Pintura y lectura: La herencia centenaria del proyecto cultural de Vasconcelos

ArtículoLa investigación muestra los inicios de la alfabetización en un contexto posrevolucionario, donde era notorio que en el terreno de la cultura, la educación y la lectura había desigualdades e injusticias para una sociedad con población mayoritariamente analfabeta y con poca cultura plástica visual. Se presenta también un breve panorama de la gestión de José Vasconcelos al frente de la recién creada Secretaría de Educación Pública (SEP) y su ambiciosa campaña cultural. A partir de esto se realiza un análisis de las similitudes y diferencias entre los lenguajes escrito y pictórico que promovió Vasconcelos hace un siglo y que, a pesar de los avatares, siguen vigentes y, además de mantener su función educativa primigenia, cumplen con otros usos. De esta manera se contribuye con apuntes para la configuración de la historia conjunta de la pintura y la lectura en México y su establecimiento como discurso histórico-identitario-nacionalista textual y visual. Así, estos apuntes suman elementos para los estudios históricos sobre la pintura y la lectura que hacen visibles los sucesos, las rupturas o las continuidades de un proyecto cultural nacional próximo a cumplir sus primeros cien años.Propio

Antimicrobial effect of silk and catgut suture threads coated with biogenic silver nanoparticles

Abstract Two bionanocomposites based on suture threads, silk-silver nanoparticles (Ag NPs) and catgut-Ag NPs, were prepared through a green chemistry methodology using Chenopodium ambrosioides (Mexican Epazote) as reducing agent. UV-Vis spectrophotometry (UV-Vis), Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM), were used for their characterization. UV-Vis confirmed the synthesis of silver nanoparticles. Micrographs showed polydisperse, mostly spherical, Ag NPs attached to both suture threads. The bionanocomposites antimicrobial properties were evaluated through cultures and inhibition zones tests. The Chenopodium ambrosioides- assisted synthesized bionanocomposites have proved antibacterial effect against S. aureus and E. coli in both sutures (silk and catgut) and could be potentially useful for oral or periodontal surgery. There was no significant difference statistically in inhibition of Staphylococcus aureus versus Escherichia coli

Antibacterial effect of silver nanoparticles versus chlorhexidine against streptococcus mutans and lactobacillus casei

Abstract The porpoise of the study was to evaluate the antibacterial effect of silver nanoparticles (Ag-NPs) versus chlorhexidine (CHX) against Streptococcus mutans and Lactobacillus casei. Three different reducing agents were used for the synthesis and characterization of Ag-NPs: sodium borohydride (NaBH4), a chemical method, and Heterotheca inuloides (Hi) and Camellia sinensis (Cs), two eco-friendly methods. The synthesized substance was deposited on deciduous teeth. Its behavior in dental tissues was evaluated through an energy dispersive X-ray spectroscopy (EDS) analysis, using a scanning electron microscope (SEM). The characterization of Ag-NPs in terms of shape, size, and polydispersity was performed through spectrophotometry of ultraviolet-visible light analysis (UV-vis), as well as by transmission electron microscopy. Isolation and culture of strains S. mutans and L. casei were done to perform the microbiological analysis. In Petri dishes, paper discs containing different concentrations of Ag-NPs (synthesized by Hi, and by Cs) were deposited and tested along with paper discs containing CHX. Their antibacterial effect against both bacteria was evaluated by the inhibition zones test. By means of UV-Vis and TEM analysis, it was possible to observe that Heterotheca inuloides produced smaller and more stable nanoparticles, also in greater quantities (17.5 nm), when compared to Camellia sinensis. EDS analysis through SEM showed a 6.25 average absorption of silver in dental tissues. The microbiological analysis revealed a greater zone of inhibition when the test bacteria were in contact with 20 μl of Ag-NPs, synthesized by Hi, being statistically significant (p < 0.05), compared to the growth inhibition zones produced by Cs, and CHX against both strains. We can conclude that eco-friendly methods produced Ag-NPs with an important antibacterial effect in both strains

Antibacterial Effect of Silver Nanoparticles Versus Chlorhexidine Against Streptococcus mutans and Lactobacillus casei

