in situ experimental tests on masonry panels strengthened with textile reinforced mortar composites

Abstract Textile Reinforced Mortar (TRM) composites are a retrofitting techniques used for strengthening masonry structures. The system is composed of dry fibers grids embedded in two layers of inorganic matrix. The paper describes the results of an in-situ experimental campaign on ancient masonry panels reinforced with different TRM systems. The tests were performed in a building located in Finale Emilia (north of Italy) built at the beginning of the last century. Four diagonal compressive tests were performed on unreinforced and reinforced walls. The walls were strengthened with different configurations: two panels were reinforced with a TRM systems composed of a lime mortar and two different types of glass fiber grids and twist steel bars used as anchors; one panel was reinforced with a layer of TRM on one side and a Near Surface Mounted (NSM) system on the other one. The results of the tests are described and a complete mechanical characterization of the reinforcement systems and of the masonry was performed to analyze the experimental results and validate simple analytical models

Combined Analysis of HPV DNA and p16INK4a Expression to Predict Prognosis in ASCUS and LSIL Pap Smears

Human papillomavirus (HPV) is known to play an important etiological role in the genesis of cervical cancer, but only a very small proportion of infected women develop invasive cervical cancer. The purpose of cervical cancer prevention is early diagnosis of its precursors. The molecular detection of HPV DNA as a diagnostic test to cervical carcinogenesis gave a low positive predictive value as compared to the use of biomarkers. p16INK4a has been proposed as putative surrogate biomarkers that would allow identification of dysplastic cervical epithelia. Serial consecutive cervical smears were test for high-risk HPV, stained with immunocytochemistry for p16INK4a and followed-up for 36 months. The aim of the study was to evaluate the immunohistochemical expression of p16INK4a as a marker of progression risk in low-grade dysplastic lesions of the cervix uteri. In the present series, significant p16 overexpression was observed in the group that progressed from low to high-grade squamous intraepithelial lesion when compared with the group that did not progress. In conclusion, overexpression of p16INK4a acts as potential biomarkers for cervical cancer progression from premalignant lesions

new italian guidelines for design of externally bonded fabric reinforced cementitious matrix frcm systems for repair and strengthening of masonry and concrete structures

Abstract The paper summarizes the main features of a standardization activity carried out in Italy by the Ministry of Public Works, to which two of the authors have taken part, for the homologation and the acceptance of Fabric-Reinforced Cementitious Matrix (FRCM) composites. During the last years, such composite materials have becoming increseangly popular in the civil engineering field for strengthening existing constructions, even if difficulties can occur in their mechanical characterization that is strongly affected by different and complex failure mechanisms. The American ACI 549.4R-13 is currently the only available guideline for design and construction of these systems. In this framework, the paper describes the Italian proposals for the homologation process of FRCM materials as well as for the design of strengthening interventions with these composites. Comparisons with the American guideline are also reported together with some considerations regarding the different partial safety factors

Ancient masonry arches and vaults strengthened with TRM, SRG and FRP composites: Numerical analyses

[EN] The two arches and the three vaults experimentally described in Carozzi et al. (2017) are here analyzed with a novel robust FE lower bound limit analysis code, suitable to predict active failure mechanisms, lines of thrust and collapse loads in absence and presence of TRM, SRG and FRP reinforcement. The approach relies into a discretization into rigid-infinitely resistant quadrilateral elements for masonry, interfaces between contiguous elements exhibiting limited strength and perfectly bonded rigid-plastic trusses representing the reinforcement. For masonry, a No Tension Material NTM model can be adopted to compare with classic Heyman¿s results, but also a limited compressive and tensile strength with a cohesive frictional behavior in shear may be accounted for in a relatively simple fashion, i.e. in principle with the possibility to model shear sliding and compression crushing. Debonding and delamination of the reinforcement are considered in a conventional way, assuming trusses with a limited tensile strength derived from either experimental data available or consolidated formulas from the literature. With the knowledge of the exact position of the hinges provided by limit analysis, 2D FE static analyses with non-linearity and softening concentrated exclusively on hinges are carried out, to simply extend the knowledge beyond collapse loads estimation towards a prediction of initial stiffness and ultimate displacements. In all cases, promising agreement with experiments is observed.Part of the analyses were developed within the activities of Rete dei Laboratori Universitari di Ingegneria Sismica - ReLUIS for the research program funded by the Dipartimento di Protezione Civile.Bertolesi, E.; Milani, G.; Carozzi, FG.; Poggi, C. (2018). Ancient masonry arches and vaults strengthened with TRM, SRG and FRP composites: Numerical analyses. Composite Structures. 187:385-402. https://doi.org/10.1016/j.compstruct.2017.12.021S38540218

Evaluation of bivalent human papillomavirus (HPV) vaccine safety and tolerability in a sample of 25 year old Tuscan women

The aim of this study was to gather data on the safety of the HPV-16/18 AS04-adjuvated vaccine among women aged 25, evaluating the frequency and severity of adverse events reported after vaccination and to compare the results obtained with previously published data regarding a sample of Italian preadolescents. Every woman residing in the province of Florence and in the age group targeted by the cervical cancer screening was invited to participate. Participants registered daily, for 14 d post-vaccination, solicited local and systemic reactions, as well as unsolicited adverse events in a developed ad hoc safety diary card. Data were collected in a database in Access and analyzed using STATA 11 SE statistical software. A total of 271 participants were recruited in the study group. All three diary cards were completed and delivered by 186 subjects (85.7% of participants). In all, a total of 616 diary cards were collected: 216 after the 1st dose, 209 after the 2nd dose and 191 after the 3rd dose. No severe symptoms were registered. The most frequently reported adverse reaction proved to be pain at the site of injection (83.4% of doses), followed by local swelling (20.8%) and pyrexia (14.6%). The safety and tolerability of the HPV-16/18 AS04-adjuvated vaccine in this sample of adult women aged 25 did not differ much from that previously observed in a sample of preadolescents Italian girls. Fever and local pain were however more frequently registered in our sample of adult women

