Effect of Turkish pollen and propolis extracts on caspase-3 activity in myeloid cancer cell lines

Purpose: To investigate the apoptosis-inducing capacity of dimethyl sulfoxide (DMSO) extracts of bee pollen and propolis in HL-60 Myeloid Cancer Cell Lines.Methods: DMSO extracts of pollen and propolis were incubated separately with HL-60 cells, and caspase-3 activity evaluated. In order to determine the cell cycle characteristics of HL-60 cells with and without extracts of pollen and propolis, the cells were analysed using flow cytometry.Results: The DMSO extract of propolis (0.5 mg/mL) increased apoptosis from undetectable levels to 60.1 %, while maintaining cell viability. The DMSO extract of pollen (2 mg/ml) increased apoptosis from undetectable levels to 52.2 % while decreasing cell viability by 62 %. Caspase-3 activity in HL-60 cells incubated with DMSO extracts of pollen and propolis were 3.6- to 12-fold higher than in controls.Conclusion: Turkish pollen and propolis individually increase apoptosis and the activity of caspase-3 in HL-60 cells. This finding indicates that bee products may have beneficial effects in the treatment of cancer.Keywords: Pollen, Propolis, Apoptosis, Caspase-3, Myeloid Cance

Prostat karseri hücre hatlarında dosetaksel ve zoledronik asit uygulamasının additif / sinerjistik antitümoral etkileri

Bu tezin, veri tabanı üzerinden yayınlanma izni bulunmamaktadır. Yayınlanma izni olmayan tezlerin basılı kopyalarına Üniversite kütüphaneniz aracılığıyla (TÜBESS üzerinden) erişebilirsiniz.[Abstarct Not Available

Scattering from chiral cylinders of arbitrary cross-sections above a ground plane

The main purpose of this work is to solve the problem of electromagnetic scattering from a chiral cylinder of arbitrary cross-section above an infinite perfect electric conducting (PEC) plane. Using image theory, this problem is reduced to two chiral cylinders in free-space. Surface equivalence principle is used to obtain three different equivalent problems for this two-cylinder problem. Then, the method of moments is used to solve these equivalent problems numerically. It is known that the image of a chiral body through a ground plane is another chiral body with the same permittivity and permeability but opposite chirality. Using this property, the two-body problem in the moment method may be reduced to a one-body problem with a complicated moment matrix. Computed numerical results include scattered fields and equivalent surface currents on the cylinder.Turkish Academy of Science

Scattering by a chiral cylinder of arbitrary cross section above a dielectric half-space

A simple numerical solution for electromagnetic scattering from a 2-D homogeneous chiral cylinder of arbitrary cross section placed above a dielectric half-space (DHS) is presented. A modified surface equivalence principle is used to obtain coupled electric field integral equations. For feasible application of method of moments, a perturbation method is used where a strip of finite width approximates the surface of the infinite half-space. The perturbed currents on the interface are expressed as a difference of the currents with the cylinder present and the exact solution (cylinder removed). The excitation vector now contains the reflected field from the DHS in addition to the incident field. Transverse magnetic (TM) and transverse electric (TE) excitations are treated. Computed results include perturbed currents on the dielectric interface and the scattered fields. Excellent agreement is observed for special cases available in the literature

Evaluation of protein tyrosine phosphatase activity in patients with acute leukemia

Protein tyrosine phosphatases regulate physiological processes including growth, differentiation, metabolism and the cell cycle. Together with tyrosine kinases, they control the phosphorylation state of tyrosine residues of signaling proteins. An increased level of protein phosphorylation results in abnormal proliferation and many cancer types show a mutation or deletion of a protein tyrosine phosphatase gene. In this study we evaluated the protein tyrosine phosphatase activity in acute leukemia patients. Tyrosine phosphatase activity in bone marrow mononuclear cells of acute leukemia patients was measured using a tyrosine phosphatase assay system kit and compared with a control group. We found that tyrosine phosphatase activity in acute leukemia patients was high compared to the controls. According to subgroups of acute leukemia, tyrosine phosphatase activity in the AML-M2 subgroup was high compared to the controls. The effect of increased level of protein tyrosine phosphatase activity on leukemogenesis needs further evaluation. Studies in a large group of patients are needed to emphasize the importance of tyrosine phosphatase activity in acute leukemia patients

Evaluation of protein tyrosine phosphatase activity in patients with acute leukemia

WOS: 000316428000016PubMed: 23788968Protein tyrosine phosphatases regulate physiological processes including growth, differentiation, metabolism and the cell cycle. Together with tyrosine kinases, they control the phosphorylation state of tyrosine residues of Signaling proteins. An increased level of protein phosphorylation results in abnormal proliferation and many cancer types show a mutation or deletion of a protein tyrosine phosphatase gene. in this study we evaluated the protein tyrosine phosphatase activity in acute leukemia patients. Tyrosine phosphatase activity in bone marrow mononuclear cells of acute leukemia patients was measured using a tyrosine phosphatase assay system kit and compared with a control group. We found that tyrosine phosphatase activity in acute leukemia patients was high compared to the controls. Wording to subgroups of acute leukemia, tyrosine phosphatase activity in the AML-M2 subgroup was high compared to the controls. the effect of increased level of protein tyrosine phosphatase activity on leukemogenesis needs further evaluation. Studies in a large group of patients are needed to emphasize the importance of tyrosine phosphatase activity in acute leukemia patients

Wernicke's encephalopathy in a patient with unresectable gastric carcinoma and literature review

Rakici, Sema Yilmaz/0000-0002-5543-9761; Yazici, Zihni Acar/0000-0003-1603-6545WOS: 000349897200187PubMed: 25785154Wernicke's encephalopathy (WE) is a disease classically associated with nutrition deficiency. It is characterized by typical symptoms like confusion, ataxia and ophthalmoparesis, and developes due to thiamine deficieny in alcoholic patients. Recently, it has been shown that WE could ocur in patients with gastric carcinoma without a history of alcohol use. in this paper, we have made some suggestions about early diagnosis, treatment and prevention of WE by discussing the development of WE in a patient with unresectable gastric carcinoma, who had been inpatient for a long time and given radiotherapy and chemotherapy