Caracterización técnica y económica del sistema de producción de búfalos Bubalus bubalis en el departamento de Córdoba de Colombia

The objective of this work was to carry out a descriptive study that would allow generating basic technological and socioeconomic information on the buffalo production systems in the department of Córdoba, Colombia. The collection of information was obtained through a survey form applied to 26 production units. The statistical indicators were tabulated and analyzed with the SPSS program. It can be seen that 63% of the production systems have a dual-purpose vocation where the Murrah race predominates (52%), with production units greater than 100 hectares (77%). 88% carry out a sanitary plan on the farms, 35% of those surveyed reports highest mortality in buffalo calf and reproductive problems are concentrated in abortions (26%). The type of mating is continuous (96%), they expose one male for every 40 females (53%). 96% of the respondents perform rectal palpation at least twice a year. In more than 90% they keep records, most of them use software and maintain control of both the technical aspects and the production and sale of products. In the production costs, the works that participate in 70% of the total, the veterinary drugs 2%, the direct costs participate with 76%. The economic returns for an average farm of 250 hectares present a net income of $209,463,933 COP. The profitability of 32$209.463.933 COP. La rentabilidad de 32% y el punto de equilibrio de 85% del total de la producción, demuestra que este sistema es eficiente y rentabl

Effects of growth hormone on in situ culture of bovine preantral follicles are dose dependent

The objective of this study was to evaluate different concentrations of growth hormone (GH) on the development of bovine preantral follicles cultured included in the ovarian tissue (in situ) on the rates of morphologically normal, viable, primordial and developing follicles, as well as the oocyte and follicle diameter and ultrastructural analysis. Ovarian fragments collected from cows with no cross‐breeds defined were cultured in situ for 1 and 7 days in minimal essential medium (α‐MEM+) supplemented with different concentrations of recombinant human GH (0, 10, 25, 50 ng/ml). The ovarian fragments non‐cultured (control) and cultured were processed for classic histology, mechanical isolation and electron transmission microscopy (MET). The parameters underwent anova (Tukey′s and Dunnett′s tests) and chi‐square test (χ2). After 7 days of culture, the treatment with 50 ng/ml GH showed no differences with fresh control (p > 0.05) and had greater effectiveness than in the 0, 10 and 25 ng/ml GH concentrations of the morphologically normal follicles. Regarding the primordial follicles, a reduction was observed in the 50 ng/ml GH concentration concomitant with the significant increase in developing follicles, differing from both the fresh control and the other GH concentrations tested. In addition, 50 ng/ml GH showed a larger follicle and oocyte diameter when compared to the other treatments cultured. Similar structures were ultrastructurally observed in the control group, 50 ng/ml GH. Follicles cultured in 10 ng/ml GH showed nuclear invagination, vacuoles and lesioned basal membrane. Hence, it is concluded that 50 ng/ml GH is the most effective concentration for the development of preantral follicles cultured in situ

Effects of IGF‐1 on In vitro culture of bovine preantral follicles are dose‐dependent

This study aimed at assessing the effect of different concentrations of the growth factor similar to insulin 1 (IGF‐1) in the development, survival and ultrastructure of the bovine preantral follicles cultured in situ. Fragments of bovine ovarian cortical tissue were cultured during 1 and 7 days in 1 ml of α‐MEM+, supplemented with different concentrations of human recombinant IGF‐1 (0, 30, 70 and 100 ng/ml), in an incubator at 37°C and 5% of CO2 in 24‐well plates with total replacement of the medium every 2 days. Non‐cultured ovarian fragments (control) and ovarian fragments cultured during 1 and 7 days were processed for classic histology, mechanical isolation and electron transmission microscopy (ETM). Parameters such as normality, viability, activation, development, diameter and ultrastructure were evaluated. All statistical analyses were carried out using sas Version 9.2. The results showed that the percentage of follicles morphologically normal in the IGF‐1 30 ng/ml treatment was similar to the fresh control (p > 0.05) both on the day 1 and on the day 7 of in vitro culture. In the viability analysis, the cultured treatments maintained the percentage of viable follicles during the entire culture period (p > 0.05). After 7 days of culture, the IGF‐1 30 ng/ml treatment showed higher percentages of developing follicles (48.33%) than those of the fresh control (22.22%) and the cultured treatments (p < 0.05). Also, after 7 days of culture, IGF‐1 30 ng/ml presented a higher follicular diameter when compared to the control and other concentrations of IGF‐1 tested. Ultrastructurally, the non‐cultured control and IGF‐1 30 ng/ml, after 7 days of culture, showed conserved oocytes, nuclei and organelles. Hence, it is concluded that IGF‐1 30 ng/ml was the most efficient concentration for the development of bovine preantral follicles cultured in vitro