2,305 research outputs found
A Rare Presentation of Invasive Tuberculosis of the Central Nervous System in an Immunocompetent Patient in a Nonendemic Country.
We herein report a rare case of a 25-year-old immunocompetent male patient with disseminated tuberculosis of central nervous system (CNS), first presenting as multiple cerebral lesions with no meningeal involvement. Subsequent diagnostic workup disclosed extensive peritoneal involvement. A broad differential diagnosis was considered, including neoplastic and infectious diseases. The diagnosis was confirmed with positive PCR result for Mycobacterium tuberculosis in the biopsied mesenteric tissue. The patient was started on tuberculostatic regimen with favorable outcome. No acquired or hereditary immunodeficiency was documented. Disseminated tuberculosis in immunocompetent individuals is extremely rare. Genetic susceptibility factors have been reported in individuals with extensive forms of the disease and a high index of suspicion is required, as observed in our case.info:eu-repo/semantics/publishedVersio
Demencia rápidamente progresiva en adulto joven como forma de presentación de panencefalitis esclerosante subaguda
info:eu-repo/semantics/publishedVersio
Indicadores agrÃcolas das lavouras de subsistência financiadas pelo PRONAF no estado do Ceará.
Resumo: O objetivo deste estudo foi analisar a influência do PRONAF-Programa Nacional de Fortalecimento da Agricultura Familiar sobre os indicadores agrÃcolas: produção, área plantada e produtividade das lavouras de subsistências beneficiadas pelo Programa no Estado do Ceará, no perÃodo de 1999 a 2005. A pesquisa aplicou análises tabular e gráfica para estudar o comportamento das variáveis. Os resultados registraram que as lavouras de subsistência mais beneficiadas pelo Programa foram: algodão, arroz, cana-de-açúcar, feijão, mandioca e milho. Uma comparação destas lavouras com outras não beneficiadas: banana, castanha de caju e mamona revelou que apesar do financiamento as lavouras beneficiadas pelo PRONAF obtiveram um desempenho inferior ao das não beneficiadas em relação aos indicadores agrÃcolas analisados. Concluiu-se que a influência do PRONAF no desempenho dos indicadores agrÃcolas das lavouras de subsistência é muito pequena. Abstract: The objective of this study was to analyze influence the PRONAF-National Program of Fortifies of Familiar Agriculture on the agricultural pointers: production, planted area and productivity of subsistences the farmings benefited for the Program in the State of the Ceará, the period 1999 to 2005. The research applied tabulate analyses and graphical. The subsistences of farmings more benefited by the Program had been: cotton, rice, sugar cane-of-sugar, beans, cassava and maize. A comparison with others benefited not of these farmings: banana, chestnut and mamona disclosed that despite benefited the farmings financing for the PRONAF they had gotten an inferior performance to the one of not benefited in relation to the analyzed agricultural pointers. One concluded that the influence of the PRONAF in the performance of the agricultural pointers of the subsistence farmings is very small
Cyclin D1 silencing suppresses tumorigenicity, impairs DNA double strand break repair and thus radiosensitizes androgenindependent prostate cancer cells to DNA damage.
Patients with hormone-resistant prostate cancer (PCa) have higher biochemical failure rates following radiation therapy (RT). Cyclin D1 deregulated expression in PCa is associated with a more aggressive disease: however its role in radioresistance has not been determined. Cyclin D1 levels in the androgen-independent PC3 and 22Rv1 PCa cells were stably inhibited by infecting with cyclin D1-shRNA. Tumorigenicity and radiosensitivity were investigated using in vitro and in vivo experimental assays. Cyclin D1 silencing interfered with PCa oncogenic phenotype by inducing growth arrest in the G1 phase of cell cycle and reducing soft agar colony formation, migration, invasion in vitro and tumor formation and neo-angiogenesis in vivo. Depletion of cyclin D1 significantly radiosensitizes PCa cells by increasing the RT-induced DNA damages by affecting the NHEJ and HR pathways responsible of the DNA double-strand break repair. Following treatment of cells with RT the abundance of a biomarker of DNA damage, γ-H2AX, was dramatically increased in sh-cyclin D1 treated cells compared to shRNA control. Concordant with these observations DNA-PKcs-activation and RAD51-accumulation, part of the DNA double-strand break repair machinery, were reduced in shRNA-cyclin D1 treated cells compared to shRNA control. We further demonstrate the physical interaction between CCND1 with activated-ATM, -DNA-PKcs and RAD51 is enhanced by RT. Finally, siRNA-mediated silencing experiments indicated DNA-PKcs and RAD51 are downstream targets of CCND1-mediated PCa cells radioresistance. In summary, these observations suggest that CCND1 is a key mediator of PCa radioresistance and could represent a potential target for radioresistent hormone-resistant PCa
Vaccination with Ad5 Vectors Expands Ad5-Specific CD8+ T Cells without Altering Memory Phenotype or Functionality
