742 research outputs found
Snapshot of a Bacterial Microbiome Shift during the Early Symptoms of a Massive Sponge Die-Off in the Western Mediterranean
30 páginas, 7 figuras, 1 tabla.Ocean warming is affecting marine benthic ecosystems through mass mortality events
that involve marine invertebrates, in particular bivalves, corals, and sponges. Among
these events, extensive die-offs of Ircinia fasciculata sponges have been recurrently
reported in western Mediterranean. The goal of our study was to test whether the
temperature-related mass sponge die-offs were associated with or preceded by an
early unbalanced bacterial microbiome in the sponge tissues. We took advantage of
the early detection of disease and compared the microbiomes of healthy vs. early
diseased I. fasciculata tissues. Our results showed a microbiome shift in early diseased
tissues. The abundance of Gammaproteobacteria and Acidobacteria increased and
that of Deltaproteobacteria decreased in diseased vs. healthy tissues. The change in
community composition was also noticeable at the operational taxonomic unit (OTU)
level. Diseased tissues contained more bacterial sequences previously identified in
injured or stressed sponges and corals than healthy tissues. Bacterial diversity increased
significantly in diseased tissues, which contained a higher number of low abundance
OTUs. Our results do not support the hypothesis of one particular pathogen, whether a
Vibrio or any other bacteria, triggering the Northwestern Mediterranean mass mortalities
of I. fasciculata. Our data rather suggest an early disruption of the bacterial microbiome
balance in healthy sponges through a shift in OTU abundances, and the purported
consequent decline of the sponge fitness and resistance to infections. Opportunistic
bacteria could colonize the sponge tissues, taking benefit of the sponge weakness,
before one or more virulent pathogens might proliferate ending in the mass sponge
die-off.AcknowledgmentsThis study has partially been funded by projects MarSymbiOmics (MINECO, I+D+I ofExcellence, CTM2013-43287-P), the Benthic Ecology Consolidate Team 2014-SGR-120(Generalitat de Catalunya), and BluePharm Train FP7 People-INT, to MU The work of PGwas supported by the Agence Nationale de la Recherche (ANR) through the projectsEUREKA (ANR-14-CE02-0004-01).Peer reviewe
Using Wavelets to reject background in Dark Matter experiments
A method based on wavelet techniques has been developed and applied to
background rejection in the data of the IGEX dark matter experiment. The method
is presented and described in some detail to show how it efficiently rejects
events coming from noise and microphonism through a mathematical inspection of
their recorded pulse shape. The result of the application of the method to the
last data of IGEX is presented.Comment: 14 pages, 8 figures. Submitted to Astrop. Phy
Neutron background at the Canfranc Underground Laboratory and its contribution to the IGEX-DM dark matter experiment
A quantitative study of the neutron environment in the Canfranc Underground
Laboratory has been performed. The analysis is based on a complete set of
simulations and, particularly, it is focused on the IGEX-DM dark matter
experiment. The simulations are compared to the IGEX-DM low energy data
obtained with different shielding conditions. The results of the study allow us
to conclude, with respect to the IGEX-DM background, that the main neutron
population, coming from radioactivity from the surrounding rock, is practically
eliminated after the implementation of a suitable neutron shielding. The
remaining neutron background (muon-induced neutrons in the shielding and in the
rock) is substantially below the present background level thanks to the muon
veto system. In addition, the present analysis gives us a further insight on
the effect of neutrons in other current and future experiments at the Canfranc
Underground Laboratory. The comparison of simulations with the body of data
available has allowed to set the flux of neutrons from radioactivity of the
Canfranc rock, (3.82 +- 0.44) x 10^{-6} cm^{-2} s^{-1}, as well as the flux of
muon-induced neutrons in the rock, (1.73 +- 0.22(stat) \+- 0.69(syst)) x
10^{-9} cm^{-2} s^{-1}, or the rate of neutron production by muons in the lead
shielding, (4.8 +- 0.6 (stat) +- 1.9 (syst)) x 10^{-9} cm^{-3} s^{-1}.Comment: 17 pages, 8 figures, elsart document class; final version to appear
in Astroparticle Physic
Status of the ANAIS Dark Matter Project at the Canfranc Underground Laboratory
The ANAIS experiment aims at the confirmation of the DAMA/LIBRA signal. A
detailed analysis of two NaI(Tl) crystals of 12.5 kg each grown by Alpha
Spectra will be shown: effective threshold at 1 keVee is at reach thanks to
outstanding light collection and robust PMT noise filtering protocols and the
measured background is well understood down to 3 keVee, having quantified K, U
and Th content and cosmogenic activation in the crystals. A new detector was
installed in Canfranc in March 2015 together with the two previous modules and
preliminary characterization results will be presented. Finally, the status and
expected sensitivity of the full experiment with 112 kg will be reviewed.Comment: Contributed to the 11th Patras Workshop on Axions, WIMPs and WISPs,
Zaragoza, June 22 to 26, 201
Production and relevance of cosmogenic radionuclides in NaI(Tl) crystals
The cosmogenic production of long-lived radioactive isotopes in materials is
an hazard for experiments demanding ultra-low background conditions. Although
NaI(Tl) scintillators have been used in this context for a long time, very few
activation data were available. We present results from two 12.5 kg NaI(Tl)
detectors, developed within the ANAIS project and installed at the Canfranc
Underground Laboratory. The prompt data taking starting made possible a
reliable quantification of production of some I, Te and Na isotopes with
half-lives larger than ten days. Initial activities underground were measured
and then production rates at sea level were estimated following the history of
detectors; a comparison of these rates with calculations using typical cosmic
neutron flux at sea level and a selected description of excitation functions
was also carried out. After including the contribution from the identified
cosmogenic products in the detector background model, we found that the
presence of 3H in the crystal bulk would help to fit much better our background
model and experimental data. We have analyzed the cosmogenic production of 3H
in NaI, and although precise quantification has not been attempted, we can
conclude that it could imply a very relevant contribution to the total
background below 15 keV in NaI detectors.Comment: Proceedings of the Low Radioactivity Techniques 2015 workshop, March
2015, Seattle (US
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