The porpoise of the study was to evaluate the antibacterial effect of silver nanoparticles (Ag-NPs) versus chlorhexidine (CHX) against Streptococcus mutans and Lactobacillus casei. Three different reducing agents were used for the synthesis and characterization of Ag-NPs: sodium borohydride (NaBH4), a chemical method, and Heterotheca inuloides (Hi) and Camellia sinensis (Cs), two eco-friendly methods. The synthesized substance was deposited on deciduous teeth. Its behavior in dental tissues was evaluated through an energy dispersive X-ray spectroscopy (EDS) analysis, using a scanning electron microscope (SEM). The characterization of Ag-NPs in terms of shape, size, and polydispersity was performed through spectrophotometry of ultraviolet-visible light analysis (UV-vis), as well as by transmission electron microscopy. Isolation and culture of strains S. mutans and L. casei were done to perform the microbiological analysis

A Community-Led Central Kitchen Model for School Feeding Programs in the Philippines: Learnings for Multisectoral Action for Health

In devolved governments like the Philippines, local government units (LGUs) must be engaged to develop and coordinate responses to tackle the multisectoral problem of childhood undernutrition. However, current Philippine nutrition interventions, such as decentralized school feeding programs (SFPs), generally rely on the national government, public school teachers, or the private sector for implementation, with mixed results. The central kitchen model for SFPs was developed by 2 Philippine nongovernmental organizations and facilitated large-scale in-school feeding through community multisectoral action. This case study documented coordination processes in February 2018 for 1 urban city and 1 rural province-the model\u27s earliest large-scale implementation sites-that contributed to its institutionalization and sustainability. Data from 24-hour dietary recalls with 308 rural and 310 urban public school students and household surveys with their caregivers showed undernutrition was an urgent problem. Enabling factors and innovative local solutions were explored in focus group discussions with 160 multisector participants and implementers in health care, education, and government, as well as volunteers, parents, and central kitchen staff. The locally led and operated central kitchens promoted community ownership by embedding volunteer pools in social networks and spurring demand for related social services from their LGU. With the LGU as the face of implementation, operations were sustained despite political leadership changes, fostering local government stewardship over nutrition. Leveraging national legislation and funding for SFPs and guided by the Department of Education\u27s standards for SFP eligibility, LGUs had room to adapt the model to local needs. Central kitchens afforded opportunities for scale-up and flexibility that were utilized during natural disasters and the coronavirus disease (COVID-19) pandemic. The case demonstrated empowering civil society can hold volunteers, local implementers, and local governments accountable for multisectoral action in decentralized settings. The model may serve as a template for how other social services can be scaled and implemented in devolved settings

Live Attenuated African Swine Fever Viruses as Ideal Tools to Dissect the Mechanisms Involved in Cross-Protection

African swine fever (ASF) has become the major threat for the global swine industry. Furthermore, the epidemiological situation of African swine fever virus (ASFV) in some endemic regions of Sub-Saharan Africa is worse than ever, with multiple virus strains and genotypes currently circulating in a given area. Despite the recent advances on ASF vaccine development, there are no commercial vaccines yet, and most of the promising vaccine prototypes available today have been specifically designed to fight the genotype II strains currently circulating in Europe, Asia, and Oceania. Previous results from our laboratory have demonstrated the ability of BA71∆CD2, a recombinant LAV lacking CD2v, to confer protection against homologous (BA71) and heterologous genotype I (E75) and genotype II (Georgia2007/01) ASFV strains, both belonging to same clade (clade C). Here, we extend these results using BA71∆CD2 as a tool trying to understand ASFV cross-protection, using phylogenetically distant ASFV strains. We first observed that five out of six (83.3%) of the pigs immunized once with 106 PFU of BA71∆CD2 survived the tick-bite challenge using Ornithodoros sp. soft ticks naturally infected with RSA/11/2017 strain (genotype XIX, clade D). Second, only two out of six (33.3%) survived the challenge with Ken06.Bus (genotype IX, clade A), which is phylogenetically more distant to BA71∆CD2 than the RSA/11/2017 strain. On the other hand, homologous prime-boosting with BA71∆CD2 only improved the survival rate to 50% after Ken06.Bus challenge, all suffering mild ASF-compatible clinical signs, while 100% of the pigs immunized with BA71∆CD2 and boosted with the parental BA71 virulent strain survived the lethal challenge with Ken06.Bus, without almost no clinical signs of the disease. Our results confirm that cross-protection is a multifactorial phenomenon that not only depends on sequence similarity. We believe that understanding this complex phenomenon will be useful for designing future vaccines for ASF-endemic areas.info:eu-repo/semantics/publishedVersio

