Implementación del método de vencimiento de normas en la pistola 50 m con aumento progresivo de las distancias a través de tareas específicas a fin de que los atletas ganen en confianza durante su desempeño

El presente trabajo trata sobre la implementación práctica del método vencimiento de normas en la pistola 50m con aumento progresivo de la distancia en atletas de la ESPA “Ormani Arenado Llonch” de la provincia de Pinar del Río, debido a la poca estabilidad de los resultados en este evento y lo difícil que se ha convertido la norma de los 500 puntos para estos atletas a través de los años anteriores. Haciendo una breve búsqueda de información sobre los entrenamientos llevados a cabo por los entrenadores anteriores y la falta de confianza manifestada por los atletas, fue así que se decidió hacer un poco mas dinámico y entretenido el entrenamiento; también nos percatamos de que los atletas tenían buena preparación técnica con algunos pequeños errores pero les parecía muy difícil la norma de los 500 puntos y mucho menos pensar en algo más; se llevó a cabo una planificación de objetivos semanales y comenzando por la distancia de 20 metros e ir aumentando 5 metros hasta llegar a los 50 metros, un objetivo especifico a vencer para cada distancia, para así, con la sistematicidad que caracteriza al proceso, relacionando las cargas de preparación especial y técnico-táctica con las distancias para lograr demostrar que se pudo alcanzar una agrupación de 8 de 10 disparos en cada serie para finalmente obtener una puntuación de 500 o más puntos en cada atleta en las Olimpiadas Juveniles del 2009. Para ello se trazaron diferentes estrategias que ayudaron al dinamismo del entrenamiento y el trabajo colectivo, todas ellas sustentadas bajo una serie de procedimientos metodológicos científicamente demostrados y con poca divulgación para su utilizació

Cellular and humoral functional responses after BNT162b2 mRNA vaccination differ longitudinally between naive and subjects recovered from COVID-19

We have analyzed BNT162b2 vaccine-induced immune responses in naive subjects and individuals recovered from coronavirus disease 2019 (COVID-19), both soon after (14 days) and later after (almost 8 months) vaccination. Plasma spike (S)-specific immunoglobulins peak after one vaccine shot in individuals recovered from COVID-19, while a second dose is needed in naive subjects, although the latter group shows reduced levels all along the analyzed period. Despite how the neutralization capacity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mirrors this behavior early after vaccination, both groups show comparable neutralizing antibodies and S-specific B cell levels late post-vaccination. When studying cellular responses, naive individuals exhibit higher SARS-CoV-2-specific cytokine production, CD4+ T cell activation, and proliferation than do individuals recovered from COVID-19, with patent inverse correlations between humoral and cellular variables early post-vaccination. However, almost 8 months post-vaccination, SARS-CoV-2-specific responses are comparable between both groups. Our data indicate that a previous history of COVID-19 differentially determines the functional T and B cell-mediated responses to BNT162b2 vaccination over time.C.d.F., J.G.-P., and J.A. are supported by Instituto de Salud Carlos III (ISCII). We thank JM Ligos and Cytek Biosciences for their technical support. Research in E.L.-C.’s lab was supported by Fundación Familia Alonso, Santander Bank, Real Seguros, Fundación Mutua Madrileña, Fundación Uria, Fundación La Caixa, and Ayuntamiento de Madrid.S

REPORTE CORTO/SHORT REPORT - A RECOMBINANT TMPA FUSION PROTEIN. EFFECT OF IL-2 AND HISTIDIN DOMAINS ON EXPRESSION

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Tercera edad y valores

El envejecimiento poblacional, resultante de los avances en el campo de las ciencias fundamentalmente, deviene tema de gran interés en la actualidad. Entre los grandes retos asumidos por gobiernos y organismos internacionales está lograr una mayor calidad de vida en las personas que rebasan los 60 años, más cuando la esperanza de vida al nacer sigue incrementándose. No se ha superado aún la tendencia a considerar al adulto mayor como una carga, para la familia y la sociedad, por lo que con este trabajo se trata de mejorar la influencia sobre este sector poblacional dándole al reforzamiento de valores una connotación primordial en aras de que cada individuo sea protagonista en el logro de su propio bienestar físico y mental

Tercera edad y valores

El envejecimiento poblacional, resultante de los avances en el campo de las ciencias fundamentalmente, deviene tema de gran interés en la actualidad. Entre los grandes retos asumidos por gobiernos y organismos internacionales está lograr una mayor calidad de vida en las personas que rebasan los 60 años, más cuando la esperanza de vida al nacer sigue incrementándose. No se ha superado aún la tendencia a considerar al adulto mayor como una carga, para la familia y la sociedad, por lo que con este trabajo se trata de mejorar la influencia sobre este sector poblacional dándole al reforzamiento de valores una connotación primordial en aras de que cada individuo sea protagonista en el logro de su propio bienestar físico y mental

