Overview: Exobiology in solar system exploration

In Aug. 1988, the NASA Ames Research Center held a three-day symposium in Sunnyvale, California, to discuss the subject of exobiology in the context of exploration of the solar system. Leading authorities in exobiology presented invited papers and assisted in setting future goals. The goals they set were as follows: (1) review relevant knowledge learned from planetary exploration programs; (2) detail some of the information that is yet to be obtained; (3) describe future missions and how exobiologists, as well as other scientists, can participate; and (4) recommend specific ways exobiology questions can be addressed on future exploration missions. These goals are in agreement with those of the Solar System Exploration Committee (SSEC) of the NASA Advisory Council. Formed in 1980 to respond to the planetary exploration strategies set forth by the Space Science Board of the National Academy of Sciences' Committee on Planetary and Lunar Exploration (COMPLEX), the SSEC's main function is to review the entire planetary program. The committee formulated a long-term plan (within a constrained budget) that would ensure a vital, exciting, and scientifically valuable effort through the turn of the century. The SSEC's goals include the following: determining the origin, evolution, and present state of the solar system; understanding Earth through comparative planetology studies; and revealing the relationship between the chemical and physical evolution of the solar system and the appearance of life. The SSEC's goals are consistent with the over-arching goal of NASA's Exobiology Program, which provides the critical framework and support for basic research. The research is divided into the following four elements: (1) cosmic evolution of the biogenic compounds; (2) prebiotic evolution; (3) origin and early evolution of life; and (4) evolution of advanced life

Space Station Gas-Grain Simulation Facility: Microgravity Particle Research

A wide variety of experiments significant to Exobiology, Planetary Science, Astrophysics, Atmospheric Science, and basic Chemistry and Physics involves the physical interactions of small particles (micrometer to centimeter in size). In many astro-geophysical systems (atmospheric clouds, interstellar clouds, planetary rings, Titan\u27s organic aerosols, Martian dust storms, lightning, etc.), processes involving small particles determine the overall behavior of the system. Condensation of particles from a gas, aggregation of small particles into larger ones, low velocity collisions, and charge accumulation are a few of the processes that influence particles in these systems. Examples of particles undergoing these processes include interstellar grains, protoplanetary particles, atmospheric aerosols, combustion products, and abiotic organic polymers. Although processes of the type described above span a wide range of disciplines, the study of these processes places common fundamental constraints on particle handling. Two common constraints are the need for long time periods during which the particles must be suspended and low relative velocities between particles. Experiments involving small particles generally require material be suspended for periods substantially longer than are practical in Earth\u27s 1 g gravitational field. However, one can investigate these processes with a general-purpose particle research facility (in particular, with the proposed Gas-Grain Simulation Facility) on the Space Station 1 \u273 . Because of the very low gravitational acceleration (microgravity) in the Earth orbital environment, many experiments deemed impractical or impossible to perform on Earth will become feasible. Such experiments are those in which gravity either interferes directly with the phenomenon under study (e.g., gravitational convection masks diffusional processes) or in which gravity precludes the establishment of the proper experimental conditions (e.g., in 1 g, gravity accelerates test objects to unacceptable velocities)

The mini-CIDEX GC/IMS: Analysis of cometary ice and dust

Comets are recognized as among the most scientifically important objects in the solar system. They are presumed relics of the early primitive material in the solar nebula and are believed to have provided a general enrichment of volatiles to the inner solar system. The Cometary Coma Chemical Composition (C4) Mission, a proposed Discovery-Class Mission, will analyze materials released into the coma, providing information leading to the understanding of the chemical composition and make-up of the cometary nucleus. As one of two scientific instruments in the C4 spacecraft, an advanced and streamlined version of the Cometary Ice and Dust Experiment (CIDEX), a mini-CIDEX, will employ an X-Ray Fluorescence (XRF) spectrometer to determine bulk elemental composition of cometary dust grains and a Gas Chromatograph/Ion Mobility Spectrometer (GC/IMS) for determination of the molecular composition of dust and ices following stepwise pyrolysis and combustion. A description of the mini-CIDEX IMS will be provided as well as data from analyses conducted using the mini-CIDEX breadboard instrument

A Solve-RD ClinVar-based reanalysis of 1522 index cases from ERN-ITHACA reveals common pitfalls and misinterpretations in exome sequencing

