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A taxonomic study in the genus Hackelia in western North America
A biosystematic study was made on the species of Hackelia of
northwest America and adjacent Canada. During the course of the
study 140 populations, representing all species and varieties, were
transplanted to the greenhouse for study under controlled conditions.
Chromosome counts were made for all 14 taxa studied, and intra- and
interspecific hybridizations were attempted. For the study over
3000 specimens were borrowed from 21 herbaria in the United States
and Canada.
Only two chromosome levels were found: 2n=24 and 2n=48.
Chromosome counts were obtained only from mitotic cell divisions,
as some difficulty was encountered in making usable pollen-mother
cell smears. This was especially true in the tetraploids, where
normal chemical techniques for fixation of buds resulted in the
chromosomes tending to stick together. Because none of the species
have chromosome numbers higher than tetraploid, it was felt that
polyploidy, although present, has not been a dominant evolutionary
mechanism in this section of the genus. The tetraploid taxa under
study probably evolved from one or two original tetraploid ancestors
which underwent speciation during the Quaternary period.
It was found that nutlet features were of limited value as
taxonomic characters in some species of Hackelia. The nutlets of
some species are highly variable with regard to surface texture,
number and size of intramarginal prickles, and the length and degree
of fusion of the marginal prickles, The features of the fornix
of the corolla are also variable and must be used with care.
The distinctness of the tetraploid taxa is maintained in part
by their specific habitat requirements. They occur principally
in open sites with rocky to sandy soil, where plant competition is
minimal.
An artificial key to the species and varieties is presented in
the taxonomy section, together with complete synonymies, descriptions,
lists of cited specimens, and maps of distribution.
One new species is described from southeastern Oregon (Hackelia
ophiobia) and one new variety from central Washington (H. hispida
var. disjuncta), Hackelia diffusa (Lehm.) Johnst. is defined to
include var. diffusa, var.arida (=H. arida (Piper) Johnst.) and var.
cottonii (=H. cottonii (Piper) Brand)
Histopathologic and Biochemical Responses in Arctic Marine Bivalve Molluscs Exposed to Experimentally Spilled Oil
Following two experimental spills of chemically dispersed and undispersed crude oil in shallow bays on the northwest coast of Baffin Island, Canadian Arctic, the bivalve molluscs Mya truncata and Macoma calcarea accumulated significant amounts of petroleum hydrocarbons in bays receiving dispersed oil and in those receiving crude oil alone (Boehm et all., 1987). Following the spills, Mya released accumulated hydrocarbons more rapidly than Macoma. ... The results of the biochemical analyses indicate that Mya from the four bays were not severely stressed by either dispersed oil or oil alone. Immediately after the spill, clams from the dispersal oil bays were nearly normal, while those from the bay receiving oil alone appeared stressed. These results seem to corroborate results from analytical chemistry and histopathology: that the acute effects of dispersed oil are greater than those of undispersed oil, but effects of undispersed oil on infaunal molluscs develop more slowly and persist longer than those from dispersed oil.Key words: oil spill, dispersant, Mya truncata, Macoma calcarea, histopathology, biochemistry, neoplasia, free amino acids, glycogen, parasitesMots clés: marée noire, agent de dispersion, Mya truncata, Macoma calcarea, histopathologie, biochimie, néoplasme, acides aminés libres, glycogène, parasite
Information Services for Agriculture: The Role of Technology
Significant improvements in technology-supported information services
have created opportunities for their utilization by the farmers and ranchers
of our Nation. This report highlights the development and expanded offering
of these systems, describes current operational and experimental systems, and
presents salient legislative initiatives which address this priority area
Metabolic Feedback Inhibition Influences Metabolite Secretion by the Human Gut Symbiont Bacteroides thetaiotaomicron
Microbial metabolism and trophic interactions between microbes give rise to complex multispecies communities in microbe-host systems. Bacteroides thetaiotaomicron (B. theta) is a human gut symbiont thought to play an important role in maintaining host health. Untargeted nuclear magnetic resonance metabolomics revealed B. theta secretes specific organic acids and amino acids in defined minimal medium. Physiological concentrations of acetate and formate found in the human intestinal tract were shown to cause dose-dependent changes in secretion of metabolites known to play roles in host nutrition and pathogenesis. While secretion fluxes varied, biomass yield was unchanged, suggesting feedback inhibition does not affect metabolic bioenergetics but instead redirects carbon and energy to CO2 and H2. Flux balance analysis modeling showed increased flux through CO2-producing reactions under glucose-limiting growth conditions. The metabolic dynamics observed for B. theta, a keystone symbiont organism, underscores the need for metabolic modeling to complement genomic predictions of microbial metabolism to infer mechanisms of microbemicrobe and microbe-host interactions
Evaluation of aldehyde dehydrogenase 1 promoter polymorphisms identified in human populations
BACKGROUND: Cytosolic aldehyde dehydrogenase, or ALDH1A1, functions in ethanol detoxification, metabolism of neurotransmitters, and synthesis of retinoic acid. Because the promoter region of a gene can influence gene expression, the ALDH1A1 promoter regions were studied to identify polymorphism, to assess their functional significance, and to determine whether they were associated with a risk for developing alcoholism.
METHODS: Sequence analysis was performed in the promoter region by using Asian, Caucasian, and African American subjects. The resulting polymorphisms were assessed for frequency in Asian, Caucasian, Jewish, and African American populations and tested for associations with alcohol dependence in Asian and African American populations of alcoholics and controls. The functional significance of each polymorphism was determined through in vitro expression analysis by using HeLa and HepG2 cells.
