Molecular biomarkers associated with respiratory insufficiency in amyotrophic lateral sclerosis

Abstract in proceedings of the Fourth International Congress of CiiEM: Health, Well-Being and Ageing in the 21st Century, held at Egas Moniz’ University Campus in Monte de Caparica, Almada, from 3–5 June 2019.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.info:eu-repo/semantics/publishedVersio

Methodologies and Applications Review

Funding Information: The Authors acknowledge Fundação para a Ciência e a Tecnologia (FCT-MCTES) for its financial support via the project UIDB/00667/2020 (UNIDEMI). Pedro M. Ferreira also acknowledges FCT-MCTES for funding the PhD grant UI/BD/151055/2021. Publisher Copyright: © 2022 by the authors.Sensing Technology (ST) plays a key role in Structural Health-Monitoring (SHM) systems. ST focuses on developing sensors, sensory systems, or smart materials that monitor a wide variety of materials’ properties aiming to create smart structures and smart materials, using Embedded Sensors (ESs), and enabling continuous and permanent measurements of their structural integrity. The integration of ESs is limited to the processing technology used to embed the sensor due to its high-temperature sensitivity and the possibility of damage during its insertion into the structure. In addition, the technological process selection is dependent on the base material’s composition, which comprises either metallic or composite parts. The selection of smart sensors or the technology underlying them is fundamental to the monitoring mode. This paper presents a critical review of the fundaments and applications of sensing technologies for SHM systems employing ESs, focusing on their actual developments and innovation, as well as analysing the challenges that these technologies present, in order to build a path that allows for a connected world through distributed measurement systems.publishersversionpublishe

Granting Sensorial Properties to Metal Parts through Friction Stir Processing

The authors would like also to thank to Micronsense-Metrologia Industrial (Leiria, Portugal) for the μCT analysis. The authors would also like to thank Prof. Catarina Santos for granting access to the MicroLab - Electron Microscopy Laboratory (Instituto Superior Técnico) for the SEM analyses.Structural Health Monitoring systems assess the part's current condition. This can be performed with a monitoring system comprising sensors, on the surface or embedded, in the monitored parts. However, surface sensors are subject to damage, and embedding the sensors may result in a weakened part. An innovative Self-Sensing Material and its manufacturing process were developed and are presented herein. As proof of concept, Barium Titanate particles were introduced and dispersed into an AA5083-H111 part by Friction Stir Processing (FSP). The particles’ distribution and concentration was evaluated by a set of characterization techniques, demonstrating that greater concentrations, grant enhanced sensitivity to the material. The use of FSP and the embedded particles improved the part’s mechanical behaviour in the processed zone. The sensorial properties were assessed and the response to a set of dynamic loads was measured, being coherent with the solicitations provided. The developed self-sensing material revealed an electrical sensitivity of 12.0 × 10-4 uV/MPa.publishersversionpublishe

A Case Study

Funding Information: Inês A. Ferreira acknowledges the financial support from Fundação para a Ciência e Tecnologia (FCT) for funding PhD Grant-REF: SFRH/BD/145448/2019. Helena Carvalho acknowledges Fundação para a Ciência e Tecnologia (FCT) for its financial support through the project UIDB/00667/2020 (UNIDEMI). Carina Pimentel acknowledges Fundação para a Ciência e Tecnologia (FCT) within the R&D Units Project Scope UIDB/00319/2020. Publisher Copyright: © 2023 by the authors.The number of variants of the vehicle routing problem (VRP) has grown rapidly in the last decades. Among these, VRPs with time window constraints are among the most studied ones. However, the literature regarding VRPs that concerns the delivery and installation of products is scarce. The main aim of this study was to propose a heuristic approach for the route planning process of a company whose focus is on furniture delivery and assembly and, thus, contributing to the research around the Delivery and Installation Routing Problem. The case study method was used, and two scenarios were compared: the current scenario (showing the routes created by the company worker); and the future scenario (representing the routes created by the heuristic). Results show that the proposed heuristic approach provided a feasible solution to the problem, allowing it to affect customers and teams without compromising the teams’ competencies and respecting all constraints.publishersversionpublishe

Hole-in-One Mutant Phenotypes Link EGFR/ERK Signaling to Epithelial Tissue Repair in Drosophila

