Toward a Biocommunicable Cartography of Health Decision-Making in the Amazon Basin of Ecuador

This paper comprises a critical, ethnographic study of health communication in a rural community of Amazonian Ecuador. By synthesizing approaches from anthropology, discourse studies, and public health, the study explores how conversations influence health decisions, how communities understand health systems, and how macrostructural discourse changes the political economy of healthcare in Ecuador. My work draws on the recent theoretical development of ‘biocommunicability’ in anthropology as well as earlier sociological research on knowledge construction. Most importantly, this paper offers a critique of current interventions by NGOs in the region

Boy Scouts March

https://digitalcommons.library.umaine.edu/mmb-ps/1777/thumbnail.jp

ANALYSIS OF PHARMACEUTICALS AND BIOMOLECULES USING HPLC COUPLED TO ICP-MS AND ESI-MS

The work described within this thesis explores the use of HPLC coupled with ICPMS and ESI-MS in order to develop novel methods which overcome specific analytical challenges in the pharmaceutical industry. A membrane desolvation interface has been evaluated for coupling high performance liquid chromatography (HPLC) with inductively coupled plasma mass spectrometry (ICP-MS). Desolvation of the sample prior to reaching the plasma was shown to facilitate a versatile coupling of the two instrumental techniques, enabling chromatographic eluents containing up to 100 % organic to be used. This interface also allowed gradient elution to be used with ICP-MS. Tris(2,4,6-trimethoxyphenyl)phosphonium propylamine bromide (TMPP) was used for the derivatisation of maleic, fumaric, sorbic and salicylic acids to facilitate determination by HPLC-electrospray ionisation tandem mass spectrometry (ESIMS/ MS) in positive ion mode. Improvements in detection limits post-derivatisation were achieved, and this method was successfully used for the determination of sorbic acid in a sample of Panadol™. HPLC coupled with sector field inductively coupled plasma mass spectrometry (SF-ICP-MS) has been used for the determination of maleic, sorbic and fumaric acids after derivatisation with TMPP. This allowed 31P+ selective detection to be performed for these compounds, which are normally undetectable by ICP-MS. Optimal reagent conditions for the derivatisation of 0.1 mM maleic acid were: 1 mM TMPP; 10 mM 2-chloro-1-methylpyridinium iodide (CMPI); 11 mM triethylamine. The efficiency of the derivatisation reaction was estimated to be between 10-20%. Detection limits, estimated as 3 times baseline noise, were 0.046 nmol for TMPP and 0.25 nmol for derivatised maleic acid, for a 5 f.JL injection. Following on from this, a novel derivatising reagent, tris(3,5-dibromo-2,4,6- trimethoxyphenyl) phosphonium propylamine bromide (BrTMPP), was synthesised and subsequently characterised by proton NMR spectroscopy and ESI-MS. This was utilised to derivatise maleic acid, with a 9-fold increase in sensitivity gained when analysed by bromine selective detection as apposed to phosphorus selective ICP-MS. This derivatising reagent (BrTMPP) was also utilised to determine the degree of phosphorylation on phosphorylated peptides. A phosphorus containing carboxylic acid was successfully derivatised and the correct Br:P ratio was determined for this compound by ICP-MS. However, phosphorylated peptides were not successfully derivatised by BrTMPP. A combination of UV and phosphorus selective ICP-MS was also used to distinguish between phosphorylated and un-phosphorylated peptides after HPLC separation.GlaxoSmithKiin

External States and the Indian Ocean: The Potential for Confrontation

A study of the common interests of internal and external states in the Indian Ocean and identification of the methods, and their potential for confrontation, employed by external powers in pursuit of their interests. The historical interests of external powers in the Indian Ocean are depicted. Common interests, which have commanded the attention of internal states and external powers during recent years, are identified. The Indian Ocean is divided into five regions, each region characterized by the aforementioned common interests. The assets, mutual relations and interests of the littoral states comprising each region are described. The interests and methods of projecting these interests by China, Japan, Western Europe, Soviet Union and the United States are delineated. The paper finds that external power interests are competing at an increasing rate in the Indian Ocean area. As the interest converge, the potential for confrontation, particularly between the United States and Russia, resulting from methods of operation and uncontrollable events, increases. U.S. policy makers must clearly define U.S.-Indian Ocean objectives, to maximize effective utilization of resources in pursuit thereof

