Analog-digital simulation of transient-induced logic errors and upset susceptibility of an advanced control system

A simulation study is described which predicts the susceptibility of an advanced control system to electrical transients resulting in logic errors, latched errors, error propagation, and digital upset. The system is based on a custom-designed microprocessor and it incorporates fault-tolerant techniques. The system under test and the method to perform the transient injection experiment are described. Results for 2100 transient injections are analyzed and classified according to charge level, type of error, and location of injection

Processing and characterization of aluminium-based MMCs produced by gas pressure infiltration

International audienceA device has been designed and built for unidirectional infiltration of ceramic preforms with a molten metal. It allows production of Al or Mg alloys reinforced with short or continuous ceramic fibres. The apparatus has been tested for different alloys and preforms by varying the process parameters (infiltration pressure, fibre content, melt superheat, preform preheat and solidification speed). As an example, full infiltration of Al 2 O 3-SAFFIL chopped preforms with an Al-4wt.%Cu-1wt.%Mg-0.5wt.%Ag alloy has been achieved under controlled conditions by using a gas pressure between 1 and 3 MPa. The resulting metal matrix composite has been characterised by microscopical observations and mechanical tests. Measurements of Young's modulus, density, microhardness and mechanical loss show that the optimal process conditions for Al-4wt.%Cu-1wt.%Mg-0.5wt.%Ag-SAFFIL composites are a temperature of 750°C for both preform and melt and the maximum infiltration pressure of 3 MPa. Preliminary tests have shown that the gas pressure infiltration apparatus is also suitable to produce continuous fibre reinforced and hybrid metal matrix composites

1-(4-Chloro­phen­yl)-2-[(3-phenyl­isoquinolin-1-yl)sulfan­yl]ethanone

The title compound, C23H16ClNOS, exhibits dihedral angles of 11.73 (1) and 66.07 (1)°, respectively, between the mean plane of the isoquinoline system and the attached phenyl ring, and between the isoquinoline system and the chloro­phenyl ring. The dihedral angle between the phenyl and chlorophenyl rings is 54.66 (1)°

pVAC-Seq: A genome-guided in silico approach to identifying tumor neoantigens

Cancer immunotherapy has gained significant momentum from recent clinical successes of checkpoint blockade inhibition. Massively parallel sequence analysis suggests a connection between mutational load and response to this class of therapy. Methods to identify which tumor-specific mutant peptides (neoantigens) can elicit anti-tumor T cell immunity are needed to improve predictions of checkpoint therapy response and to identify targets for vaccines and adoptive T cell therapies. Here, we present a flexible, streamlined computational workflow for identification of personalized Variant Antigens by Cancer Sequencing (pVAC-Seq) that integrates tumor mutation and expression data (DNA- and RNA-Seq). pVAC-Seq is available a

Long-term outcomes of punctal cauterization in the management of ocular surface diseases

Purpose: To evaluate the long-term outcomes of surgical occlusion of lacrimal puncta using thermal cautery in the management of ocular surface diseases. Methods: We reviewed medical records of 80 consecutive patients from a single academic center who underwent punctal cauterization. Patient demographics, ocular history, symptoms, and signs of ocular surface diseases pre- and post-cauterization were recorded. Results: A total of 80 patients (171 puncta) were included, with an average age of 59 years and a follow-up duration of 27 months. The most common ocular morbidity was ocular graft-versus-host disease (n = 36), followed by primary keratoconjunctivitis sicca (n = 15). Indications for punctal cauterization included plug loss (n = 51), difficulty in plug fitting (n = 11), plug-related complications (n = 6), recanalization of previous cauterization (n = 7), and severe ocular surface disease requiring permanent punctal closure (n = 4). After punctal cauterization, the percentage of eyes with severe (21%) and moderate (25%) dry eye decreased significantly (8% and 19% at 3 months and 6% and 17% at 12 months, P = 0.0006). Fifty-four percent of patients reported improvement in their symptoms. The rate of recanalization was 21% during the follow-up period. The use of topical corticosteroids was associated with higher recanalization rate. Associated complications were limited to temporary pain and swelling. Conclusions: Punctal cauterization is an effective modality in treating severe ocular surface diseases in patients who repeatedly lose punctal plugs, and it can be easily performed in a clinic setting without major complications. However, cauterization may need to be repeated in up to a quarter of cases because of recanalization.Ophthalmic researc

Diurnal Differences in Intracellular Replication Within Splenic Macrophages Correlates With the Outcome of Pneumococcal Infection

