genes related to organic acid biosynthesis and transport, suggesting a preference for citrate exudation. An analysis of MpPHR1 binding sequences (P1BS) shows an enrichment of this cis regulatory element in differentially expressed genes. Our study unravels the strategies, at diverse levels of organization, exerted by M. polymorpha to cope with low Pi availability

    Proyecto Curricular Comercio Internacional en Castilla y León

    No full text
    Proyecto realizado en el Centro de F.P. 'Gregorio Fernández' de Valladolid, llevado a cabo por un equipo de 2 profesores que integra el Departamento de Tecnología Administrativa y Comercio Exterior. Tienen como objetivo: Completar, concretar y adaptar el ciclo formativo de Técnico en Comercio a las necesidades de comercialización de los productos agroalimentarios en Castilla y León. Para el desarrollo del trabajo toman como base el contenido del R.D. 1655/1994 de 22 de julio, sobre el título formativo de grado medio 'Comercio', a través del siguiente proceso: -puesta en común sobre el tema con análisis y debate del contenido del R.D; -búsqueda y recopilación de material específico de C y L; -estudio exhaustivo del ciclo; -distribución del trabajo; -elaboración de materiales. Finalmente incluyen una Unidad Didáctica que, a modo de proyecto final del ciclo, trata de dar una visión de conjunto de todas las enseñanzas del ciclo. El trabajo no está publicado..Comunidad Autónoma de Castilla y León, Consejería de Educación y Cultura, Dirección General de EducaciónCastilla y LeónES

    A systematic literature review of simulation models for non-technical skill training in healthcare logistics

    No full text

    Safety of hospital discharge before return of bowel function after elective colorectal surgery

    No full text
    Background Ileus is common after colorectal surgery and is associated with an increased risk of postoperative complications. Identifying features of normal bowel recovery and the appropriateness for hospital discharge is challenging. This study explored the safety of hospital discharge before the return of bowel function. Methods A prospective, multicentre cohort study was undertaken across an international collaborative network. Adult patients undergoing elective colorectal resection between January and April 2018 were included. The main outcome of interest was readmission to hospital within 30 days of surgery. The impact of discharge timing according to the return of bowel function was explored using multivariable regression analysis. Other outcomes were postoperative complications within 30 days of surgery, measured using the Clavien-Dindo classification system. Results A total of 3288 patients were included in the analysis, of whom 301 (9 center dot 2 per cent) were discharged before the return of bowel function. The median duration of hospital stay for patients discharged before and after return of bowel function was 5 (i.q.r. 4-7) and 7 (6-8) days respectively (P < 0 center dot 001). There were no significant differences in rates of readmission between these groups (6 center dot 6 versus 8 center dot 0 per cent; P = 0 center dot 499), and this remained the case after multivariable adjustment for baseline differences (odds ratio 0 center dot 90, 95 per cent c.i. 0 center dot 55 to 1 center dot 46; P = 0 center dot 659). Rates of postoperative complications were also similar in those discharged before versus after return of bowel function (minor: 34 center dot 7 versus 39 center dot 5 per cent; major 3 center dot 3 versus 3 center dot 4 per cent; P = 0 center dot 110). Conclusion Discharge before return of bowel function after elective colorectal surgery appears to be safe in appropriately selected patients
    corecore