U.S. Law of the Sea Cruise to Map the Foot of the Slope and 2500-m Isobath of the Northeast U.S. Atlantic Continental Margin: Legs 4 and 5. Cruise Report

U.S. Law of the Sea cruise to map the foot of the slope and 2500-m isobath of the Northeast US Atlantic continental margin: Legs 4 and 5 CRUISES PF05-1 and 2 April 25 to June 30, 2005 Norfolk, VA to Port Canaveral, F

Towards a Java Subtyping Operad

The subtyping relation in Java exhibits self-similarity. The self-similarity in Java subtyping is interesting and intricate due to the existence of wildcard types and, accordingly, the existence of three subtyping rules for generic types: covariant subtyping, contravariant subtyping and invariant subtyping. Supporting bounded type variables also adds to the complexity of the subtyping relation in Java and in other generic nominally-typed OO languages such as C# and Scala. In this paper we explore defining an operad to model the construction of the subtyping relation in Java and in similar generic nominally-typed OO programming languages. Operads, from category theory, are frequently used to model self-similar phenomena. The Java subtyping operad, we hope, will shed more light on understanding the type systems of generic nominally-typed OO languages.Comment: 13 page

Functional Studies of Genetic Variants in TRPM7 and AKAP9 – Two Candidate Genes for Stillbirth

For every 200 births in the UK, one will end in a stillbirth. Stillbirth is classified as a baby born dead after 24 weeks gestation. Mutations in genes that cause ion channelopathies are known to cause sudden cardiac death in adults and children. Prenatal diagnosis of LQT has been possible for decades, creating a disease spectrum where channelopathies may fatally influence pregnancy. We sequenced 35 candidate genes in 70 unexplained stillbirth cases. Thirty-nine cases harboured a predicted damaging protein missense variant. Two novel and two rare variants were observed in the transient receptor potential melastatin 7 (TRPM7) gene and five rare genetic variants were found in A-kinase anchor protein 9 (AKAP9). The aim of this PhD was to perform functional studies of these variants in TRPM7 and AKAP9. TRPM7 is an ion channel indispensable for mouse cardiogenesis. Two TRPM7 variants (p.G179V and p.T860M) showed significantly reduced current compared to wild-type channels. Conversely, cells expressing p.R494Q TRPM7, had a significant increase in current compared to WT channels, but only in CHO-K1 cells. Western blot analyses failed to detect full length TRPM7 in cells transfected with either p.G179V or p.T860M compared to wild-type expressing cells. Proteosomal inhibition using MG132 produced a small but visible band in p.T860M transfected cells. Expression of TRPM7 in iPSC-derived cardiomyocytes increases during cell maturation, and TRPM7-like current was measured in 20-23 day old cardiomyocytes. AKAP9 is required to couple adrenergic stimulation in the heart with faster cardiac repolarisation. Cells expressing WT AKAP9 alongside the KCNQ1/KCNE1 potassium channel responded to β-adrenergic stimulation, however those transfected with p.A3043T AKAP9 did not respond to treatment with forskolin. Our analyses supports two deleterious variants in TRPM7 and one in AKAP9 in unexplained stillbirth cases. These heterozygous variants could lead to haploinsufficiency and may be a cause of stillbirth

Latent murderousness: an exploration of the nature and quality of object relations in rage-type murderers

