Transient Receptor Potential Ankyrin 1 (TRPA1) Methylation and Chronic Pain: A Systematic Review

Background and objective: Chronic pain represents a major global health issue in terms of psycho-physiological, therapeutic, and economic burden, not limited to adults but also to the pediatric age. Despite its great impact, its molecular mechanisms have still not been completely unraveled. Focusing on the impact of epigenetics in the pain complex trait, we assessed the association between chronic pain and the methylation pattern of TRPA1, a key gene related to pain sensitivity. Methods: We conducted a systematic review retrieving articles from three different databases. After deduplication, 431 items were subjected to manual screening, and then 61 articles were selected and screened again. Of these, only six were maintained for meta-analysis and analyzed using specific R packages. Results: Six articles were divided into two groups (group 1: comparison of mean methylation levels between healthy subjects and patients with chronic pain; group 2: correlation between mean methylation levels and pain sensation). A non-significant mean difference was obtained from the analysis of group 1 with a value of 3.97 (95% C.I. -7.79; 15.73). Analysis of group 2 showed a high level of variability between studies (correlation = 0.35, 95% C.I. -0.12; 0.82) due to their heterogeneity (I2 = 97%, p < 0.01). Conclusions: Despite the high variability observed in the different studies analyzed, our results suggest that hypermethylation and increased pain sensitivity could be connected, possibly due to the variation of TRPA1 expression

Las concepciones de profesoras de la enseñanza primaria sobre la prevención del uso indebido de drogas

Partiu-se da concepção de escola como lugar de formação de aprendizes na relação com educadores, privilegiado para trabalhar questões relevantes à vivência social, como o uso de drogas, desde o início da escolarização. Objetivou-se levantar concepções de professoras dos anos iniciais do ensino fundamental que participaram da quinta edição do curso de prevenção ao uso de drogas para educadores de escolas públicas sobre os conteúdos abordados e sua prática cotidiana. Buscou-se refletir sobre a importância de criar formas pedagógicas pautadas no acolhimento às vivências das crianças. Realizou-se entrevistas com vinte e uma professoras ex-cursistas do Distrito Federal. A análise das categorias construídas permitiu refletir sobre a prática educativa cotidiana e a necessidade da escuta dos discursos das crianças sobre as drogas. As professoras puderam perceber que há abertura para abordar essas questões e demandaram formação teórico-prática como instrumento que auxilie o trabalho pedagógico.This study is based on the conception of school as a place of education where apprentices establish a relation to educators and as privileged setting to work issues relevant to social life, such as drug use, since the beginning of schooling. The goal was to raise conceptions of elementary school teachers who participated in the fifth edition of the course for drug abuse prevention for public school educators, on the content covered and on their daily teaching practice. We sought to bethink the importance of creating educational ways guided by the embracement of children’s experiences. Interviews were conducted with twenty-one teachers from the Federal District who previously participated in the course. The analysis allowed thinking over everyday educational practices and the necessity of listening to children’s discourses about drugs. The teachers were able to realize that there is openness for addressing these issues and demanded theoretical and practical education as a tool to assist on their pedagogical work.La investigación partió de la concepción de escuela como espacio de formación de aprendices en relación con los educadores, privilegiado a trabajar cuestiones relevantes a la vida social, tales como el consumo de drogas, desde el inicio de la escolarización. Hubo como objetivo la búsqueda de las concepciones de profesoras de los años iníciales de la enseñanza primaria de escuelas públicas, participantes de la quinta edición del curso de prevención de abuso de drogas para educadores de la escuela pública, sobre los contenidos abordados en el curso y su práctica pedagógica diaria. Fueron entrevistadas veinte y una profesoras del Distrito Federal que hicieron el curso. El análisis de las categorías construidas permitió reflexionar sobre la práctica educativa cotidiana y la necesidad de escuchar los discursos de los niños sobre las drogas. Las profesoras fueron capaces de percibir que hay la posibilidad de abordar estas cuestiones y pidieron formación teórica y práctica como algo para ayudarlas en su trabajo

Transient Receptor Potential Ankyrin 1 (TRPA1) Methylation and Chronic Pain: A Systematic Review

Background and Objective: Chronic pain represents a major global health issue in terms of psycho-physiological, therapeutic, and economic burden, not limited to adults but also to the pediatric age. Despite its great impact, its molecular mechanisms have still not been completely unraveled. Focusing on the impact of epigenetics in the pain complex trait, we assessed the association between chronic pain and the methylation pattern of TRPA1, a key gene related to pain sensitivity. Methods: We conducted a systematic review retrieving articles from three different databases. After deduplication, 431 items were subjected to manual screening, and then 61 articles were selected and screened again. Of these, only six were maintained for meta-analysis and analyzed using specific R packages. Results: Six articles were divided into two groups (group 1: comparison of mean methylation levels between healthy subjects and patients with chronic pain; group 2: correlation between mean methylation levels and pain sensation). A non-significant mean difference was obtained from the analysis of group 1 with a value of 3.97 (95% C.I. &minus;7.79; 15.73). Analysis of group 2 showed a high level of variability between studies (correlation = 0.35, 95% C.I. &minus;0.12; 0.82) due to their heterogeneity (I2 = 97%, p &lt; 0.01). Conclusions: Despite the high variability observed in the different studies analyzed, our results suggest that hypermethylation and increased pain sensitivity could be connected, possibly due to the variation of TRPA1 expression

Reply to Piewbang et al. Domestic Cat Hepadnavirus Antigens in Lymphoma Tissues. Comment on Beatty et al. Domestic Cat Hepadnavirus and Lymphoma. Viruses 2023, 15, 2294.

We are grateful to the authors for providing additional data to demonstrate the presence of domestic cat hepadnavirus in lymphoma tissues [...]

Domestic Cat Hepadnavirus and Lymphoma.

We write to comment on Piewbang C et al [...]