4,501 research outputs found
Fruit Flies Provide New Insights in Low-Radiation Background Biology at the INFN Underground Gran Sasso National Laboratory (LNGS)
Deep underground laboratories (DULs) were originally created to host particle, astroparticle or nuclear physics experiments requiring a low-background environment with vastly reduced levels of cosmic-ray particle interference. More recently, the range of science projects requiring an underground experiment site has greatly expanded, thus leading to the recognition of DULs as truly multidisciplinary science sites that host important studies in several fields, including geology, geophysics, climate and environmental sciences, technology/instrumentation development and biology. So far, underground biology experiments are ongoing or planned in a few of the currently operating DULs. Among these DULs is the Gran Sasso National Laboratory (LNGS), where the majority of radiobiological data have been collected. Here we provide a summary of the current scenario of DULs around the world, as well as the specific features of the LNGS and a summary of the results we obtained so far, together with other findings collected in different underground laboratories. In particular, we focus on the recent results from our studies of Drosophila melanogaster, which provide the first evidence of the influence of the radiation environment on life span, fertility and response to genotoxic stress at the organism level. Given the increasing interest in this field and the establishment of new projects, it is possible that in the near future more DULs will serve as sites of radiobiology experiments, thus providing further relevant biological information at extremely low-dose-rate radiation. Underground experiments can be nicely complemented with above-ground studies at increasing dose rate. A systematic study performed in different exposure scenarios provides a potential opportunity to address important radiation protection questions, such as the dose/dose-rate relationship for cancer and non-cancer risk, the possible existence of dose/dose-rate threshold(s) for different biological systems and/or end points and the possible role of radiation quality in triggering the biological response
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The pgip family in soybean and three other legume species: evidence for a birth-and-death model of evolution
Polygalacturonase-inhibiting proteins (PGIPs) are leucine-rich repeat (LRR) plant cell wall glycoproteins involved in plant immunity. They are typically encoded by gene families with a small number of gene copies whose evolutionary origin has been poorly investigated. Here we report the complete characterization of the full complement of the pgip family in soybean (Glycine max [L.] Merr.) and the characterization of the genomic region surrounding the pgip family in four legume species. Results: BAC clone and genome sequence analyses showed that the soybean genome contains two pgip loci. Each locus is composed of three clustered genes that are induced following infection with the fungal pathogen Sclerotinia sclerotiorum (Lib.) de Bary, and remnant sequences of pgip genes. The analyzed homeologous soybean genomic regions (about 126 Kb) that include the pgip loci are strongly conserved and this conservation extends also to the genomes of the legume species Phaseolus vulgaris L., Medicago truncatula Gaertn. and Cicer arietinum L., each containing a single pgip locus. Maximum likelihood-based gene trees suggest that the genes within the pgip clusters have independently undergone tandem duplication in each species. Conclusions: The paleopolyploid soybean genome contains two pgip loci comprised in large and highly conserved duplicated regions, which are also conserved in bean, M. truncatula and C. arietinum. The genomic features of these legume pgip families suggest that the forces driving the evolution of pgip genes follow the birth-and-death model, similar to that proposed for the evolution of resistance (R) genes of NBS-LRR-type
Antibiotic susceptibility profiles of ropy slime-producing Leuconostoc mesenteroides isolated from cooked meat products
The transfer of antibiotic resistance via the food chain is a global concern. Nevertheless, more attention is required to non-pathogenic strains, such as spoilage bacteria, which could transmit genes to pathogens. Although Lactic Acid Bacteria are microorganisms generally recognized as safe, Leuconostoc mesenteroides may reach and maintain high concentration levels on the surface of cooked products and ready-to-eat products throughout the entire shelf life. It is therefore important to consider the possibility for this species to carry antibiotic- resistance genes. The present research deals with the antibiotic susceptibility profile of strains of L. mesenteroides, isolated from vacuum packaged cooked meat products. In this study, the antimicrobial susceptibility of L.mesenteroides, previously isolated from cooked ham, was investigated through disk diffusion assay according to CLSI standards. Isolated strains from ready-to-eat food show high levels of resistance to ampicillin and methicillin and, according to a settled panel of 21 antibiotics, the antibiotic resistance was demonstrated for the 50% of the tested molecules
Geomatics for Integrated Coastal Zone Management: multitemporal shoreline analysis and future regional perspective for the Portuguese Central Region
Shoreline mapping and change detection are critical for Integrated Coastal Zone Management (ICZM) and all that it represents. This research utilized previous studies that combined both Remote Sensing and Geographical Information System (GIS) techniques to assess, map and forecast shoreline evolution from short-term perspectives. The study area is located in the central region of Portugal, between the counties of Ovar and Marinha Grande (circa 140 km) and the time period assessed was from 1984 to 2011. Historical data were used to calculate advance and retreat rates in order to support environmental scenarios for the Portuguese Central Region’s Coastal Management Plan. To ensure accuracy, a repeatable procedure was validated using Landsat TM and ETM+ satellite images, which were subsequently enhanced and elaborated by Remote Sensing analyses to detect and extract shorelines. They were subsequently integrated within an Esri ArcGIS software application (DSAS - Digital Shoreline Analysis System) to determine and predict rates of coastline change. Graphical DSAS plots identified coastline phases and shifts and were used to simulate the 2022 coastline scenario. These results will be integrated into the Coastal Zone Management Plan (Horizon – 2022). Importantly this methodological planning approach provides visual coastline change information for regional decision-makers and stakeholders
The performance of three different methods in detecting caries and predict severity around composite restorations
Understanding the Impact of Spatio-Temporal Resolution in Soil Moisture-Data Assimilation Systems for Flash Flood Early Warning
Shoreline to Height (S2H): an algorithm to monitor reservoirs\u2019 water height from satellite images. A flood risk management application
Effect of lactic acid bacteria on the quality of beef hamburger under different storage conditions
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