Selection of phage displayed antibodies based on kinetic constants

The display of antibody fragments on the surface of filamentous bacteriophages and the selection of binders from antibody libraries have provided powerful tools to generate human antibodies. We reported recently a new concept (SAP system) for the selection of specific phages by linking antigenic recognition and phage replication, using a soluble fusion protein containing the antigen and a fragment of the M13 coat protein 3. In this investigation, a model library has been composed using six different antibody fragments which were characterized individually regarding their k(ass), k(diss) and K(a). All Feb fragments were specific for a 15 amino acid region of the V3 loop of gp120 (HIV-1). We demonstrated that the SAP system could discriminate between the kinetic parameters of each clone, using different selection strategies. Phages expressing high affinity clones were selected preferentially using low doses of antigen but clones of lower affinity also could be selected by increasing the antigen concentration or using a preselection procedure. Phages expressing antibody fragment with high association or low dissociation rate constants were retrieved by utilizing short contact times between antigen and antibody or antigen-chase conditions

In vitro immunization of naive human B cells yields high affinity immunoglobulin G antibodies as illustrated by phage display.

In vitro antibody responses to a synthetic immunogen, consisting of both a B cell [V3 loop of gp120 from human immunodeficiency virus type 1 (HIV-1)] and a T-helper epitope (15 amino acids of tetanus toxoid) was studied. The in vitro activation was performed by primary and secondary in vitro immunizations, using lymphocytes obtained from uninfected, seronegative donors. Analysis of the in vitro immune response demonstrated an antigen-specific isotype switch, which was dependent on the presence of antigen-specific T-helper cells, CD40 ligation and antigen. Antibody libraries were constructed from cells derived directly from the naive donors, or from primary or secondary in vitro immunized B cells. Five libraries were displayed on filamentous phage and selected for anti-V3-specific Fab fragments, using a selection approach that linked recognition and phage replication. A panel of 19 recombinant antigen-specific Fab. representing different phases of the humoral in vitro immune response were sequenced, expressed and analysed using a biosensor. Recombinant Fab fragments derived from cultures on day 12 exhibited an increase in affinity of close to two orders of magnitude compared to those obtained from cells primary immunized for 7 days. This study provides the first evidence that an antigen-specific in vitro immune response can yield high-affinity immunoglobulinG antibodies

A bacterial single-chain Fv antibody fragment that inhibits binding of its parental anti-E-selectin monoclonal antibody to activated human endothelial cells.

Division of Immunotechnology and Diagnostics, Center for Genetic Engineering and Biotechnology, La Habana, Cuba.Using the polymerase chain reaction, we cloned, modified, and linked antibody variable (V) region coding genes from a mouse hybridoma, and produced a bacterial single-chain Fv (scFv) antibody fragment specific for E-Selectin. A vector of pBR322 origin, bearing the tryptophan promoter and the ompA bacterial signal peptide, was used to direct scFv expression to periplasm. The vector included a six-histidine coding sequence 5' to the scFv for the purification of the expressed protein using immobilized metal affinity chromatography (IMAC). We found that the VH-Linker-VL 32-33 kDa scFv remained insoluble after cellular fractionation, and transmission electron microscopy showed the new protein to be present in the periplasm as inclusion bodies. The scFv was solubilized using urea, purified using IMAC, and renatured to its active form. In a competitive enzyme-linked immunosorbent assay with activated human vein endothelial cells in the solid phase, the scFv competed for binding with the original monoclonal antibody

Identification of sSIGLEC5 and sLAG3 as New Relapse Predictors in Lung Cancer

Lung cancer (LC) continues to be the leading cause of cancer-related deaths in both men and women worldwide. After complete tumour resection, around half of the patients suffer from disease relapse, emphasising the critical need for robust relapse predictors in this disease. In search of such biomarkers, 83 patients with non-microcytic lung cancer and 67 healthy volunteers were studied. Pre-operative levels of sSIGLEC5 along with other soluble immune-checkpoints were measured and correlated with their clinical outcome. Soluble SIGLEC5 (sSIGLEC5) levels were higher in plasma from patients with LC compared with healthy volunteers. Looking into those patients who suffered relapse, sSIGLEC5 and sLAG3 were found to be strong relapse predictors. Following a binary logistic regression model, a sSIGLEC5 + sLAG3 score was established for disease relapse prediction (area under the curve 0.8803, 95% confidence intervals 0.7955–0.9652, cut-off > 2.782) in these patients. Based on score cut-off, a Kaplan–Meier analysis showed that patients with high sSIGLEC5 + sLAG3 score had significantly shorter relapse-free survival (p ≤ 0.0001) than those with low sSIGLEC5 + sLAG3 score.Our study suggests that pre-operative sSIGLEC5 + sLAG3 score is a robust relapse predictor in LC patients