Changes in the fecal microbiota associated with a broad‐spectrum antimicrobial administration in hospitalized neonatal foals with probiotics supplementation

There is a wide array of evidence across species that exposure to antibiotics is associated with dysbiosis, and due to their widespread use, this also raises concerns also in medicine. The study aimed to determine the changes on the fecal microbiota in hospitalized neonatal foals administered with broad‐spectrum antimicrobials and supplemented probiotics. Fecal samples were collected at hospital admission (Ta), at the end of the antimicrobial treatment (Te) and at discharge (Td). Feces were analysed by next‐generation sequencing of the 16S rRNA gene on Illumina MiSeq. Seven foals treated with IV ampicillin and amikacin/gentamicin were included. The mean age at Ta was 19 h, the mean treatment length was 7 days and the mean time between Te and Td was 4.3 days. Seven phyla were identified: Actinobacteria, Bacteroidetes, Firmicutes, Fusobacteria, Proteobacteria, TM7 and Verrucomicrobia. At Ta, Firmicutes (48.19%) and Proteobacteria (31.56%) were dominant. The alpha diversity decreased from Ta to Te, but it was the highest at Td. The beta diversity was higher at Ta than at Te and higher at Td than at Te. An increase in Akkermansia over time was detected. The results suggest that the intestinal microbiota of neonatal foals rapidly returns to a high diversity after treatment. It is possible that in foals, the effect of antimicrobials is strongly influenced or overshadowed by the time‐dependent changes in the developing gut microbiota

Searching for the Least Invasive Management of Pelvi-Ureteric Junction Obstruction in Children: A Critical Literature Review of Comparative Outcomes

Introduction: To review the published evidence on the minimally invasive pyeloplasty techniques available currently with particular emphasis on the comparative data about the various minimally invasive alternatives to treat pelvi-ureteric junction obstruction and gauge if one should be favored under certain circumstances. Materials and Methods: Non-systematic review of literature on open and minimally invasive pyeloplasty including various kinds of laparoscopic procedures, the robotic-assisted laparoscopic pyeloplasty, and endourological procedures. Results: Any particular minimally invasive pyeloplasty procedure seems feasible in experienced hands, irrespective of age including infants. Comparative data suggest that the robotic-assisted procedure has gained wider acceptance mainly because it is ergonomically more suited to surgeon well-being and facilitates advanced skills with dexterity thanks to 7 degrees of freedom. However, costs remain the major drawback of robotic surgery. In young children and infants, instead, open surgery can be performed via a relatively small incision and quicker time frame. Conclusions: The best approach for pyeloplasty is still a matter of debate. The robotic approach has gained increasing acceptance over the last years with major advantages of the surgeon well-being and ergonomics and the ease of suturing. Evidence, however, may favor the use of open surgery in infancy

In situ immunofluorescent staining of autophagy in muscle stem cells

Increasing evidence points to autophagy as a crucial regulatory process to preserve tissue homeostasis. It is known that autophagy is involved in skeletal muscle development and regeneration, and the autophagic process has been described in several muscular pathologies and agerelated muscle disorders. A recently described block of the autophagic process that correlates with the functional exhaustion of satellite cells during muscle repair supports the notion that active autophagy is coupled with productive muscle regeneration. These data uncover the crucial role of autophagy in satellite cell activation during muscle regeneration in both normal and pathological conditions, such as muscular dystrophies. Here, we provide a protocol to monitor the autophagic process in the adult Muscle Stem Cell (MuSC) compartment during muscle regenerative conditions. This protocol describes the setup methodology to perform in situ immunofluorescence imaging of LC3, an autophagy marker, and MyoD, a myogenic lineage marker, in muscle tissue sections from control and injured mice. The methodology reported allows for monitoring the autophagic process in one specific cell compartment, the MuSC compartment, which plays a central role in orchestrating muscle regeneration

Adjustment and transformation strategies of ItalPoS Permanent GNSS Network

A private network of 133 Global Navigation Satellite Systems (GNSS) permanent sites, named ItalPoS (Italian Positioning Service) and uniformly covering the entire Italian territory, was established in April 2006 by the Italian Division of Leica Geosystems S.p.A. This network also involves several GPS stations of the INGV (Italian National Institute of Geophysics and Volcanology) RING (Real-time Integrated National Gps) network and GPS stations from other public and private bodies. Indeed Leica Geosystems contributed with its experience and its products to these networks which currently fulfil the national coverage where there are no ItalPoS stations. For instance, the purpose of the ItalPoS network is mainly GNSS data distribution in terms of real time corrections and RINEX files for the post-processing. In order to compute and distribute to private and scientific users a set of adjusted coordinates of the ItalPoS network in the IGS05 and ETRF89-2000 reference frames, a dataset of RINEX of about 120 GPS permanent sites was created and processed, using the distributed sessions approach, by means of Gamit/Globk 10.3 GPS data processor, also using some SOPAC (Scripps Orbits and Permanent Array Center) and EUREF (European Permanent Network) solutions to improve the reference frame definition and the internal constraints. This paper describes all the strategies adopted to estimate coordinates and velocities of the ItalPoS Network in IGS05 and in ETRF89-2000 reference frames in the whole Italian territory

