441 research outputs found
Genetic Studies of Sulfadiazine-resistant and Methionine-requiring \u3cem\u3eNeisseria\u3c/em\u3e Isolated From Clinical Material
Deoxyribonucleate (DNA) preparations were extracted from Neisseria meningitidis (four isolates from spinal fluid and blood) and N. gonorrhoeae strains, all of which were resistant to sulfadiazine upon primary isolation. These DNA preparations, together with others from in vitro mutants of N. meningitidis and N. perflava, were examined in transformation tests by using as recipient a drug-susceptible strain of N. meningitidis (Ne 15 Sul-s Met+) which was able to grow in a methionine-free defined medium. The sulfadiazine resistance typical of each donor was introduced into the uniform constitution of this recipient. Production of p-aminobenzoic acid was not significantly altered thereby. Transformants elicited by DNA from the N. meningitidis clinical isolates were resistant to at least 200 μg of sulfadiazine/ml, and did not show a requirement for methionine (Sul-r Met+). DNA from six strains of N. gonorrhoeae, which were isolated during the period of therapeutic use of sulfonamides, conveyed lower degrees of resistance and, invariably, a concurrent methionine requirement (Sul-r/Met−). The requirement of these transformants, and that of in vitro mutants selected on sulfadiazine-agar, was satisfied by methionine, but not by vitamin B12, homocysteine, cystathionine, homoserine, or cysteine. Sul-r Met+ and Sul-r/Met− loci could coexist in the same genome, but were segregated during transformation. On the other hand, the dual Sul-r/Met− properties were not separated by recombination, but were eliminated together. DNA from various Sul-r/Met− clones tested against recipients having nonidentical Sul-r/Met− mutant sites yielded Sul-s Met+ transformants. The met locus involved is genetically complex, and will be a valuable tool for studies of genetic fine structure of members of Neisseria, and of genetic homology between species
Plurisubharmonic polynomials and bumping
We wish to study the problem of bumping outwards a pseudoconvex, finite-type
domain \Omega\subset C^n in such a way that pseudoconvexity is preserved and
such that the lowest possible orders of contact of the bumped domain with
bdy(\Omega), at the site of the bumping, are explicitly realised. Generally,
when \Omega\subset C^n, n\geq 3, the known methods lead to bumpings with high
orders of contact -- which are not explicitly known either -- at the site of
the bumping. Precise orders are known for h-extendible/semiregular domains.
This paper is motivated by certain families of non-semiregular domains in C^3.
These families are identified by the behaviour of the least-weight
plurisubharmonic polynomial in the Catlin normal form. Accordingly, we study
how to perturb certain homogeneous plurisubharmonic polynomials without
destroying plurisubharmonicity.Comment: 24 pages; corrected typos, fixed errors in Lemma 3.3; accepted for
publication in Math.
Metapopulation viability of an endangered shorebird depends on dispersal and human-created habitats: piping plovers (Charadrius melodus) and prairie rivers
Hermitian symmetric polynomials and CR complexity
Properties of Hermitian forms are used to investigate several natural
questions from CR Geometry. To each Hermitian symmetric polynomial we assign a
Hermitian form. We study how the signature pairs of two Hermitian forms behave
under the polynomial product. We show, except for three trivial cases, that
every signature pair can be obtained from the product of two indefinite forms.
We provide several new applications to the complexity theory of rational
mappings between hyperquadrics, including a stability result about the
existence of non-trivial rational mappings from a sphere to a hyperquadric with
a given signature pair.Comment: 19 pages, latex, fixed typos, to appear in Journal of Geometric
Analysi
Convergence of random zeros on complex manifolds
We show that the zeros of random sequences of Gaussian systems of polynomials
of increasing degree almost surely converge to the expected limit distribution
under very general hypotheses. In particular, the normalized distribution of
zeros of systems of m polynomials of degree N, orthonormalized on a regular
compact subset K of C^m, almost surely converge to the equilibrium measure on K
as the degree N goes to infinity.Comment: 16 page
Equidistribution of zeros of holomorphic sections in the non compact setting
We consider N-tensor powers of a positive Hermitian line bundle L over a
non-compact complex manifold X. In the compact case, B. Shiffman and S.
