Analysis of β-globin chromatin micro-environment using a novel 3C variant, 4Cv

Copyright: © 2010 Pink et al.Higher order chromatin folding is critical to a number of developmental processes, including the regulation of gene expression. Recently developed biochemical techniques such as RNA TRAP and chromosome conformation capture (3C) have provided us with the tools to probe chromosomal structures. These techniques have been applied to the β-globin locus, revealing a complex pattern of interactions with regions along the chromosome that the gene resides on. However, biochemical and microscopy data on the nature of β-globin interactions with other chromosomes is contradictory. Therefore we developed a novel 4C variant, Complete-genome 3C by vectorette amplification (4Cv), which allows an unbiased and quantitative method to examine chromosomal structure. We have used 4Cv to study the microenvironment of the β-globin locus in mice and show that a significant proportion of the interactions of β-globin are inter-chromosomal. Furthermore, our data show that in the liver, where the gene is active, β-globin is more likely to interact with other chromosomes, compared to the brain where the gene is silent and is more likely to interact with other regions along the same chromosome. Our data suggest that transcriptional activation of the β-globin locus leads to a change in nuclear position relative to the chromosome territory.Ryan Pink is supported by a grant from Action Medical Research; Daniel Caley is supported by a grant from The Dunhill Medical Trust; David Carter is supported by a grant from the British Society for Haematology

Law Enforcement’s Information Sharing Infrastructure: A National Assessment

The September 11 attacks impacted society generally, and law enforcement specifically, in dramatic ways. One of the major trends has been changing expectations regarding criminal intelligence practices among state, local, and tribal (SLT) law enforcement agencies and the need to coordinate intelligence efforts and share information at all levels of government. In fact, enhancing intelligence efforts has emerged as a critical issue for the prevention of all threats and crimes. To date, an increasing number of SLT law enforcement agencies have expanded their intelligence capacity, and there have been fundamental changes in the national, state, and local information sharing infrastructure. Moreover, critical to these expanding information sharing expectations is the institutionalization of fusion centers (FCs). Despite these dramatic changes, an expanding role, and the acknowledgement that local law enforcement intelligence is critical to the prevention and deterrence of threats and crimes, very little research exists that highlights issues related to the intelligence practices of SLT law enforcement agencies and FCs.1 This research describes what agencies are doing to build an intelligence capacity and assesses the state of information sharing among agencies. Specifically, a national survey was developed to examine the experiences of SLT agencies and FCs for building an intelligence capacity as well as to understand critical gaps in the sharing of information regarding intelligence

Complete Integrability of Geodesic Motion in General Kerr-NUT-AdS Spacetimes

We explicitly exhibit n-1 constants of motion for geodesics in the general D-dimensional Kerr-NUT-AdS rotating black hole spacetime, arising from contractions of even powers of the 2-form obtained by contracting the geodesic velocity with the dual of the contraction of the velocity with the (D-2)-dimensional Killing-Yano tensor. These constants of motion are functionally independent of each other and of the D-n+1 constants of motion that arise from the metric and the D-n = [(D+1)/2] Killing vectors, making a total of D independent constants of motion in all dimensions D. The Poisson brackets of all pairs of these D constants are zero, so geodesic motion in these spacetimes is completely integrable.Comment: 4 pages. We have now found that the geodesic motion is not just integrable, but completely integrabl

Intrinsic and extrinsic factors drive ontogeny of early-life at-sea behaviour in a marine top predator

Young animals must learn to forage effectively to survive the transition from parental provisioning to independent feeding. Rapid development of successful foraging strategies is particularly important for capital breeders that do not receive parental guidance after weaning. The intrinsic and extrinsic drivers of variation in ontogeny of foraging are poorly understood for many species. Grey seals (Halichoerus grypus) are typical capital breeders; pups are abandoned on the natal site after a brief suckling phase, and must develop foraging skills without external input. We collected location and dive data from recently-weaned grey seal pups from two regions of the United Kingdom (the North Sea and the Celtic and Irish Seas) using animal-borne telemetry devices during their first months of independence at sea. Dive duration, depth, bottom time, and benthic diving increased over the first 40 days. The shape and magnitude of changes differed between regions. Females consistently had longer bottom times, and in the Celtic and Irish Seas they used shallower water than males. Regional sex differences suggest that extrinsic factors, such as water depth, contribute to behavioural sexual segregation. We recommend that conservation strategies consider movements of young naïve animals in addition to those of adults to account for developmental behavioural changes