Un approccio multidisciplinare per documentare e visualizzare un sito archeologico: la villa d’otium di Massaciuccoli (Massarosa, LU)

The research project VisualVersilia 3D aims at offering a new method to record andvisualize the territory and its heritage by matching the traditional reading of documents and the potential use of modern communication technologies. The purpose of the project is to define a methodology that can be applied to material culture, as well as to other types of contents and contexts, to enhance the characteristic features of the territory and its heritage. The innovation of the project consists in the development of a procedure for documenting current and past historical times and integrating their 3D visualizations with rendering, capable of returning an immersive virtual reality for a successful enhancement of the heritage. The research implements the methodology in the archaeological complex of Massaciuccoli (Massarosa, LU), one of the best preserved Roman site of the Versilia area (Tuscany, Italy). The activities briefly consist in: 1) analysis of all types of available sources; 2) metric three-dimensional survey by laser scanning technology addressed to the structures and buildings of the complex; 3) laser scanning data processing; 4) realization of virtual 3D rendering related to Roman and current condition for documentation and conservation purposes; 5) creation of virtual tour of the site in its current form, on the basis of spherical images then enhanced by texts, and 3D models of the Roman age

Understanding the gender wage gap differential between public and private sector in Italy : a quantile approach for panel data

This paper analyzes the gender wage gaps across the wage distribution in both the private and public sectors in Italy for the years 2005-2010. We use quantile regression methods to estimate and decompose the gender wage gap at different points of the wage distribution. We find in both sectors a consistent level of gender wage gap (lower in the public sector) and an increasing path along the wage distribution. Counterfactual decomposition analysis supports the idea of a sticky floor mechanism in action in the private sector and of a glass ceiling in the public sector. In addition to standard decomposition techniques we propose a two step procedure that relies on a novel approach to estimating fixed effects quantile regressions. Its main advantage is that it allows the estimation of the marginal effect of the employment sector on wages at different points of the distribution, while accounting for both observable and time-invariant unobservable factors. When we control for employees\u2019 observed and unobservable individual characteristics, the main finding is that the gender wage gap substantially decreases in both sectors. A second evidence is that the sticky floor effect in the private sector vanishes, while the glass ceiling effect in the public sector remains. The evidence from the longitudinal analysis amplifies the differences of the wage-setting mechanisms in the two sectors

Comparative study of imiquimod 3.75% vs. photodynamic therapy for actinic keratosis of the scalp

Background/purpose: To assess efficacy, tolerability, adverse effects, recurrence, and aesthetic results of imiquimod 3.75% vs. photodynamic therapy with 5-aminolaevulinic acid (MAL-PDT) for actinic keratosis (AK). Methods: A small randomized, intraindividual right-left pilot study for AK treatment of multiple scalp lesions was performed. Patients were treated with imiquimod and subsequently MAL-PDT (on opposite sides of the scalp) 14 days apart. Study end points were evaluated with clinical and dermoscopic examinations at 1, 3, 6, and 12 months. Results: Nine male bald patients were enrolled. Imiquimod achieved a slightly higher overall clearance rate than MAP-PDT (68.1% vs 56.5%). According to AK degree of severity, clearance rates were greater for degree I and III with imiquimod (68.8%, 64.5% and 75% with imiquimod vs. 48%, 69.8%, and 66.7% for MAL-PDT, respectively). At 12 months, a slightly higher total recurrence rate was noted for imiquimod compared with MAL-PDT (9.9% vs. 8.6%); new lesions were 2 degree I for imiquimod and 4 degree I for MAL-PDT. For both treatments, pain was moderate/strong (even if MAL-PDT seems to be less tolerable) adverse effects are common and transient; aesthetic results excellent. Conclusion: Both imiquimod and MAL-PDT were effective in the reduction in the number of AK. In the long-term, both present a good effectiveness maintained over time with excellent aesthetic results. A combination or sequential therapy could optimize the management of the cancerization field