Background: Epithelia act as physical barriers protecting living organisms and their organs from the surrounding environment. Simple epithelial tissues have the capacity to efficiently repair wounds through a resealing mechanism. The known molecular mechanisms underlying this process appear to be conserved in both vertebrates and invertebrates, namely the involvement of the transcription factors Grainy head (Grh) and Fos. In Drosophila, Grh and Fos lead to the activation of wound response genes required for epithelial repair. ERK is upstream of this pathway and known to be one of the first kinases to be activated upon wounding. However, it is still unclear how ERK activation contributes to a proper wound response and which molecular mechanisms regulate its activation. Methodology/Principal Findings: In a previous screen, we isolated mutants with defects in wound healing. Here, we describe the role of one of these genes, hole-in-one (holn1), in the wound healing process. Holn1 is a GYF domain containing protein that we found to be required for the activation of several Grh and Fos regulated wound response genes at the wound site. We also provide evidence suggesting that Holn1 may be involved in the Ras/ERK signaling pathway, by acting downstream of ERK. Finally, we show that wound healing requires the function of EGFR and ERK signaling. Conclusions/Significance: Based on these data, we conclude that holn1 is a novel gene required for a proper wound healing response. We further propose and discuss a model whereby Holn1 acts downstream of EGFR and ERK signaling i

Determinant Factors of the Organizational Innovative Performance: Cooperation, Absorptive Capacity and Public Policy

This paper aims to analyze the determinants of the innovative performance of the Portuguese firms which, in the context of this research are: cooperation, public policies to stimulate innovation and absorptive capacity. Indeed, the set of approaches that have come to appear as a reference of the subject of innovation are assumed of conceptual importance, leading to an empirical basis that allows to analyze the determinants of innovative performance, using the available data from the Community Innovation Survey CIS 2010 and the application of statistical patterns, through logistic regression modelsinfo:eu-repo/semantics/publishedVersio

Barriers to innovation activities as determinants of ongoing activities or abandoned

Purpose – This paper aims to analyses barriers as a determinant of ongoing or abandoned innovation activities. The literature exploits barriers as the nature of innovation and its influence on firm’s. The main focus is the Portuguese SMEs and the impacts that act as barriers in the development of innovation activities. Design/methodology/approach – The database was obtained through the Community Innovation Survey 2010 (CIS 2010) that was coordinated by EUROSTAT. In this sense, a logistic regression model is proposed, which makes it possible to analyse the relations between three or more variables, depending on whether the relationship is one of dependence or interdependence, thus allowing the application of distinct statistical techniques, using 6,160 firms Findings – There are several sources of information that are associated with the development of innovation projects. Practical implications – The importance of barriers to the development of innovation activities, as well as it being a decisive factor in the impediment to and abandon of the same, was noted. Originality/value – This study also demonstrated that each case is different and that a barrier in one firm can at the same time be a window of opportunity for another firm.info:eu-repo/semantics/publishedVersio

2,3-Diphosphoglycerate and the Protective Effect of Pyruvate Kinase Deficiency against Malaria Infection—Exploring the Role of the Red Blood Cell Membrane

Malaria remains a major world public health problem, contributing to poverty and inequality. It is urgent to find new efficacious tools with few adverse effects. Malaria has selected red blood cell (RBC) alterations linked to resistance against infection, and understanding the protective mechanisms involved may be useful for developing host-directed tools to control Plasmodium infection. Pyruvate kinase deficiency has been associated with resistance to malaria. Pyruvate kinase-deficient RBCs display an increased concentration of 2,3-diphosphoglycerate (2,3-DPG).We recently showed that 2,3-DPG impacts in vitro intraerythrocytic parasite growth, induces a shift of the metabolic profile of infected cells (iRBCs), making it closer to that of noninfected ones (niRBCs), and decreases the number of parasite progenies that invade new RBCs. As an increase of 2,3-DPG content may also have an adverse effect on RBC membrane and, consequently, on the parasite invasion, in this study, we explored modifications of the RBC morphology, biomechanical properties, and RBC membrane on Plasmodium falciparum in vitro cultures treated with 2,3-DPG, using atomic force microscopy (AFM)-based force spectroscopy and other experimental approaches. The presence of infection by P. falciparum significantly increased the rigidity of parasitized cells and influenced the morphology of RBCs, as parasitized cells showed a decrease of the area-to-volume ratio. The extracellular addition of 2,3-DPG also slightly affected the stiffness of niRBCs, making it more similar to that of infected cells. It also changed the niRBC height, making the cells appear more elongated. Moreover, 2,3-DPG treatment influenced the cell surface charge, becoming more negative in treated RBCs than in untreated ones. The results indicate that treatment with 2,3-DPG has only a mild effect on RBCs in comparison with the effect of the presence of the parasite on the host cell. 2,3-DPG is an endogenous host metabolite, which may, in the future, originate a new antimalarial tool with few adverse effects on noninfected cells.publishersversionpublishe

Pathologic expansion in the C9orf72 gene is associated with accelerated decline of respiratory function and decreased survival in amyotrophic lateral sclerosis

Abstract in proceedings of the Fourth International Congress of CiiEM: Health, Well-Being and Ageing in the 21st Century, held at Egas Moniz’ University Campus in Monte de Caparica, Almada, from 3–5 June 2019.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.info:eu-repo/semantics/publishedVersio