Circadian rhythms affect the progression and severity of bacterial infections including those caused by Streptococcus pneumoniae, but the mechanisms responsible for this phenomenon remain largely elusive. Following advances in our understanding of the role of replication of S. pneumoniae within splenic macrophages, we sought to investigate whether events within the spleen correlate with differential outcomes of invasive pneumococcal infection. Utilising murine invasive pneumococcal disease (IPD) models, here we report that infection during the murine active phase (zeitgeber time 15; 15h after start of light cycle, 3h after start of dark cycle) resulted in significantly faster onset of septicaemia compared to rest phase (zeitgeber time 3; 3h after start of light cycle) infection. This correlated with significantly higher pneumococcal burden within the spleen of active phase-infected mice at early time points compared to rest phase-infected mice. Whole-section confocal microscopy analysis of these spleens revealed that the number of pneumococci is significantly higher exclusively within marginal zone metallophilic macrophages (MMMs) known to allow intracellular pneumococcal replication as a prerequisite step to the onset of septicaemia. Pneumococcal clusters within MMMs were more abundant and increased in size over time in active phase-infected mice compared to those in rest phase-infected mice which decreased in size and were present in a lower percentage of MMMs. This phenomenon preceded significantly higher levels of bacteraemia alongside serum IL-6 and TNF-alpha concentrations in active phase-infected mice following re-seeding of pneumococci into the blood. These data greatly advance our fundamental knowledge of pneumococcal infection by linking susceptibility to invasive pneumococcal infection to variation in the propensity of MMMs to allow persistence and replication of phagocytosed bacteria. These findings also outline a somewhat rare scenario whereby the active phase of an organism's circadian cycle plays a seemingly counterproductive role in the control of invasive infection

Detection of protection benefits for predatory fishes depends on census methodology

Marine protected areas (MPAs) are used as fisheries management and conservation tools. Well-enforced no-take zones allow the rebuilding of natural populations of exploited species; however, there is still controversy on the role of buffer zones. The effectiveness of MPAs could be underestimated, as fish population assessments depend largely on traditional methodologies that have difficulties in detecting predatory fish because of their low abundances, their patchy distribution, and their reaction to the presence of divers. The performance of different census methods was compared in assessing the protection benefits for large predatory fishes under different protection levels (i.e. no-take and buffer zones) in five Mediterranean MPAs. Specifically, conventional strip transects (CSTs, 50 × 5 m2) and tracked roaming transects combined with distance sampling (TRT + DS, variable lengths) were compared, including a series of TRT-derived estimators with variable transect lengths and fixed widths of 20, 10, and 6 m (TRT20, TRT10, and TRT6, respectively). Additionally, the effectiveness of the MPAs studied and protection levels for conserving large predatory species was evaluated. Transects covering larger areas (i.e. TRT + DS and TRT20) allowed the detection of a greater number of species and yielded more accurate estimates of density and biomass than transects of narrower fixed widths, particularly the CSTs, which were associated with the lowest richness detection capability, accuracy, and precision. On average, both no-take zones and buffer zones appeared effective for the conservation of predatory fishes, indicating that multiple protection areas were ecologically effective. Differences between MPAs were also observed, however, probably arising from both local environmental and management factors. We suggest the implementation of methodologies with larger transects for the study of large predatory fish, combined with CSTs for the rest of the fish community, in order to avoid biases in predatory population assessments, which are key indicators of MPA effectiveness

Preclinical transgenic and patient-derived xenograft models recapitulate the radiological features of human adamantinomatous craniopharyngioma

To assess the clinical relevance of transgenic and patient-derived xenograft models of adamantinomatous craniopharyngioma (ACP) using serial magnetic resonance imaging (MRI) and high resolution post-mortem microcomputed tomography (μ-CT), with correlation with histology and human ACP imaging. The growth patterns and radiological features of tumors arising in Hesx1(Cre/+) ;Ctnnb1(lox(ex3)/+) transgenic mice, and of patient-derived ACP xenografts implanted in the cerebral cortex, were monitored longitudinally in vivo with anatomical and functional MRI, and by ex vivo μ-CT at study end. Pathological correlates with hematoxylin and eosin stained sections were investigated. Early enlargement and heterogeneity of Hesx1(Cre/+) ;Ctnnb1(lox(ex3)/+) mouse pituitaries was evident at initial imaging at 8 weeks, which was followed by enlargement of a solid tumor, and development of cysts and hemorrhage. Tumors demonstrated MRI features that recapitulated those of human ACP, specifically, T1 -weighted signal enhancement in the solid tumor component following Gd-DTPA administration, and in some animals, hyperintense cysts on FLAIR and T1 -weighted images. Ex vivo μ-CT correlated with MRI findings and identified smaller cysts, which were confirmed by histology. Characteristic histological features, including wet keratin and calcification, were visible on μ-CT and verified by histological sections of patient-derived ACP xenografts. The Hesx1(Cre/+) ;Ctnnb1(lox(ex3)/+) transgenic mouse model and cerebral patient-derived ACP xenografts recapitulate a number of the key radiological features of the human disease and provide promising foundations for in vivo trials of novel therapeutics for the treatment of these tumors