RESULTS: Two polymorphisms, a 17 base pair (bp) deletion (-416/-432) and a 3 bp insertion (-524), were discovered in the ALDH1A1 promoter region: ALDH1A1*2 and ALDH1A1*3, respectively. ALDH1A1*2 was observed at frequencies of 0.035, 0.023, 0.023, and 0.012 in the Asian, Caucasian, Jewish, and African American populations, respectively. ALDH1A1*3 was observed only in the African American population, at a frequency of 0.029. By using HeLa and HepG2 cells for in vitro expression, the activity of the luciferase reporter gene was significantly decreased after transient transfection of ALDH1A1*3-luciferase compared with the wild-type construct ALDH1A1*1-luciferase. In an African American population, a trend for higher frequencies of the ALDH1A1*2 and ALDH1A1*3 alleles was observed in a population of alcoholics (p = 0.03 and f = 0.12, respectively) compared with the control population.
CONCLUSIONS: ALDH1A1*2 and ALDH1A1*3 may influence ALDH1A1 gene expression. Both ALDH1A1*2 and ALDH1A1*3 produce a trend in an African American population that may be indicative of an association with alcoholism; however, more samples are required to validate this observation. The underlying mechanisms contributing to these trends are still unknown
A questionnaire elicitation of surgeons' belief about learning within a surgical trial
PMID: 23145113 [PubMed - indexed for MEDLINE] PMCID: PMC3493499 Free PMC ArticlePeer reviewedPublisher PD
Tick Haller\u27s Organ, a New Paradigm for Arthropod Olfaction: How Ticks Differ from Insects
Ticks are the vector of many human and animal diseases; and host detection is critical to this process. Ticks have a unique sensory structure located exclusively on the 1st pairs of legs; the fore-tarsal Haller\u27s organ, not found in any other animals, presumed to function like the insect antennae in chemosensation but morphologically very different. The mechanism of tick chemoreception is unknown. Utilizing next-generation sequencing and comparative transcriptomics between the 1st and 4th legs (the latter without the Haller\u27s organ), we characterized 1st leg specific and putative Haller\u27s organ specific transcripts from adult American dog ticks, Dermacentor variabilis. The analysis suggested that the Haller\u27s organ is involved in olfaction, not gustation. No known odorant binding proteins like those found in insects, chemosensory lipocalins or typical insect olfactory mechanisms were identified; with the transcriptomic data only supporting a possible olfactory G-protein coupled receptor (GPCR) signal cascade unique to the Haller\u27s organ. Each component of the olfactory GPCR signal cascade was identified and characterized. The expression of GPCR, Gαo and ÎČ-arrestin transcripts identified exclusively in the 1st leg transcriptome, and putatively Haller\u27s organ specific, were examined in unfed and blood-fed adult female and male D. variabilis. Blood feeding to repletion in adult females down-regulated the expression of all three chemosensory transcripts in females but not in males; consistent with differences in post-feeding tick behavior between sexes and an expected reduced chemosensory function in females as they leave the host. Data are presented for the first time of the potential hormonal regulation of tick chemosensation; behavioral assays confirmed the role of the Haller\u27s organ in N,N-diethyl-meta-toluamide (DEET) repellency but showed no role for the Haller\u27s organ in host attachment. Further research is needed to understand the potential role of the GPCR cascade in olfaction
Coherent Signal Amplification in Bistable Nanomechanical Oscillators by Stochastic Resonance
Stochastic resonance is a counter-intuitive concept[1,2], ; the addition of
noise to a noisy system induces coherent amplification of its response. First
suggested as a mechanism for the cyclic recurrence of ice ages, stochastic
resonance has been seen in a wide variety of macroscopic physical systems:
bistable ring lasers[3], SQUIDs[4,5], magnetoelastic ribbons[6], and
neurophysiological systems such as the receptors in crickets[7] and
crayfish[8]. Although it is fundamentally important as a mechanism of coherent
signal amplification, stochastic resonance is yet to be observed in nanoscale
systems. Here we report the observation of stochastic resonance in bistable
nanomechanical silicon oscillators, which can play an important role in the
realization of controllable high-speed nanomechanical memory cells. Our
nanomechanical systems were excited into a dynamic bistable state and modulated
in order to induce controllable switching; the addition of white noise showed a
marked amplification of the signal strength. Stochastic resonance in
nanomechanical systems paves the way for exploring macroscopic quantum
coherence and tunneling, and controlling nanoscale quantum systems for their
eventual use as robust quantum logic devices.Comment: 18 pages, 4 figure
Limits on the cosmological abundance of supermassive compact objects from a millilensing search in gamma-ray burst data
A new search for the gravitational lens effects of a significant cosmological
density of supermassive compact objects (SCOs) on gamma-ray bursts has yielded
a null result. We inspected the timing data of 774 BATSE-triggered GRBs for
evidence of millilensing: repeated peaks similar in light-curve shape and
spectra. Our null detection leads us to conclude that, in all candidate
universes simulated, is favored for , while in some universes and mass ranges the density
limits are as much as 10 times lower. Therefore, a cosmologically significant
population of SCOs near globular cluster mass neither came out of the
primordial universe, nor condensed at recombination.Comment: 14 pages including 3 figures, appeared 2001 January 2
Microwave Spectroscopy
Contains research objectives and reports on one research project.Signal Corps Contract DA-36-039-sc-8737
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