Purpose Within the Solve-RD project (https://solve-rd.eu/), the European Reference Network for Intellectual disability, TeleHealth, Autism and Congenital Anomalies aimed to investigate whether a reanalysis of exomes from unsolved cases based on ClinVar annotations could establish additional diagnoses. We present the results of the “ClinVar low-hanging fruit” reanalysis, reasons for the failure of previous analyses, and lessons learned. Methods Data from the first 3576 exomes (1522 probands and 2054 relatives) collected from European Reference Network for Intellectual disability, TeleHealth, Autism and Congenital Anomalies was reanalyzed by the Solve-RD consortium by evaluating for the presence of single-nucleotide variant, and small insertions and deletions already reported as (likely) pathogenic in ClinVar. Variants were filtered according to frequency, genotype, and mode of inheritance and reinterpreted. Results We identified causal variants in 59 cases (3.9%), 50 of them also raised by other approaches and 9 leading to new diagnoses, highlighting interpretation challenges: variants in genes not known to be involved in human disease at the time of the first analysis, misleading genotypes, or variants undetected by local pipelines (variants in off-target regions, low quality filters, low allelic balance, or high frequency). Conclusion The “ClinVar low-hanging fruit” analysis represents an effective, fast, and easy approach to recover causal variants from exome sequencing data, herewith contributing to the reduction of the diagnostic deadlock

Exobiology in Solar System Exploration

A symposium, 'Exobiology in Solar System Exploration,' was held on 24-26 Aug. 1988. The symposium provided an in-depth investigation of the role of Exobiology in solar system exploration. It is expected that the symposium will provide direction for future participation of the Exobiology community in solar system exploration and alert the Planetary community to the continued importance of an Exobiology Flight Program. Although the focus of the symposium was primarily on Exobiology in solar system exploration missions, several ground based and Earth-orbital projects such as the Search for Extraterrestrial Intelligence, Gas Grain Facility, and Cosmic Dust Collection Facility represent upcoming research opportunities planned to accommodate the goals and objectives of the Exobiology community as well. This report contains papers for all but one of the presentations given at the symposium

The Viking Biological Investigation: Preliminary Results

Three different types of biological experiments on samples of martian surface material ("soil") were conducted inside the Viking lander. In the carbon assimilation or pyrolytic release experiment, ^(14)CO_2 and ^(14)CO were exposed to soil in the presence of light. A small amount of gas was found to be converted into organic material. Heat treatment of a duplicate sample prevented such conversion. In the gas exchange experiment, soil was first humidified (exposed to water vapor) for 6 sols and then wet with a complex aqueous solution of metabolites. The gas above the soil was monitored by gas chromatography. A substantial amount of O_2 was detected in the first chromatogram taken 2.8 hours after humidification. Subsequent analyses revealed that significant increases in CO_2 and only small changes in N2 had also occurred. In the labeled release experiment, soil was moistened with a solution containing several ^(14)C-labeled organic compounds. A substantial evolution of radioactive gas was registered but did not occur with a duplicate heat-treated sample. Alternative chemical and biological interpretations are possible for these preliminary data. The experiments are still in process, and these results so far do not allow a decision regarding the existence of life on the planet Mars

Geolocator tagging links distributions in the non-breeding season to population genetic structure in a sentinel North Pacific seabird.

We tested the hypothesis that segregation in wintering areas is associated with population differentiation in a sentinel North Pacific seabird, the rhinoceros auklet (Cerorhinca monocerata). We collected tissue samples for genetic analyses on five breeding colonies in the western Pacific Ocean (Japan) and on 13 colonies in the eastern Pacific Ocean (California to Alaska), and deployed light-level geolocator tags on 12 eastern Pacific colonies to delineate wintering areas. Geolocator tags were deployed previously on one colony in Japan. There was strong genetic differentiation between populations in the eastern vs. western Pacific Ocean, likely due to two factors. First, glaciation over the North Pacific in the late Pleistocene might have forced a southward range shift that historically isolated the eastern and western populations. And second, deep-ocean habitat along the northern continental shelf appears to act as a barrier to movement; abundant on both sides of the North Pacific, the rhinoceros auklet is virtually absent as a breeder in the Aleutian Islands and Bering Sea, and no tagged birds crossed the North Pacific in the non-breeding season. While genetic differentiation was strongest between the eastern vs. western Pacific, there was also extensive differentiation within both regional groups. In pairwise comparisons among the eastern Pacific colonies, the standardized measure of genetic differentiation (FꞌST) was negatively correlated with the extent of spatial overlap in wintering areas. That result supports the hypothesis that segregation in the non-breeding season is linked to genetic structure. Philopatry and a neritic foraging habit probably also contribute to the structuring. Widely distributed, vulnerable to anthropogenic stressors, and exhibiting extensive genetic structure, the rhinoceros auklet is fully indicative of the scope of the conservation challenges posed by seabirds