Restoring Relationship: How the Methodologies of Wangari Maathai and the Green Belt Movement in Post-Colonial Kenya Achieve Environmental Healing and Women\u27s Empowerment

The effects of the colonial project in Kenya created multi-faceted damages to the land and indigenous people-groups. Using the lens of ecofeminism, this study examines the undergirding structures that produce systems such as colonization that oppress and destroy land, people, and other beings. By highlighting the experience of the Kikuyu people within the Kenyan colonial program, the innovative and ingenious response of Wangari Maathai\u27s Green Belt Movement proves to be a relevant and effective counter to women\u27s disempowerment and environmental devastation in a post-colonial nation. The approach of the Green Belt Movement offers a unique and accessible method for empowering women, restoring the land, and addressing loss of cultural identity, while also contributing a theoretical template for addressing climate change

Characterization of a Novel Clade of Transporters in Phytophthora

The oomycete Phytophthora parasitica has a worldwide distribution and is an economically important pathogen of more than 100 species4. RNA-seq analysis showed that one gene, PPTG_16698 has the 5th highest level of expression of all transport proteins in the zoospore stage, and is highly conserved throughout Phytophthora species. This project attempts to characterize the important biological role that PPTG_16698 plays in P. parasitica and other oomycetes. Three strategies have been implemented to accomplish this goal: growth analysis by heterologous expression in yeast, metabolite analysis in yeast, and construction of a GFP fusion protein to enable localization of the gene in oomycete hyphae by confocal microscopy. Confocal microscopy is expected to confirm the vacuolar localization of this gene. If this gene is localized to a vacuolar membrane, then heterologous expression in yeast should result in differential accumulation of metabolites mobilized by this transporter. In preliminary growth assays, expression of this gene did not inhibit the growth of yeast. Therefore, expression of the gene does not result in sequestering of a growth-limiting metabolite. To determine whether expression of the transporter results in the accumulation of polyamines, which are organic compounds necessary for growth in eukaryotes, polyamine levels will be measured by dansylation of amines and separation by HPLC. Other metabolites will be assayed by liquid chromatography mass spectroscopy analysis

Comparison of A-mode and B-mode Ultrasound for Measurement of Subcutaneous Fat

With lower-cost devices and technologic advancements, ultrasound has been undergoing a resurgence as a method to measure subcutaneous adipose tissue. We aimed to determine whether a low-cost, 2.5-MHz amplitude (A-mode) ultrasound, designed specifically for body composition assessment, could produce subcutaneous fat thickness measurements comparable to an expensive, 12-MHz brightness (B-mode) device. Fat thickness was measured on 40 participants (20 female, 20 male; 29.7 ± 11.1 y of age; body mass index 24.9 ± 4.5 kg/m2) at 7 sites (chest, subscapula, mid-axilla, triceps, abdomen, suprailiac and thigh) with both devices. Intraclass correlations exceeded 0.75 at all measurement sites. Mean differences in fat thickness were not significantly different (p \u3e 0.05) and within ± 1.0 mm. Variability between devices was greatest at the abdomen, the site with the greatest thickness. The low-cost, low-resolution A-mode ultrasound provides subcutaneous fat thickness measurements similar to the more expensive, high-resolution B-mode ultrasound

Nanoscale rheology: dynamic mechanical analysis over a broad and continuous frequency range using photothermal actuation atomic force microscopy

Polymeric materials are widely used in industries ranging from automotive to biomedical. Their mechanical properties play a crucial role in their application and function and arise from the nanoscale structures and interactions of their constitutive polymer molecules. Polymeric materials behave viscoelastically, i.e., their mechanical responses depend on the time scale of the measurements; quantifying these time-dependent rheological properties at the nanoscale is relevant to develop, for example, accurate models and simulations of those materials, which are needed for advanced industrial applications. In this paper, an atomic force microscopy (AFM) method based on the photothermal actuation of an AFM cantilever is developed to quantify the nanoscale loss tangent, storage modulus, and loss modulus of polymeric materials. The method is then validated on styrene–butadiene rubber (SBR), demonstrating the method’s ability to quantify nanoscale viscoelasticity over a continuous frequency range up to 5 orders of magnitude (0.2–20,200 Hz). Furthermore, this method is combined with AFM viscoelastic mapping obtained with amplitude modulation–frequency modulation (AM–FM) AFM, enabling the extension of viscoelastic quantification over an even broader frequency range and demonstrating that the novel technique synergizes with preexisting AFM techniques for quantitative measurement of viscoelastic properties. The method presented here introduces a way to characterize the viscoelasticity of polymeric materials and soft and biological matter in general at the nanoscale for any application

T Helper 1 and T Helper 2 Cells Are Pathogenic in an Antigen-specific Model of Colitis

Dysregulated T cell responses to enteric bacteria have been implicated as a common mechanism underlying pathogenesis in rodent models of colitis. However, the bacterial species and T cell specificities that induce disease have been poorly defined. We have developed a model system in which target antigen, bacterial host, and corresponding T cell specificity are defined. OVA-specific T cells from DO11.RAG-2−/− TCR transgenic mice were transferred into RAG-2−/− recipients whose intestinal tracts were colonized with OVA-expressing or control Escherichia coli. Transfer of antigen-naive DO11.RAG-2−/− T cells into recipients colonized with OVA-E. coli resulted in enhanced intestinal recruitment and cell cycling of OVA-specific T cells; however, there was no development of disease. In contrast, transfer of polarized T helper (Th) 1 and Th2 populations resulted in severe wasting and colitis in recipients colonized with OVA-expressing but not control E. coli. The histopathologic features of disease induced by Th1 and Th2 transfers were distinct, but disease severity was comparable. Induction of disease by both Th1 and Th2 transfers was dependent on bacterially associated OVA. These results establish that a single bacterially associated antigen can drive the progression of colitis mediated by both Th1 and Th2 cells and provide a new model for understanding the immunoregulatory interactions between T cells responsive to gut floral antigens

