Targeting the scaffolding role of LSD1 (KDM1A) poises acute myeloid leukemia cells for retinoic acid–induced differentiation

The histone demethylase LSD1 is deregulated in several tumors, including leukemias, providing the rationale for the clinical use of LSD1 inhibitors. In acute promyelocytic leukemia (APL), pharmacological doses of retinoic acid (RA) induce differentiation of APL cells, triggering degradation of the PML-RAR oncogene. APL cells are resistant to LSD1 inhibition or knockout, but targeting LSD1 sensitizes them to physiological doses of RA without altering of PML-RAR levels, and extends survival of leukemic mice upon RA treatment. The combination of RA with LSD1 inhibition (or knockout) is also effective in other non-APL, acute myeloid leukemia (AML) cells. Nonenzymatic activities of LSD1 are essential to block differentiation, while RA with targeting of LSD1 releases a differentiation gene expression program, not strictly dependent on changes in histone H3K4 methylation. Integration of proteomic/epigenomic/mutational studies showed that LSD1 inhibitors alter the recruitment of LSD1-containing complexes to chromatin, inhibiting the interaction between LSD1 and the transcription factor GFI1

Pilot study of the multicentre DISCHARGE trial: image quality and protocol adherence results of computed tomography and invasive coronary angiography (vol 30, pg 1997, 2020)

The original version of this article, published on 16 December 2019, unfortunately contained two mistakes.Cardiovascular Aspects of Radiolog

Exploratory study on the use of haptic feedback for graphomotricity enhancement

Handwriting ability is crucial for children's overall development, as it helps improve their sensory, motor, and cognitive skills. The link between the writing task and theories of Embedding seems to be evident: writing can be considered as an action experience, a representational form of movement that involves physical qualities and influences cognitive processes; as an action experience it shapes the perception of context throughout life. However, writing involves a range of complex skills, such as memory, language, and hand-eye coordination, making it a challenging task to learn, particularly for children who struggle with learning disabilities like intellectual disabilities, motor disabilities or Specific Learning Disorders. In recent years, newly developed haptic guidance systems show potential in aiding both adults and children in acquiring the skill of handwritin

Minilaparotomic myomectomy for large symptomatic uterine myomas: A prospective study

Aim. The aim of this paper was to evaluate the feasibility, morbidity, and reproductive performance of fertile women undergoing minilaparotomic myomectomy for large uterine myomas. Methods. Ninety-nine consecutive women with symptomatic myomas underwent myomectomy through a skin incision ≤8 cm. Operative, postoperative and reproductive data were prospectively collected. Results. Median (range) age and Body Mass Index (BMI) were 37 years (23-44) and 23 (18-43), respectively. Median (range) myoma diameter was 7 cm (4-20), and the median number of myomas removed was 1 (range 1-31). Myomas were intramural in 76 (76%) cases. Median incision length was 7 cm (range 4-13) and median duration of surgery was 70 min (range 40-180). Operative time and length of skin incision were not correlated with the progressive number of interventions. An incision larger than 8 cm was necessary in 7 (7%) patients and the length of incision was significantly correlated with the diameter of the largest myoma (P12 cm (P1 myoma (P12 cm. This technique is a mini-invasive option to treat patients with large and multiple myomas

Haptic Feedback to Support the Conceptualization of the Shape of Virtual Objects: An Exploratory Study

Virtual museum systems have been shown to play a key role in enhancing visitor’s experience and increasing the accessibility of cultural artifacts. In this context, the use of haptic interfaces based on force feedback could increase the level of immersivity of these systems and the quality of the interaction between visitors and cultural artifacts, introducing tactile information that could enrich the experience of all people, also in the case of visitors with disability. However, HD present limits concerning the ease of use of the device. This paper provides the results of an exploratory research carried out within the Research center of Teaching and learning, Disability and Educational Technology of the University of Macerata (TIncTec), consisting in two studies. Study 1 aims to analyze the learning performance of people with and without disabilities concerning this device. Study 2 aims to assess whether HD can provide useful support for conceptualizing the shape of virtual objects. To this end, we considered the 6 DoF high fidelity force feedback Geomagic Touch X by 3D system. A total of 30 people has been involved, including both children and adults with and without disabilities. The considered VR applications were developed in Unity 3D, using the 3D Systems Openhaptics Unity Plugin. The results provide useful insights for the design of future HD-based applications, showing how haptic technology can properly support visitors with disability in virtual museum environments in an inclusive perspective

Discrimination of the enantiomers of biphenylic derivatives in micellar aggregates formed by chiral amidic surfactants

The chirality of micellar aggregates formed by surfactants derived from L-proline was investigated by using two chiral biphenylic derivatives as probes of chirality. The investigation carried out by H-1 NMR, circular dichroism, and HPLC on chiral phase demonstrates that chiral recognition may occur in sites of the aggregates far from the head-group stereogenic centers and it is due to interaction with a whole region of the aggregate rather than with a single monomer in the aggregate. Subtle changes of the structure of the monomer influence the aggregation properties of the surfactants and its expression of chirality. (C) 2007 Elsevier Ltd. All rights reserved

An oenothera biennis cell cultures extract endowed with skin anti-ageing activity improves cell mechanical properties

Skin aging is a very well-known process setting a gradual worsening of skin mechanical features due to a decline in the production of the extra-cellular matrix machinery and to a concurrent change in the contraction process. To slow this progression, it is crucial to induce the expression of several proteins able to promote elastic fibers formation and tissue repair. Here, the Oenothera bi-ennis cell culture aqueous extract has been investigated from a chemical point of view and then it was tested in vitro, in cell, and in ex vivo experiments as adjuvant in counteracting skin aging. Accordingly, it has been shown that the Oenothera biennis extract was able, by increasing MYLK gene expression, to promote matrix collagen contraction, actin polymerization, and the production of essential ECM proteins

Santa Maria in Portuno nella valle del Cesano

il volume rende conto di 5 anni di scavi e ricerche condotti dal Dipartimento di Archeologia di Bologna nel sito romano e poi medievale occupato dalla chiesa di S. Maria in Portuno, presso Corinaldo (An)