Flexible control of movement in plants

Although plants are essentially sessile in nature, these organisms are very much in tune with their environment and are capable of a variety of movements. This may come as a surprise to many non-botanists, but not to Charles Darwin, who reported that plants do produce movements. Following Darwin\u2019s specific interest on climbing plants, this paper will focus on the attachment mechanisms by the tendrils. We draw attention to an unsolved problem in available literature: whether during the approach phase the tendrils of climbing plants consider the structure of the support they intend to grasp and plan the movement accordingly ahead of time. Here we report the first empirical evidence that this might be the case. The three-dimensional (3D) kinematic analysis of a climbing plant (Pisum sativum L.) demonstrates that the plant not only perceives the support, but it scales the kinematics of tendrils\u2019 aperture according to its thickness. When the same support is represented in two-dimensions (2D), and thus unclimbable, there is no evidence for such scaling. In these circumstances the tendrils\u2019 kinematics resemble those observed for the condition in which no support was offered. We discuss these data in light of the evidence suggesting that plants are equipped with sensory mechanisms able to provide the necessary information to plan and control a movement

VST - VLT Survey Telescope Integration Status

The VLT Survey Telescope (VST) is a 2.6m aperture, wide field, UV to I facility, to be installed at the European Southern Observatory (ESO) on the Cerro Paranal Chile. VST was primarily intended to complement the observing capabilities of VLT with wide-angle imaging for detecting and pre-characterising sources for further observations with the VLT.Comment: 2 pages, 2 figures, conferenc

15 kDa Granulysin versus GM-CSF for monocytes differentiation: analogies and differences at the transcriptome level

<p>Abstract</p> <p>Background</p> <p>Granulysin is an antimicrobial and proinflammatory protein with several isoforms. While the 9 kDa isoform is a well described cytolytic molecule with pro-inflammatory activity, the functions of the 15 kDa isoform is less well understood. Recently it was shown that 15 kDa Granulysin can act as an alarmin that is able to activate monocytes and immature dendritic cells. Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) is a growth factor widely used in immunotherapy both for <it>in vivo </it>and <it>ex vivo </it>applications, especially for its proliferative effects.</p> <p>Methods</p> <p>We analyzed gene expression profiles of monocytes cultured with 15 kDa Granulysin or GM-CSF for 4, 12, 24 and 48 hours to unravel both similarities and differences between the effects of these stimulators.</p> <p>Results</p> <p>The analysis revealed a common signature induced by both factors at each time point, but over time, a more specific signature for each factor became evident. At all time points, 15 kDa Granulysin induced immune response, chemotaxis and cell adhesion genes. In addition, only 15 kDa Granulsyin induced the activation of pathways related to fundamental dendritic cell functions, such as co-stimulation of T-cell activation and Th1 development. GM-CSF specifically down-regulated genes related to cell cycle arrest and the immune response. More specifically, cytokine production, lymphocyte mediated immunity and humoral immune response were down-regulated at late time points.</p> <p>Conclusion</p> <p>This study provides important insights on the effects of a novel agent, 15 kDa granulysin, that holds promise for therapeutic applications aimed at the activation of the immune response.</p

The Vallo di Diano Range-Bounding Fault-System (Southern Italy): New Evidence of Recent Activity From High-Resolution Seismic Profiling