Zelditch proved that the zeros of random sections become asymptotically
uniformly distributed with respect to the natural measure coming from the
curvature of L, as N tends to infinity. Under certain boundedness assumptions
on the curvature of the canonical line bundle of X and on the Chern form of L
we prove a non-compact version of this result. We give various applications,
including the limiting distribution of zeros of cusp forms with respect to the
principal congruence subgroups of SL2(Z) and to the hyperbolic measure, the
higher dimensional case of arithmetic quotients and the case of orthogonal
polynomials with weights at infinity. We also give estimates for the speed of
convergence of the currents of integration on the zero-divisors.Comment: 25 pages; v.2 is a final update to agree with the published pape
Migratory shorebird adheres to Bergmann’s Rule by responding to environmental conditions through the annual lifecycle
The inverse relationship between body size and environmental temperature is a widespread ecogeographic pattern. However, the underlying forces that produce this pattern are unclear in many taxa. Expectations are particularly unclear for migratory species, as individuals may escape environmental extremes and reorient themselves along the environmental gradient. In addition, some aspects of body size are largely fixed while others are environmentally flexible and may vary seasonally. Here, we used a long-term dataset that tracked multiple populations of the migratory piping plover Charadrius melodus across their breeding and non-breeding ranges to investigate ecogeographic patterns of phenotypically flexible (body mass) and fixed (wing length) size traits in relation to latitude (Bergmann’s Rule), environmental temperature (heat conservation hypothesis), and migratory distance. We found that body mass was correlated with both latitude and temperature across the breeding and non-breeding ranges, which is consistent with predictions of Bergmann’s Rule and heat conservation. However, wing length was correlated with latitude and temperature only on the breeding range. This discrepancy resulted from low migratory connectivity across seasons and the tendency for individuals with longer wings to migrate farther than those with shorter wings. Ultimately, these results suggest that wing length may be driven more by conditions experienced during the breeding season or tradeoffs related to migration, whereas body mass is modified by environmental conditions experienced throughout the annual lifecycle
Migratory shorebird adheres to Bergmann’s Rule by responding to environmental conditions through the annual lifecycle
The inverse relationship between body size and environmental temperature is a widespread ecogeographic pattern. However, the underlying forces that produce this pattern are unclear in many taxa. Expectations are particularly unclear for migratory species, as individuals may escape environmental extremes and reorient themselves along the environmental gradient. In addition, some aspects of body size are largely fixed while others are environmentally flexible and may vary seasonally. Here, we used a long-term dataset that tracked multiple populations of the migratory piping plover Charadrius melodus across their breeding and non-breeding ranges to investigate ecogeographic patterns of phenotypically flexible (body mass) and fixed (wing length) size traits in relation to latitude (Bergmann’s Rule), environmental temperature (heat conservation hypothesis), and migratory distance. We found that body mass was correlated with both latitude and temperature across the breeding and non-breeding ranges, which is consistent with predictions of Bergmann’s Rule and heat conservation. However, wing length was correlated with latitude and temperature only on the breeding range. This discrepancy resulted from low migratory connectivity across seasons and the tendency for individuals with longer wings to migrate farther than those with shorter wings. Ultimately, these results suggest that wing length may be driven more by conditions experienced during the breeding season or tradeoffs related to migration, whereas body mass is modified by environmental conditions experienced throughout the annual lifecycle
Monitoring ecological change in UK woodlands and rivers: an exploration of the relational geographies of citizen science
The adoption of citizen science methodologies by environmental organisations and ecologists entrusts the task of collecting ecological data to non‐experts operating at a local scale. This presents individuals and communities with opportunities to monitor ecological change and contribute to local environmental management. Little is known about why volunteers choose to participate in burgeoning contemporary citizen science research initiatives. The aim of this paper is thus to explore volunteer motivation for involvement in two environmental citizen science initiatives, based in the United Kingdom. It contributes to understandings of the socio‐geographical influences that act on participation in environmental citizen science. It is proposed within this paper that affective connections with local geographies provide a conceptual framework for understanding citizen science motivations. The paper discusses the main themes emerging from site‐based, in‐depth interviews with 22 citizen science participants in various UK locations. The study revealed that early affective bonds formed with ecological spaces endured throughout life courses, while citizen science participation offered a way of remaining connected to local environments. The paper reflects on the endurance of affective environmental bonds and their manifestation within the expressed motivations for citizen science participation, which emerged as fulfilling a compulsion to observe ecological surroundings, a desire to participate in environmental research and a commitment to protecting local environments. The paper proposes that citizen science participation offers a framework to connect to and protect local and global affinity spaces, while assisting in monitoring global environmental change
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