Selection of recombinant antibodies against Lawsonia intracellularis

Proliferative enteropathy, caused by the obligate intracellular bacterium Lawsonia intracellularis (McOrist et al., 1995), is a widely distributed disease throughout the world causing substantial economic loss. The diagnostics of the disease is complicated by limited value of serological surveys as well as the fact that L. intracellularis can only be cultured in cell cultures

Priprava rekombinantniho proteinu p25 viru Maedi-Visna a jeho vyuziti pri priprave scFv protilatek.

Available from STL, Prague, CZ / NTK - National Technical LibrarySIGLECZCzech Republi

Selection of recombinant antibodies against Lawsonia intracellularis

Proliferative enteropathy, caused by the obligate intracellular bacterium Lawsonia intracellularis (McOrist et al., 1995), is a widely distributed disease throughout the world causing substantial economic loss. The diagnostics of the disease is complicated by limited value of serological surveys as well as the fact that L. intracellularis can only be cultured in cell cultures.</p

Taxonomic update for mammalian anelloviruses (family Anelloviridae)

International audienceAnelloviruses are small negative-sense single-stranded DNA viruses with genomes ranging in size from 1.6 to 3.9 kb. The family Anelloviridae comprised 14 genera before the present changes. However, in the last five years, a large number of diverse anelloviruses have been identified in various organisms. Here, we undertake a global analysis of mammalian anelloviruses whose full genome sequences have been determined and have an intact open reading frame 1 (ORF1). We established new criteria for the classification of anelloviruses, and, based on our analyses, we establish new genera and species to accommodate the unclassified anelloviruses. We also note that based on the updated species demarcation criteria, some previously assigned species (n = 10) merge with other species. Given the rate at which virus sequence data are accumulating, and with the identification of diverse anelloviruses, we acknowledge that the taxonomy will have to be dynamic and continuously evolve to accommodate new members

Taxonomic updates for the genus Gyrovirus (family Anelloviridae):recognition of several new members and establishment of species demarcation criteria

International audienceThe genus Gyrovirus was assigned to the family Anelloviridae in 2017 with only one recognized species, Chicken anemia virus. Over the last decade, many diverse viruses related to chicken anemia virus have been identified but not classified. Here, we provide a framework for the classification of new species in the genus Gyrovirus and communicate the establishment of nine new species. We adopted the 'Genus + freeform epithet' binomial system for the naming of these species

In-Depth Characterization of Live Vaccines Used in Europe for Oral Rabies Vaccination of Wildlife

<div><p>Although rabies incidence has fallen sharply over the past decades in Europe, the disease is still present in Eastern Europe. Oral rabies immunization of wild animal rabies has been shown to be the most effective method for the control and elimination of rabies. All rabies vaccines used in Europe are modified live virus vaccines based on the Street Alabama Dufferin (SAD) strain isolated from a naturally-infected dog in 1935. Because of the potential safety risk of a live virus which could revert to virulence, the genetic composition of three commercial attenuated live rabies vaccines was investigated in two independent laboratories using next genome sequencing. This study is the first one reporting on the diversity of variants in oral rabies vaccines as well as the presence of a mix of at least two different variants in all tested batches. The results demonstrate the need for vaccine producers to use new robust methodologies in the context of their routine vaccine quality controls prior to market release.</p></div

Analysis of the eight vaccine batches using whole genome sequencing with the Illumina MiSeq 2000 platform.

<p>Abbreviations: N, nucleoprotein; P, phosphoprotein; M, matrix protein.</p><p>a) Data show the mean percentage of SADB19/SAG2 mutations for the first half of rabies genome by comparison with the SAD Bern genomic sequence (EF206708) which was used as a reference for all analysis.</p><p>Analysis of the eight vaccine batches using whole genome sequencing with the Illumina MiSeq 2000 platform.</p

Analysis of the nine vaccine sequences using 454 pyrosequencing.

<p>Abbreviations: N, nucleoprotein; P, phosphoprotein; M, matrix protein.</p><p>a) Data show the mean percentage of SADB19/SAG2 mutations for the first half of rabies genome by comparison with the SAD Bern genomic sequence (EF206708) which was used as a reference for all analysis.</p><p>Analysis of the nine vaccine sequences using 454 pyrosequencing.</p

Characteristics of primers used for the reverse transcription and PCR amplification of viral RNA prior to 454 pyrosequencing.

<p>Abbreviations: For, forward; Rev, reverse.</p><p>Characteristics of primers used for the reverse transcription and PCR amplification of viral RNA prior to 454 pyrosequencing.</p