The Vallo di Diano is the largest intermountain basin in the Southern Apennines (Italy). The basin evolution was controlled by the Quaternary activity of a range-bounding, SW-dipping normal fault system located to the east (Vallo di Diano Fault System, VDFS). Geological and oil industry data define the sin-sedimentary activity of the VDFS up to the Middle Pleistocene. However, commercial profiles do not resolve the shallower, eastern portion of the basin, due to strong lateral heterogeneities and unfavourable surface conditions. Therefore, Late Pleistocene-Holocene activity of the VDFS and its seismogenic potential are still uncertain. To better constrain the shallow structure of the basin, we performed four high-resolution seismic surveys, along its eastern side, where slope breccias and fans cover the Mesozoic carbonate bedrock and bury the VDFS. We also investigated some NW-trending flexures affecting Late Pleistocene fans, that we had previously detected and dubitatively ascribed to recent faulting. Seismic data were acquired with a dense wide-aperture geometry. Two high-resolution (HR) NE-trending profiles, about 1.5 km long, were collected using respectively 5 m and 10 m spaced receivers and sources. Two very high-resolution (VHR) NE-trending profiles, 400 and 350 m long, with densely spaced sources (4 m) and receivers (2 m) were also collected. HR profiling was aimed at imaging alluvial fan thickness and morphology of the underlying carbonate bedrock. VHR surveys targeted the flexures and their possible origin. All lines were acquired with a HR vibroseis source, except for the shortest profile, where we used a buffalo-gun, better suited for very near-surface imaging (z < 50 m depth). Seismic imaging consists of reflectivity images obtained by CDP-processing of reflection data complemented by Vp images obtained by multi-scale seismic tomography. The stack sections illuminated the basin down to 0.4-0.5 s TWT and reveal an array of high-angle, generally SW-dipping faults dissecting the bedrock and the alluvial fans. Faulting created accommodation space in the hanging-wall and displaced the different fan generations. Clear reflection truncations in the stack-sections correspond to significant Vp lateral changes in the tomographic images. VHR tomography is well defined along the shortest line down to 40 m depth, where two steps within slope breccias are visible. Moreover, two low-velocity wedges (colluvial packages) are imaged in the near surface (5-20 m depth). These data support recent faulting consistently with surface geomorphic features. We interpret these fault structures as splays of the range bounding master fault. Comparison with commercial reflection profiles nearby reveals a great improvement in seismic imaging achieved by HR surveys, which allow a detailed seismostratigraphic analysis of the basin.UnpublishedSan Francisco (CA), Moscone West, Howard Street3.2. Tettonica attivaope

The Vallo di Diano Range-Bounding Fault-System (Southern Italy): New Evidence of Recent Activity From High-Resolution Seismic Profiling

The Vallo di Diano is the largest intermountain basin in the Southern Apennines (Italy). The basin evolution was controlled by the Quaternary activity of a range-bounding, SW-dipping normal fault system located to the east (Vallo di Diano Fault System, VDFS). Geological and oil industry data define the sin-sedimentary activity of the VDFS up to the Middle Pleistocene. However, commercial profiles do not resolve the shallower, eastern portion of the basin, due to strong lateral heterogeneities and unfavourable surface conditions. Therefore, Late Pleistocene-Holocene activity of the VDFS and its seismogenic potential are still uncertain. To better constrain the shallow structure of the basin, we performed four high-resolution seismic surveys, along its eastern side, where slope breccias and fans cover the Mesozoic carbonate bedrock and bury the VDFS. We also investigated some NW-trending flexures affecting Late Pleistocene fans, that we had previously detected and dubitatively ascribed to recent faulting. Seismic data were acquired with a dense wide-aperture geometry. Two high-resolution (HR) NE-trending profiles, about 1.5 km long, were collected using respectively 5 m and 10 m spaced receivers and sources. Two very high-resolution (VHR) NE-trending profiles, 400 and 350 m long, with densely spaced sources (4 m) and receivers (2 m) were also collected. HR profiling was aimed at imaging alluvial fan thickness and morphology of the underlying carbonate bedrock. VHR surveys targeted the flexures and their possible origin. All lines were acquired with a HR vibroseis source, except for the shortest profile, where we used a buffalo-gun, better suited for very near-surface imaging (z < 50 m depth). Seismic imaging consists of reflectivity images obtained by CDP-processing of reflection data complemented by Vp images obtained by multi-scale seismic tomography. The stack sections illuminated the basin down to 0.4-0.5 s TWT and reveal an array of high-angle, generally SW-dipping faults dissecting the bedrock and the alluvial fans. Faulting created accommodation space in the hanging-wall and displaced the different fan generations. Clear reflection truncations in the stack-sections correspond to significant Vp lateral changes in the tomographic images. VHR tomography is well defined along the shortest line down to 40 m depth, where two steps within slope breccias are visible. Moreover, two low-velocity wedges (colluvial packages) are imaged in the near surface (5-20 m depth). These data support recent faulting consistently with surface geomorphic features. We interpret these fault structures as splays of the range bounding master fault. Comparison with commercial reflection profiles nearby reveals a great improvement in seismic imaging achieved by HR surveys, which allow a detailed seismostratigraphic analysis of the basin

