Trispecific antibody targeting HIV-1 and T cells activates and eliminates latently-infected cells in HIV/SHIV infections.

Agents that can simultaneously activate latent HIV, increase immune activation and enhance the killing of latently-infected cells represent promising approaches for HIV cure. Here, we develop and evaluate a trispecific antibody (Ab), N6/αCD3-αCD28, that targets three independent proteins: (1) the HIV envelope via the broadly reactive CD4-binding site Ab, N6; (2) the T cell antigen CD3; and (3) the co-stimulatory molecule CD28. We find that the trispecific significantly increases antigen-specific T-cell activation and cytokine release in both CD4 <sup>+</sup> and CD8 <sup>+</sup> T cells. Co-culturing CD4 <sup>+</sup> with autologous CD8 <sup>+</sup> T cells from ART-suppressed HIV <sup>+</sup> donors with N6/αCD3-αCD28, results in activation of latently-infected cells and their elimination by activated CD8 <sup>+</sup> T cells. This trispecific antibody mediates CD4 <sup>+</sup> and CD8 <sup>+</sup> T-cell activation in non-human primates and is well tolerated in vivo. This HIV-directed antibody therefore merits further development as a potential intervention for the eradication of latent HIV infection

Cost of Virtual Machine Live Migration in Clouds: A Performance Evaluation

Virtualization has become commonplace in modern data centers, often referred as "computing clouds". The capability of virtual machine live migration brings benefits such as improved performance, manageability and fault tolerance, while allowing workload movement with a short service downtime. However, service levels of applications are likely to be negatively affected during a live migration. For this reason, a better understanding of its effects on system performance is desirable. In this paper, we evaluate the effects of live migration of virtual machines on the performance of applications running inside Xen VMs. Results show that, in most cases, migration overhead is acceptable but cannot be disregarded, especially in systems where availability and responsiveness are governed by strict Service Level Agreements. Despite that, there is a high potential for live migration applicability in data centers serving modernInternet applications. Our results are based on a workload covering the domain of multi-tier Web 2.0 applications.Comment: CloudCom 200