Concurrent infections with multiple human papillomavirus (HPV) types in the New Technologies for Cervical Cancer (NTCC) screening study

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Human papillomavirus infection and risk factors in a cohort of Tuscan women aged 18-24: results at recruitment

<p>Abstract</p> <p>Background</p> <p>There is conclusive evidence that human papillomavirus (HPV) infections of the cervix are a necessary cause of cervical cancer. In Italy there are consistent data of HPV prevalence in women aged 25 - 64 years, but there is limited data for younger women. The objective of this on-going 3-year prospective cohort study is to investigate the prevalence, acquisition, clearance and persistence of HPV infections in young Tuscan women and the risk factors correlated with such events.</p> <p>Methods</p> <p>One thousand and sixty-six women aged between 18 and 24 years were enrolled and received an initial HPV test. They were asked to return to the clinic over the study period for further tests every 12 months, if their HPV HR result was negative, or every 6 months, if positive. Additionally, women with an HPV positive result were given a cytological examination and if the cytological diagnosis was ASC-US or more severe, only women with HPV HR, were referred for colposcopy.</p> <p>Results</p> <p>We present here data for the enrolment phase of the study. At baseline, within the study sample, just under 30% of women were infected by HPV and 19.3% of women were infected with oncogenic types. A relationship was highlighted between HPV infection, number of sexual partners (in particularly in the last 3 years) and the lifetime number of partner's partners. Condom use showed a slight protective effect in univariate analysis but these data were not statistically significant in multivariate analysis. The association between HPV infection and demographic and behavioural variables were tested by crude odds ratio (OR). Multivariate logistic regression was applied to compute the adjusted odds ratios.</p> <p>Conclusions</p> <p>The prevalence of oncogenic HPV types was high in young Tuscan women. The 3-year follow-up of this cohort may provide a better understanding of the processes of acquisition, clearance and persistence of infection and the correlated risk factors.</p

Givinostat-Liposomes: Anti-Tumor Effect on 2D and 3D Glioblastoma Models and Pharmacokinetics

Glioblastoma is the most common and aggressive brain tumor, associated with poor prognosis and survival, representing a challenging medical issue for neurooncologists. Dysregulation of histone-modifying enzymes (HDACs) is commonly identified in many tumors and has been linked to cancer proliferation, changes in metabolism, and drug resistance. These findings led to the development of HDAC inhibitors, which are limited by their narrow therapeutic index. In this work, we provide the proof of concept for a delivery system that can improve the in vivo half-life and increase the brain delivery of Givinostat, a pan-HDAC inhibitor. Here, 150-nm-sized liposomes composed of cholesterol and sphingomyelin with or without surface decoration with mApoE peptide, inhibited human glioblastoma cell growth in 2D and 3D models by inducing a time-and dose-dependent reduction in cell viability, reduction in the receptors involved in cholesterol metabolism (from −25% to −75% of protein levels), and reduction in HDAC activity (−25% within 30 min). In addition, liposome-Givinostat formulations showed a 2.5-fold increase in the drug half-life in the bloodstream and a 6-fold increase in the amount of drug entering the brain in healthy mice, without any signs of overt toxicity. These features make liposomes loaded with Givinostat valuable as potential candidates for glioblastoma therapy

Multimodal lung cancer screening using the ITALUNG Biomarker Panel and Low Dose Computed Tomography. Results of the ITALUNG biomarker study

Asymptomatic high-risk subjects, randomized in the intervention arm of the ITALUNG trial (1406 screened for lung cancer), were enrolled for the ITALUNG biomarker study (n = 1356), in which samples of blood and sputum were analysed for plasma DNA quantification (cut off 5ng/ml), loss of heterozygosity and microsatellite instability. The ITALUNG biomarker panel (IBP) was considered positive if at least one of the two biomarkers included in the panel was positive. Subjects with and without lung cancer diagnosis at the end of the screening cycle with LDCT (n = 517) were evaluated. Out of 18 baseline screen detected lung cancer cases, 17 were IBP positive (94%). Repeat screen-detected lung cancer cases were 18 and 12 of them positive at baseline IBP test (66%). Interval cancer cases (2-years) and biomarker tests after a suspect Non Calcific Nodule follow-up were investigated. The single test versus multimodal screening measures of accuracy were compared in a simulation within the screened ITALUNG intervention arm, considering screen-detected and interval cancer cases. Sensitivity was 90% at baseline screening. Specificity was 71%% and 61% for LDCT and IBP as baseline single test, and improved at 89% with multimodal, combined screening. The positive predictive value was 4.3% for LDCT at baseline and 10.6% for multimodal screening. Multimodal screening could improve the screening efficiency at baseline and strategies for future implementation are discussed. If IBP was used as primary screening test, the LDCT burden might decrease of about 60%

A feasibility study of the use of the AutoPap screening system as a primary screening and location-guided rescreening device

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