Exposure to perfluoroalkyl substances (PFAS) and association with thyroid hormones in adolescent males

Background: Perfluoroalkyl substances (PFAS) are found in a wide range of consumer products. Exposure to PFAS in children and adolescents may be associated with alterations in thyroid hormones, which have critical roles in brain function. Objective: This study investigated the association between plasma concentrations of PFAS and serum levels of total triiodothyronine (T3), free thyroxine (T4), and thyroid-stimulating hormone (TSH) in adolescent males. Methods: In 2017–2019, 151 boys from the Environment and Childhood (INMA)-Granada birth cohort, Spain, participated in a clinical follow up visit at the age of 15–17 years. Plasma concentrations of ten PFAS (PFHxA, PFHpA, PFOA, PFNA, PFDA, PFUnDA, PFDoDA, PFTrDA, PFOS, and PFHxS) and serum thyroid hormones were measured in 129 of these boys. Linear regression analysis was performed to determine associations of individual PFAS with total T3, free T4, TSH, and free T4/TSH ratio, and quantile g-computation models were performed to assess the mixture effect. Additional models considered iodine status as effect modifier. Results: PFOS was the most abundant PFAS in plasma (median = 2.22 μg/L), followed by PFOA (median = 1.00 μg/L), PFNA (median = 0.41 μg/L), and PFHxS (median = 0.40 μg/L). When adjusted by confounders (including age, maternal schooling, and fish intake), PFOA and PFUnDA were associated with an increase in free T4 (β [95% CI] = 0.72 [0.06; 1.38] and 0.36 [0.04; 0.68] pmol/L, respectively, per two-fold increase in plasma concentrations), with no change in TSH. PFOS, the sum of PFOA, PFNA, PFOS, and PFHxS, and the sum of long-chain PFAS were marginally associated with increases in free T4. Associations with higher free T4 and/or total T3 were seen for several PFAS in boys with lower iodine intake (<108 μ/day) alone. Moreover, the PFAS mixture was association with an increase in free T4 levels in boys with lower iodine intake (% change [95% CI] = 6.47 [–0.69; 14.11] per each quartile increase in the mixture concentration). Conclusions: Exposure to PFAS, considered individually or as a mixture, was associated with an increase in free T4 levels in boys with lower iodine intake. However, given the small sample size, the extent of these alterations remains uncertainSpanish Consortium for Research on Epidemiology and Public Health (CIBERESP)Instituto de Salud Carlos III (ISCIII) (grant no. CP16/00085

Intra-subject variability in human bone marrow stromal cell (BMSC) replicative senescence: Molecular changes associated with BMSC senescence