Adenoviral (Ad) vaccine vectors represent both a vehicle to present a novel antigen to the immune system as well as restimulation of immune responses against the Ad vector itself. To what degree Ad-specific CD8(+) T cells are restimulated by Ad vector vaccination is unclear, although such knowledge would be important as vector-specific CD8(+) T cell expansion could potentially further limit Ad vaccine efficacy beyond Ad-specific neutralizing antibody alone.Here we addressed this issue by measuring human Adenovirus serotype 5 (Ad5)-specific CD8(+) T cells in recipients of the Merck Ad5 HIV-1 vaccine vector before, during, and after vaccination by multicolor flow cytometry. Ad5-specific CD8(+) T-cells were detectable in 95% of subjects prior to vaccination, and displayed primarily an effector-type functional profile and phenotype. Peripheral blood Ad5-specific CD8(+) T-cell numbers expanded after Ad5-HIV vaccination in all subjects, but differential expansion kinetics were noted in some baseline Ad5-neutralizing antibody (Ad5 nAb) seronegative subjects compared to baseline Ad5 nAb seropositive subjects. However, in neither group did vaccination alter polyfunctionality, mucosal targeting marker expression, or memory phenotype of Ad5-specific CD8(+) T-cells.These data indicate that repeat Ad5-vector administration in humans expands Ad5-specific CD8(+) T-cells without overtly affecting their functional capacity or phenotypic properties. This is a secondary analysis of samples collected during the 016 trial. Results of the Merck 016 trial safety and immunogenicity have been previously published in the journal of clinical infectious diseases [1].ClinicalTrials.gov NCT00849680[http://www.clinicaltrials.gov/show/NCT00849680]
A High Statistics Measurement of the Lambdac+ Lifetime
A high statistics measurement of the Lambdac+ lifetime from the Fermilab
fixed-target FOCUS photoproduction experiment is presented. We describe the
analysis technique with particular attention to the determination of the
systematic uncertainty. The measured value of 204.6 +/- 3.4 (stat.) +/- 2.5
(syst.) fs from 8034 +/- 122 Lambdac -> pKpi decays represents a significant
improvement over the present world average.Comment: Submitted to Physical Review Letter
A Measurement of the Ds+ Lifetime
A high statistics measurement of the Ds+ lifetime from the Fermilab
fixed-target FOCUS photoproduction experiment is presented. We describe the
analysis of the two decay modes, Ds+ -> phi(1020)pi+ and Ds+ ->
\bar{K}*(892)0K+, used for the measurement. The measured lifetime is 507.4 +/-
5.5 (stat.) +/- 5.1 (syst.) fs using 8961 +/- 105 Ds+ -> phi(1020)pi+ and 4680
+/- 90 Ds+ -> \bar{K}*(892)0K+ decays. This is a significant improvement over
the present world average.Comment: 5 pages, 3 figures, 2 tables, submitted to PR
Search for CP Violation in the decays D+ -> K_S pi+ and D+ -> K_S K+
A high statistics sample of photo-produced charm from the FOCUS(E831)
experiment at Fermilab has been used to search for direct CP violation in the
decays D+->K_S pi+ and D+ -> K_S K+. We have measured the following asymmetry
parameters relative to D+->K-pi+pi+: A_CP(K_S pi+) = (-1.6 +/- 1.5 +/- 0.9)%,
A_CP(K_S K+) = (+6.9 +/- 6.0 +/- 1.5)% and A_CP(K_S K+) = (+7.1 +/- 6.1 +/-
1.2)% relative to D+->K_S pi+. The first errors quoted are statistical and the
second are systematic. We also measure the relative branching ratios:
\Gamma(D+->\bar{K0}pi+)/\Gamma(D+->K-pi+pi+) = (30.60 +/- 0.46 +/- 0.32)%,
\Gamma(D+->\bar{K0}K+)/\Gamma(D+->K-pi+pi+) = (6.04 +/- 0.35 +/- 0.30)% and
\Gamma(D+->\bar{K0}K+)/\Gamma(D+->\bar{K0}pi+) = (19.96 +/- 1.19 +/- 0.96)%.Comment: 4 pages, 3 figure
A measurement of branching ratios of and hadronic decays to four-body final states containing a
We have studied hadronic four-body decays of and mesons with a
in the final state using data recorded during the 1996-1997 fixed-target
run at Fermilab high energy photoproduction experiment FOCUS. We report a new
branching ratio measurement of . We make the first observation
of three new decay modes with branching ratios ,
\Gamma(D^+\to\K_S K^+ K^-\pi^+)/\Gamma(D^+\to K_S
\pi^+\pi^+\pi^-)=0.0077\pm0.0015\pm0.0009, and , where
in each case the first error is statistical and the second error is systematic.Comment: 4 pages, 1 table, 2 figures, submitted to Physical Review Letter
New FOCUS results on charm mixing and CP violation
We present a summary of recent results on CP violation and mixing in the
charm quark sector based on a high statistics sample collected by
photoproduction experiment FOCUS (E831 at Fermilab). We have measured the
difference in lifetimes for the decays: and . This translates into a measurement of the mixing parameter in
the \d0d0 system, under the assumptions that is an equal mixture of
CP odd and CP even eigenstates, and CP violation is negligible in the neutral
charm meson system. We verified the latter assumption by searching for a CP
violating asymmetry in the Cabibbo suppressed decay modes , and . We show preliminary
results on a measurement of the branching ratio .Comment: 9 pages, 6 figures, requires espcrc2.sty. Presented by S.Bianco at
CPConf2000, September 2000, Ferrara (Italy). In this revision, fixed several
stylistic flaws, add two significant references, fixed a typo in Tab.
- …