Comparison of Perioperative Outcomes Between Holmium Laser Enucleation of the Prostate and Robot-Assisted Simple Prostatectomy

Objectives: To compare perioperative outcomes for patients undergoing holmium laser enucleation of the prostate (HoLEP) and robotic-assisted simple prostatectomy (RSP) for benign prostatic hypertrophy (BPH).Methods: Patient demographics and perioperative outcomes were compared between 600 patients undergoing HoLEP and 32 patients undergoing RSP at two separate academic institutions between 2008 and 2015.Results: Patients undergoing HoLEP and RSP had comparable ages (71 vs 71, p = 0.96) and baseline American Urological Association Symptom Scores (20 vs 24, p = 0.21). There was no difference in mean specimen weight (96 g vs 110 g, p = 0.15). Mean operative time was reduced in the HoLEP cohort (103 minutes vs 274 minutes, p < 0.001). Patients undergoing HoLEP had lesser decreases in hemoglobin, decreased transfusions rates, shorter hospital stays, and decreased mean duration of catheterization. There was no difference in the rate of complications Clavien grade 3 or greater (p = 0.33).Conclusions: HoLEP and RSP are both efficacious treatments for large gland BPH. In expert hands, HoLEP appears to have a favorable perioperative profile. Further studies are necessary to compare long-term efficacy, cost, and learning curve influences, especially as minimally invasive approaches become more widespread

Composition of the Survival Motor Neuron (SMN) complex in Drosophila melanogaster

Spinal Muscular Atrophy (SMA) is caused by homozygous mutations in the human survival motor neuron 1 (SMN1) gene. SMN protein has a well-characterized role in the biogenesis of small nuclear ribonucleoproteins (snRNPs), core components of the spliceosome. SMN is part of an oligomeric complex with core binding partners, collectively called Gemins. Biochemical and cell biological studies demonstrate that certain Gemins are required for proper snRNP assembly and transport. However, the precise functions of most Gemins are unknown. To gain a deeper understanding of the SMN complex in the context of metazoan evolution, we investigated its composition in Drosophila melanogaster. Using transgenic flies that exclusively express Flag-tagged SMN from its native promoter, we previously found that Gemin2, Gemin3, Gemin5, and all nine classical Sm proteins, including Lsm10 and Lsm11, co-purify with SMN. Here, we show that CG2941 is also highly enriched in the pulldown. Reciprocal co-immunoprecipitation reveals that epitope-tagged CG2941 interacts with endogenous SMN in Schneider2 cells. Bioinformatic comparisons show that CG2941 shares sequence and structural similarity with metazoan Gemin4. Additional analysis shows that three other genes (CG14164, CG31950 and CG2371) are not orthologous to Gemins 6-7-8, respectively, as previously suggested. In D.melanogaster, CG2941 is located within an evolutionarily recent genomic triplication with two other nearly identical paralogous genes (CG32783 and CG32786). RNAi-mediated knockdown of CG2941 and its two close paralogs reveals that Gemin4 is essential for organismal viability

The Grizzly, April 27, 2006

Full Auditorium Discusses Solutions to Diversity Issues at Forum • A Senior Reflects on Her Ursinus Experience • Annual Student Art Show Opens • Crowding First Base • One Last Look Back • Students\u27 Artwork Featured Permanently in Myrin Library • Exploring the Nature of Suburban Sprawl • Opinions: Unnecessary Police Watch; Organic Obsession • Year in Review, Dynasties and Disappointmentshttps://digitalcommons.ursinus.edu/grizzlynews/1714/thumbnail.jp

Composition of the Survival Motor Neuron (SMN) Complex in \u3cem\u3eDrosophila melanogaster\u3c/em\u3e

Spinal Muscular Atrophy (SMA) is caused by homozygous mutations in the human survival motor neuron 1 (SMN1) gene. SMN protein has a well-characterized role in the biogenesis of small nuclear ribonucleoproteins (snRNPs), core components of the spliceosome. SMN is part of an oligomeric complex with core binding partners, collectively called Gemins. Biochemical and cell biological studies demonstrate that certain Gemins are required for proper snRNP assembly and transport. However, the precise functions of most Gemins are unknown. To gain a deeper understanding of the SMN complex in the context of metazoan evolution, we investigated its composition in Drosophila melanogaster. Using transgenic flies that exclusively express Flag-tagged SMN from its native promoter, we previously found that Gemin2, Gemin3, Gemin5, and all nine classical Sm proteins, including Lsm10 and Lsm11, co-purify with SMN. Here, we show that CG2941 is also highly enriched in the pulldown. Reciprocal co-immunoprecipitation reveals that epitope-tagged CG2941 interacts with endogenous SMN in Schneider2 cells. Bioinformatic comparisons show that CG2941 shares sequence and structural similarity with metazoan Gemin4. Additional analysis shows that three other genes (CG14164, CG31950 and CG2371) are not orthologous to Gemins 6-7-8, respectively, as previously suggested. In D.melanogaster, CG2941 is located within an evolutionarily recent genomic triplication with two other nearly identical paralogous genes (CG32783 and CG32786). RNAi-mediated knockdown of CG2941 and its two close paralogs reveals that Gemin4 is essential for organismal viability