33 research outputs found
Finishing the euchromatic sequence of the human genome
The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead
Gene and MicroRNA Expression Responses to Exercise; Relationship with Insulin Sensitivity.
Healthy individuals on the lower end of the insulin sensitivity spectrum also have a reduced gene expression response to exercise for specific genes. The goal of this study was to determine the relationship between insulin sensitivity and exercise-induced gene expression in an unbiased, global manner.Euglycemic clamps were used to measure insulin sensitivity and muscle biopsies were done at rest and 30 minutes after a single acute exercise bout in 14 healthy participants. Changes in mRNA expression were assessed using microarrays, and miRNA analysis was performed in a subset of 6 of the participants using sequencing techniques. Following exercise, 215 mRNAs were changed at the probe level (Bonferroni-corrected P<0.00000115). Pathway and Gene Ontology analysis showed enrichment in MAP kinase signaling, transcriptional regulation and DNA binding. Changes in several transcription factor mRNAs were correlated with insulin sensitivity, including MYC, r=0.71; SNF1LK, r=0.69; and ATF3, r= 0.61 (5 corrected for false discovery rate). Enrichment in the 5'-UTRs of exercise-responsive genes suggested regulation by common transcription factors, especially EGR1. miRNA species of interest that changed after exercise included miR-378, which is located in an intron of the PPARGC1B gene.These results indicate that transcription factor gene expression responses to exercise depend highly on insulin sensitivity in healthy people. The overall pattern suggests a coordinated cycle by which exercise and insulin sensitivity regulate gene expression in muscle
miRNA species changing significantly 30 minutes after exercise.
<p>Data are given as Means ± SEM, units are number of specific reads normalized to total reads for a given sample. All changes shown for log2(fold stimulation) are P < 0.0004 or less (Bonferroni correction to keep family-wise error rate at 0.05).</p><p>miRNA species changing significantly 30 minutes after exercise.</p
Overall design of the study.
<p>The overall sequence of study days is shown above an expanded view of the acute exercise bout. During the acute exercise bout, subjects exercise for a total of 48 minutes, consisting of 4 sets of exercise, each set consisting of 8 minutes at 70% HR max, 2 minutes at 90% HR max, and 2 minutes of rest. A biopsy of the <i>vastus lateralis</i> muscle was taken at 30 minutes after completing the four sets of exercise. Bx, Biopsy; VO<sub>2</sub>, rate of oxygen consumption; HR, Heart Rate.</p
Participant characteristics.
<p>Data are given as Mean ± SEM. The six volunteers in the miRNA subset are included in the total group of 14. Data are shown as Mean ± SEM. Peak heart rate was highest value achieved during the VO<sub>2peak</sub> determination. Maximum predicted heart rate = 220—age.</p><p>*P<0.05,</p><p>**P < 0.01 vs. predicted value.</p><p>Kg, kilograms; mg, milligrams; dL, deciliter; pM, picomolar; FFM, fat free mass; BPM, beats per minute.</p><p>Participant characteristics.</p
A Structural Mass Spectrometry Strategy for the Relative Quantitation of Ligands on Mixed Monolayer-Protected Gold Nanoparticles
An unusual N-terminal deletion of the laminin α3a isoform leads to the chronic granulation tissue disorder laryngo-onycho-cutaneous syndrome
Item does not contain fulltextLaryngo-onycho-cutaneous (LOC or Shabbir) syndrome (OMIM 245660) is an autosomal recessive epithelial disorder confined to the Punjabi Muslim population. The condition is characterized by cutaneous erosions, nail dystrophy and exuberant vascular granulation tissue in certain epithelia, especially conjunctiva and larynx. Genome-wide homozygosity mapping localized the gene to a 2 Mb region on chromosome 18q11.2 with an LOD score of 19.8 at theta=0. This region includes the laminin alpha3 gene (LAMA3), in which loss-of-expression mutations cause the lethal skin blistering disorder Herlitz junctional epidermolysis bullosa. Detailed investigation showed that this gene possesses a further 38 exons (76 exons in total) spanning 318 kb of genomic DNA, and encodes three distinct proteins, designated laminin alpha3a, alpha3b1 and alpha3b2. The causative mutation in 15 families was a frameshift mutation 151insG predicting a stop codon 7 bp downstream in an exon that is specific to laminin alpha3a. This protein is secreted only by the basal keratinocytes of stratified epithelia, implying that LOC is caused by dysfunction of keratinocyte-mesenchymal communication. Surprisingly, the 151insG mutation does not result in nonsense-mediated mRNA decay due to rescue of the transcript by an alternative translation start site 6 exons downstream.
The resultant N-terminal deletion of laminin alpha3a was confirmed by immunoprecipitation of secreted proteins from LOC keratinocytes. These studies show that the laminin alpha3a N-terminal domain is a key regulator of the granulation tissue response, with important implications not only in LOC but in a range of other clinical conditions associated with abnormal wound healing
Deciphering ApoE Genotype-Driven Proteomic and Lipidomic Alterations in Alzheimer’s Disease Across Distinct Brain Regions
Alzheimer’s disease (AD) is a neurodegenerative
disease
with a complex etiology influenced by confounding factors such as
genetic polymorphisms, age, sex, and race. Traditionally, AD research
has not prioritized these influences, resulting in dramatically skewed
cohorts such as three times the number of Apolipoprotein E (APOE)
ε4-allele carriers in AD relative to healthy cohorts. Thus,
the resulting molecular changes in AD have previously been complicated
by the influence of apolipoprotein E disparities. To explore how apolipoprotein
E polymorphism influences AD progression, 62 post-mortem patients
consisting of 33 AD and 29 controls (Ctrl) were studied to balance
the number of ε4-allele carriers and facilitate a molecular
comparison of the apolipoprotein E genotype. Lipid and protein perturbations
were assessed across AD diagnosed brains compared to Ctrl brains,
ε4 allele carriers (APOE4+ for those carrying 1 or 2 ε4s
and APOE4– for non-ε4 carriers), and differences in ε3ε3
and ε3ε4 Ctrl brains across two brain regions (frontal
cortex (FCX) and cerebellum (CBM)). The region-specific influences
of apolipoprotein E on AD mechanisms showcased mitochondrial dysfunction
and cell proteostasis at the core of AD pathophysiology in the post-mortem
brains, indicating these two processes may be influenced by genotypic
differences and brain morphology