In this dissertation I investigate the intrapsychic make-up of rage-type offenders and explore the psychodynamics of the act of murder itself The dissertation begins with a discussion on the defining features of the act of rage-type murder. I then consider the role of personality characteristics and psychopathology in individuals who have committed such offences. With the basic features of the offender and act itself outlined, the following section reviews key areas of debate regarding the psychodynamics of violence and the intrapsychic make-up of the rage-type murderer. I first explore the nature of aggression as debated in psychoanalysis and conclude that the views expressed are often unn,ecessarily polarized regarding the origins of aggression and suggest that the specifics of particular types of aggression require consideration in order to assess their intrapsychic nature. The specifics of rage and violence are discussed with this in mind. In the second chapter of this section I develop a number of intrapsychic dimensions to be used in understanding how different types of violence are constituted. Psychodynamic contributions towards understanding rage-type murder, as a specific form of violence, are then discussed. Following this review, a number of directive ~uestions are formulated regarding (1) the intrapsychic dimension of rage-type murder; (2) the pJ;esence of the borderline personality in such offender~ and its intrapsychic nature; and (3) the_ specific psychodynamics that lie behind what is argued to be a defensive act of murder. A multiple case study approach, using nine imprisoned rage-type offenders, is used to further explore the above issues. Court summary reports, the Thematic Apperception Test and the Psychoanalytic Research Interview comprised the research material, with particular emphasis placed on the interview material. The interview is approached from a psychoanalytic perspective and I develop some theoretical, technical and analytical guidelines to try to broaden Jhe use of psychoanalysis in the research domain. Findings of the research reveal a specific kind of defensive organization that is characterized by a constellation of object relations that I term the 'narcissistic exoskeleton'. I suggest that these findings best fit the description of a particular kind of borderline personality organization typified by apparent 'normality'. Other prominent aspects of the dimensions of violence observed in these cases include: (1) a poor representational capacity; (2) an interactional style characterized by uncontainable projective exchanges between victim and offender; (3) a collusive primary object relationship combined with the absence of an internalized 'third object'; (4) a 'two-faced' superego structure; (5) the internalization of traumatic experience that has become associated with a bad object system; (6) phantasies of restoring ideal good in external objects alongside conscious fantasies of annihilation. Within the context of these factors the intrapsychic events that lead to the act itself are discussed. It is found that a collapse of the 'narcissistic exoskeleton', the intrusion of the bad object system and the unbearable shame that this evokes in the offender are prominent features of what culminates in an act of explosive rage and projective identification. Some of the implications of my research are briefly discussed in the concluding chapter

Preliminary analysis of amplitude and phase fluctuations in the JAPE multiple tone data to distances of 500 meters

The JAPE short range data provide a good opportunity for studying phase and amplitude fluctuations of acoustic signals in the atmosphere over distances of several hundred meters. Several factors contribute to the usefulness of these data: extensive meteorological measurements were made, controlled sources were used, the data were recorded with a high dynamic range digital system that preserved phase information and a significant number of measurement points were obtained allowing both longitudinal and transverse studies. Further, Michigan Tech, in cooperation with the U.S. Army TARDEC, has developed phase tracking algorithms for studying vehicle acoustic signals. These techniques provide an excellent tool for analyzing the amplitude and phase fluctuations of the JAPE data. The results of studies such as those reported here have application at several levels: the mechanisms of signal amplitude and phase fluctuations in propagating acoustic signals are not well understood nor are the mathematical models highly developed, acoustic arrays depend strongly on signal coherence and signal amplitude stability in order to perform to their design specifications and active noise control implementation in regions considerably removed from the primary and secondary sources depends upon signal amplitude and phase stability. Work reported here is preliminary in nature but it does indicate the utility of the phase tracking and amplitude detection algorithms. The results obtained indicate that the phase fluctuations of the JAPE continuous multiple tone data (simultaneous transmission of 80, 200 and 500 Hz) are in general agreement with existing theories but the amplitude fluctuations are seen to be less well behaved and show less consistency

The evolution of depletion zones beneath mud volcanoes

Depletion zones are the least well understood component of mud volcanic systems. They are generally difficult to image using reflection seismic data, and have only rarely been identified and described in the subsurface. This study documents 277 mapped depletion zones in the western Nile Cone, offshore Egypt, of which the dimensions and stratigraphic characteristics of a sub-set of 86 depletion zones associated with mud volcanoes of early Pliocene to Recent age are recorded. The primary database used is a large (4,300 km2) 3D seismic survey in which depletion zones can be confidently interpreted using a set of simple criteria. The sub-set of 86 depletion zones were selected for morphometric analysis by virtue of the quality of seismic imaging. The depletion zones are characterised by circular to elliptical planforms with a bowl or conical geometry. They exhibit truncational stratal relationships with their parent stratigraphic unit, which in this area is the Mid-Late Miocene aged OM2 unit, and which occurs directly beneath the Messinian Evaporites. This geometry implies a top-down formation mechanism. Their diameters and relief range from is 600 m–3300 m, and 100 m–740 m, respectively, with a modest scaling relationship between diameter and relief. Flank angles of bowls and cones range from 11⁰ to 41⁰, with a crudely normal distribution, with median and mean values of 26⁰. A model for the evolution of depletion zones in the study area is based on two previous models developed for single source layer plumbing systems and invokes mobilisation of the source layer by sediment collapse and shear-induced liquefaction following initial seal failure by hydraulic fracturing of the evaporite seal. This mechanism may be more widely applicable to mud volcano systems than currently appreciated