AbstractThe outcomes of clinical trials using bone marrow stromal cell (BMSC) are variable; the degree of the expansion of BMSCs during clinical manufacturing may contribute to this variability since cell expansion is limited by senescence. Human BMSCs from aspirates of healthy subjects were subcultured serially until cell growth stopped. Phenotype and functional measurements of BMSCs from two subjects including senescence-associated beta-galactosidase staining and colony formation efficiency changed from an early to a senescence pattern at passage 6 or 7. Transcriptome analysis of 10 early and 15 late passage BMSC samples from 5 subjects revealed 2122 differentially expressed genes, which were associated with immune response, development, and cell proliferation pathways. Analysis of 57 serial BMSC samples from 7 donors revealed that the change from an early to senescent profile was variable among subjects and occurred prior to changes in phenotypes. BMSC age expressed as a percentage of maximum population doublings (PDs) was a good indicator for an early or senescence transcription signature but this measure of BMSC life span can only be calculated after expanding BMSCs to senescence. In order to find a more useful surrogate measure of BMSC age, we used a computational biology approach to identify a set of genes whose expression at each passage would predict elapsed age of BMSCs. A total of 155 genes were highly correlated with BMSC age. A least angle regression algorithm identified a set of 24 BMSC age-predictive genes. In conclusion, the onset of senescence-associated molecular changes was variable and preceded changes in other indicators of BMSC quality and senescence. The 24 BMSC age predictive genes will be useful in assessing the quality of clinical BMSC products

Feasibility and reproducibility of right ventricle stress echocardiography and its capability to assess the right ventricle contractile reserve of patient with at least trivial tricuspid regurgitation

Abstract Funding Acknowledgements Type of funding sources: None. BACKGROUND. Stress echocardiography (SE) is widely used for the assessment of left ventricular (LV) function, diagnostic and prognostic stratification of patients with coronary artery disease and for assessment of mitral and aortic valve disease. However, the assessment of the right ventricle (RV) in general, and in particular in regard to the contractile reserve of the RV in patients with tricuspid valve (TV) disease is an area that has not been previously explored in adult patients. The physiology and function of the RV is different than that of the LV and the use of SE provides the possibility to test both systolic and diastolic function of the RV in response to increased loading conditions. This can potentially be used to assess the RV function prior to surgery and to predict which subset of patients may benefit from intervention on the TV before the RV displays signs of failure PURPOSE. We therefore propose a study to investigate the potential use of SE for the assessment of RV function in adult patients. The aim is to evaluate the feasibility of RV SE in any patients with more than trivial tricuspid regurgitation (TR) and to assess the presence and degree of RV contractile reserve. METHODS. We enrolled 81 patients undergoing a phisical or dobutamine SE for CV risk stratification or chest pain. Inclusion criteria were age≥ 18 years, normal baseline RV function (FAC&gt; 35%, TAPSE&gt; 16 mm). Exclusion criteria were presence of RV dysfunction, pulmonary stress hypertension, positive stress test for left myocardial ischemia, presence of moderate or severe valvular disease, grade III or higher diastolic dysfunction at baseline, severe respiratory, renal or hepatic dysfunction. We evaluated the average values of TAPSE, fractional area change (FAC), S wave, sPAP (pulmonary systolic blood pressure), RV strain during baseline and at the peak of the effort. We also assessed the reproducibility of these measurement between two different expert operators (blind analysis). RESULTS. We were able to measure the RV parameters both during baseline and at the peak of the effort in all patients, demonstrating an excellent feasibility. Differences in parameters collected at baseline and at peak were assessed using paired Wilcoxon signed rank test. All variables showed a statistical significant increase (p &lt; 0.001) at peak compared to the baseline. Average percentage increases at peak were 31.1% for TAPSE, 24,8% for FAC, 50,6% for S wave, 55,2% for PAPS and 39.8 % for RV strain. Bland-Altman method was used to evaluate the agreement between measurements collected by two separate operators and it showed good Intraclass Correlation Coefficients (Figure). CONCLUSIONS. RV SE proved to be feasible and showed little inter-operator variability in patients with at least trivial TR. It provided valuable informations about RV contractile reserve that may help stratifying the risk of RV failure in patients undergoing TV surgery. Abstract Figur

Trying to Grasp a Sketch of a Brain for Grasping

Ritter H, Haschke R, Steil JJ. Trying to Grasp a Sketch of a Brain for Grasping. In: Sendhoff B, ed. Creating Brain-Like Intelligence. Lecture Notes in Artificial Intelligence; 5436. Berlin, Heidelberg: Springer; 2009: 84-102