Validation of HPLC Method for the Determination of 5-hydroxymethylfurfural in Pestil, Köme, Jam, Marmalade and Pekmez

This study represents a high performance liquid chromatography HPLC method for the detection of 5-hydroxymethylfurfural in pestil, köme, jam and pekmez samples. The linearity, selectivity, decision limit, detection capability, detection limit, quantification limit, precision, recovery, ruggedness and measurement uncertainty of the method were determined. The developed method, simple and accurate, showed good recovery values 97-108% . The accuracy of the method expressed with the relative standard deviation was below 6%. The detection limit and quantification limit were 0.03 mg/kg and 0.10 mg/kg, respectively. HMF levels in pestil, köme, jam, marmalade and pekmez samples were determined using the validated metho

Hayvan Gübresinden Elde edilen Sıvı Solucan Gübresinin İz ve Besin Elementleri Açısından İncelenmesi

Sürdürülebilir tarımın karşı karşıya olduğu en önemli sorunlardan biri kaliteli gübre teminin ve gübrelemenin uygulamasında karşılaşılan sorundur. Son yıllarda bir yandan doğal yöntemlerle elde edilen gübrenin miktarı artırılmaya çalışılırken; diğer yandan farklı tip gübre (katı doğal, sıvı doğal, biyo-doğal vb..) çeşitlerinin elde edilmesine yönelik çalışmaların artarak devam ettiği görülmektedir. Bu çalışmanın amacı, fermente olmuş doğal hayvan gübresi ile beslenen solucanlardan (Eisenia fetida) elde edilen sıvı gübredeki (vermiwash) bazı besin elementleri ve metallerin zamana (30 gün, 31-6 gün) bağlı değişimlerinin belirlenmesidir. Bu amaçla, doğal meralarda otlayan ineklerden elde edilmiş ve en az 12 ay süreyle fermente olmuş hayvan gübresi ile Eisenia fetida solucanı beslenmiş ve besleme sırasında elde edilen sıvı solucan gübresindeki bazı makro ve mikro besin elementleri analiz edilmiştir. Sağım süresi arttıkça sıvı solucan gübresindeki N, K, Ca, Mg, Na, Fe, Ni, Cu, Zn, Hg, Sb değerleri istatistiksel olarak önemli seviyede azalırken; Co, As, Ag, Cd, Pb, Al, Se, Mo, Li ve Be değerleri istatistiksel olarak önemli seviyede artmıştır

Investigation of Vermiwash Obtained from California Red Worm Which Fed Cow Dung in terms of Trace and Nutrient Elements

Sürdürülebilir tarımın karşı karşıya olduğu en önemli sorunlardan biri kaliteli gübre teminin ve gübrelemenin uygulamasında karşılaşılan sorundur. Son yıllarda bir yandan doğal yöntemlerle elde edilen gübrenin miktarı artırılmaya çalışılırken; diğer yandan farklı tip gübre (katı doğal, sıvı doğal, biyo-doğal vb..) çeşitlerinin elde edilmesine yönelik çalışmaların artarak devam ettiği görülmektedir. Bu çalışmanın amacı, fermente olmuş doğal hayvan gübresi ile beslenen solucanlardan (Eisenia fetida) elde edilen sıvı gübredeki (vermiwash) bazı besin elementleri ve metallerin zamana (30 gün, 31-6 gün) bağlı değişimlerinin belirlenmesidir. Bu amaçla, doğal meralarda otlayan ineklerden elde edilmiş ve en az 12 ay süreyle fermente olmuş hayvan gübresi ile Eisenia fetida solucanı beslenmiş ve besleme sırasında elde edilen sıvı solucan gübresindeki bazı makro ve mikro besin elementleri analiz edilmiştir. Sağım süresi arttıkça sıvı solucan gübresindeki N, K, Ca, Mg, Na, Fe, Ni, Cu, Zn, Hg, Sb değerleri istatistiksel olarak önemli seviyede azalırken; Co, As, Ag, Cd, Pb, Al, Se, Mo, Li ve Be değerleri istatistiksel olarak önemli seviyede artmıştır.One of the most important problems facing sustainable agriculture is the supply of quality fertilizers and the problems encountered in the application of fertilizers. In the last years, while trying to increase the amount of fertilizers obtained by natural methods, On the other hand it has been observed that studies for obtaining different types (solid natural, liquid natural, bio-natural etc.) of fertilizer have continued increasing. The aim of this study is to determine time depend (30 days, 31-60 days) changes of some nutrients and metals in vermiwash obtained from Eisenia fetida fed with fermented natural cow dung. For this purpose, Eisenia fetida were fed with fermented cow dung which obtained from cows grazing in natural habitat and obtained vermiwash were analyzed to determined some macro and micronutrient elements. As a result of the study, while the values of N, K, Ca, Mg, Na, Fe, Ni, Cu, Zn, Hg, Sb in vermiwash decreased at a statistically significant level depending on the time; Co, As, Ag, Cd, Pb, Al, Se, Mo, Li and Be were increased at statistically significant levels

Single-Laboratory Validation for the Determination of Aflatoxin B-1, B-2, G(1), and G(2) in Foods Based on Immunoaffinity Column and Liquid Chromatography with Postcolumn Derivatization and Fluorescence Detection