An Innovative AI-based primer design tool for precise and accurate detection of SARS-CoV-2 variants of concern

As the COVID-19 pandemic winds down, it leaves behind the serious concern that future, even more disruptive pandemics may eventually surface. One of the crucial steps in handling the SARS-CoV-2 pandemic was being able to detect the presence of the virus in an accurate and timely manner, to then develop policies counteracting the spread. Nevertheless, as the pandemic evolved, new variants with potentially dangerous mutations appeared. Faced by these developments, it becomes clear that there is a need for fast and reliable techniques to create highly specific molecular tests, able to uniquely identify VOCs. Using an automated pipeline built around evolutionary algorithms, we designed primer sets for SARS-CoV-2 (main lineage) and for VOC, B.1.1.7 (Alpha) and B.1.1.529 (Omicron). Starting from sequences openly available in the GISAID repository, our pipeline was able to deliver the primer sets for the main lineage and each variant in a matter of hours. Preliminary in-silico validation showed that the sequences in the primer sets featured high accuracy. A pilot test in a laboratory setting confirmed the results: the developed primers were favorably compared against existing commercial versions for the main lineage, and the specific versions for the VOCs B.1.1.7 and B.1.1.529 were clinically tested successfully

Classification and specific primer design for accurate detection of SARS-CoV-2 using deep learning

In this paper, deep learning is coupled with explainable artificial intelligence techniques for the discovery of representative genomic sequences in SARS-CoV-2. A convolutional neural network classifier is first trained on 553 sequences from the National Genomics Data Center repository, separating the genome of different virus strains from the Coronavirus family with 98.73% accuracy. The network’s behavior is then analyzed, to discover sequences used by the model to identify SARS-CoV-2, ultimately uncovering sequences exclusive to it. The discovered sequences are validated on samples from the National Center for Biotechnology Information and Global Initiative on Sharing All Influenza Data repositories, and are proven to be able to separate SARS-CoV-2 from different virus strains with near-perfect accuracy. Next, one of the sequences is selected to generate a primer set, and tested against other state-of-the-art primer sets, obtaining competitive results. Finally, the primer is synthesized and tested on patient samples (n = 6 previously tested positive), delivering a sensitivity similar to routine diagnostic methods, and 100% specificity. The proposed methodology has a substantial added value over existing methods, as it is able to both automatically identify promising primer sets for a virus from a limited amount of data, and deliver effective results in a minimal amount of time. Considering the possibility of future pandemics, these characteristics are invaluable to promptly create specific detection methods for diagnostics

A crowdsourcing database for the copy-number variation of the Spanish population

Background: Despite being a very common type of genetic variation, the distribution of copy-number variations (CNVs) in the population is still poorly understood. The knowledge of the genetic variability, especially at the level of the local population, is a critical factor for distinguishing pathogenic from non-pathogenic variation in the discovery of new disease variants. Results: Here, we present the SPAnish Copy Number Alterations Collaborative Server (SPACNACS), which currently contains copy number variation profiles obtained from more than 400 genomes and exomes of unrelated Spanish individuals. By means of a collaborative crowdsourcing effort whole genome and whole exome sequencing data, produced by local genomic projects and for other purposes, is continuously collected. Once checked both, the Spanish ancestry and the lack of kinship with other individuals in the SPACNACS, the CNVs are inferred for these sequences and they are used to populate the database. A web interface allows querying the database with different filters that include ICD10 upper categories. This allows discarding samples from the disease under study and obtaining pseudo-control CNV profiles from the local population. We also show here additional studies on the local impact of CNVs in some phenotypes and on pharmacogenomic variants. SPACNACS can be accessed at: http://csvs.clinbioinfosspa.es/spacnacs/. Conclusion: SPACNACS facilitates disease gene discovery by providing detailed information of the local variability of the population and exemplifies how to reuse genomic data produced for other purposes to build a local reference database