Epigraphy, philology, and the hebrew bible : methodological perspectives on philological and comparative study of the hebrew bible in honor of Jo Ann Hackett

Introduction: "The present volume comprises a set of contradictions. It is simultaneously a Festschrift—usually conceived as a collection of essays honoring a colleague, teacher, and friend—and a volume designed with the graduate classroom in mind and organized around a few common themes. And whereas a few of the essays are typical exemplars of the genre of “introductory” or “overview” essay and reflecting engagement with the wider approaches to the disciplines at hand, many of the articles herein are specialized papers featuring a theoretical or methodological orientation appropriate to specific modes of study. This format, then, does not fit easily within any of the genres that are common within the fields of Biblical Studies and Northwest Semitic Philology. Yet, the constituent essays of this volume have been composed with two purposes: First, despite their eclectic and broadly-interested diversity of topics, these papers all attempt to grapple with specific problems associated with one of three topics that Professor Jo Ann Hackett has devoted her career to understanding: philological study of the Northwest Semitic languages; the study of epigraphic exemplars of those same languages; and the religious traditions of Israel and its neighbors in the Southern Levant, as reconstructed from the perspective(s) offered in the Hebrew Bible. Secondly, these articles are all oriented towards the educational context of graduate-level students of these same fields of study. These complementary goals are modeled on both the research and pedagogical work of Professor Hackett...

Caveolae optimize tissue factor-Factor VIIa inhibitory activity of cell-surface-associated tissue factor pathway inhibitor

TFPI (tissue factor pathway inhibitor) is an anticoagulant protein that prevents intravascular coagulation through inhibition of fXa (Factor Xa) and the TF (tissue factor)–fVIIa (Factor VIIa) complex. Localization of TFPI within caveolae enhances its anticoagulant activity. To define further how caveolae contribute to TFPI anticoagulant activity, CHO (Chinese-hamster ovary) cells were co-transfected with TF and membrane-associated TFPI targeted to either caveolae [TFPI–GPI (TFPI–glycosylphosphatidylinositol anchor chimaera)] or to bulk plasma membrane [TFPI–TM (TFPI–transmembrane anchor chimaera)]. Stable clones had equal expression of surface TF and TFPI. TX-114 cellular lysis confirmed localization of TFPI–GPI to detergent-insoluble membrane fractions, whereas TFPI–TM localized to the aqueous phase. TFPI–GPI and TFPI–TM were equally effective direct inhibitors of fXa in amidolytic assays. However, TFPI–GPI was a significantly better inhibitor of TF–fVIIa than TFPI–TM, as measured in both amidolytic and plasma-clotting assays. Disrupting caveolae by removing membrane cholesterol from EA.hy926 cells, which make TFPIα, CHO cells transfected with TFPIβ and HUVECs (human umbilical vein endothelial cells) did not affect their fXa inhibition, but significantly decreased their inhibition of TF–fVIIa. These studies confirm and quantify the enhanced anticoagulant activity of TFPI localized within caveolae, demonstrate that caveolae enhance the inhibitory activity of both TFPI isoforms and define the effect of caveolae as specifically enhancing the anti-TF activity of TFPI