This study presents a method validation procedure for the determination of aflatoxin B-1, B-2, G(1), and G(2) in hazelnut, hazelnut paste, walnut, peanut, pistachio, corn, and wheat. The method consisting of clean-up with immunoaffinity column, high performance liquid chromatography with postcolumn derivatization and fluorescence detection was validated in accordance with Commission Regulation 2004/882/EC. The selectivity, linearity, decision limit, detection capability, detection and quantification limits, precision, recovery, ruggedness, and measurement uncertainty of the method were determined. The limit of detection and limit of quantification values (mu g/kg) were: aflatoxin B-1, 0.02, 0.07; aflatoxin B-2, 0.01, 0.02; aflatoxin G(1), 0.02, 0.07; and aflatoxin G(2), 0.01, 0.03. The relative standard deviation values for the repeatability and within-laboratory reproducibility were below 4 and 5 %, respectively. The recovery values of the spiked samples ranged from 80 to 105 %. These results complied with minimum performance criteria established by regulation 2006/401/EC. Therefore, the procedure can be implemented for the routine analysis of aflatoxins in the studied matrices

Chemical Composition, Antimicrobial and Antioxidant Activities of Essential Oil from Pedicularis condensata BIEB

The chemical composition of the essential oil obtained from the dried aerial parts of Pedicularis condensata was analyzed by GC-FID and GC-MS. Thirty-eight components have been identified in the essential oil of P. condensata. The major compounds of the essential oil were pentacosane 21.28% , hexadecanoic acid 18.48% and tricosane 13.70% . The antimicrobial activity of the essential oil was also investigated and it showed moderate antimicrobial and antifungal activites aganist twelve gram negative bacteria and five fungi. The amount of total phenolic and DPPH reducing activity quantified essential oil in P. condensata were found as 198.28 GGA/L and 10.90 % respectivel

Chemical Composition of Pistacia terebinthus L. and its Phytochemical and Biological Properties

Vitamin C, enzyme activities, phenolic compounds, antioxidant capacity, antimicrobial activity, and essential oil analyses of ripe and unripe fruits of P. terebinthus were investigated. Vitamin C amounts of ripe and unripe fruits were 63.2 and 15.4 mg/100g, respectively. The main phenolic compounds of unripe and ripe fruits are rutin, syringic acid, and gallic acid. It was determined that the enzyme inhibitor activities in the ripe and unripe fruits were 0.136 mg/mL and 2.14 mg/mL. In all of the free radical scavenging (DPPH and ABTS) activity, ferric (III) ion reducing antioxidant power (FRAP) capacity, total phenolic substance amounts (TPC), total flavonoid substance amounts (TFC), and total antioxidant activity (TAC) antioxidant methods analyzed with plant parts, the methanol extracts obtained from the ripe fruits of the P. terebinthus showed higher antioxidant properties than the methanol extracts obtained from the unripe fruits. Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922 showed antimicrobial activity against microorganisms, while methanol extracts obtained from unripe fruit samples did not show antimicrobial activity against the microorganisms used. The chemical grade with the most compounds in the essential oils of P. terebinthus were monoterpenoids, sesquiterpenes, and monoterpenes in unripe and ripe fruits, respectively. The main components were α-pinene with 22.8% and 27.3% ratios in unripe and ripe fruits, respectively

Antimicrobial, Antioxidant, and Phytochemical Activities of Rhus coriaria L. and its Phenolic Compounds and Volatile Component Analyses

Volatile oil analysis, phenolic constituents, antioxidant capacity, antimicrobial activity, vitamin C, and enzyme activities of the fruits of Rhus coriaria L. were studied. The chemical with the highest percentage was sesquiterpene hydrocarbons with 40.4%. The major compound was detected as caryophyllene (36.9%). The main phenolic constituents of fruit samples were gallic acid, syringic acid, protocatechuic acid, and 4-hydroxybenzoic acid. The highest phenolic constituents of fruits were gallic acid. Ferric (III) ion reducing antioxidant power (FRAP) capacity (14.9 mg FeSO4 eq./g), free radical scavenging (ABTS) capacity (68.8 mg AA eq./g), ABTS % inhibition rate (98.0%), free radical scavenging (DPPH) (53.1 mg AA eq./g), and DPPH % inhibition (79.6%) amounts were determined in antioxidant capacities of the samples. The bioactive component contents of the samples were total antioxidant amounts (TAC) (32.8 mg GA/g), total flavonoid substance amounts (TFC) (73.8 mg QE eq./g), and total phenolic substance amounts (TPC) (41.4 mg GA eq./g). The results of the antimicrobial activity analysis of R. coriaria fruit samples showed antimicrobial activity against Staphylococcus aureus and Listeria monocytogenes microorganisms. The amount of vitamin C and enzyme inhibitor activity in the fruits of R. coriaria were determined as 35.5 mg/100 g and 0.07 mg/mL, respectively

Volatile Constituents and Biological Properties of Essential Oils from Aerial Parts of Gentiana gelida BIEB

WOS: 000488602900009PubMed: 31178458This work reports the chemical composition, antimicrobial and antioxidant activities of essential oils from the aerial parts of Gentiana gelida BIEB. for the first time in literature. The oils from the aerial parts (flower, leaf and stem) were obtained by Clevenger-type apparatus and characterized by GC-FID and GC-MS. While tricosane (21.67%) was the main component of flower oil, hexadecanoic acid was the most abundant component of leaf and stem oils in ratios 26.46% and 31.89%, respectively. Additionally, all essential oils of G. gelida were investigated for their antimicrobial activity against eight Gram negative bacteria, four Gram positive bacteria and five fungi, using agar dilution method and antioxidant activities by using 2,2-diphenyl-1-picrylhydrazyl radical scavenging and Folin-Ciocalteu assays. The flower oil of G. gelida showed stronger antimicrobial and antioxidant activities than those of stem and leaf. The amount of total phenolic content and scavenging activity of the flower oil were found 525.35 +/- 8.24 mg GAE/L and 49.30 +/- 1.25%, respectively