Multicenter, prospective validation of a phenotypic algorithm to guide carbapenemase testing in carbapenem-resistant Pseudomonas aeruginosa using the ERACE-PA global surveillance program

Background: Carbapenemase-producing, carbapenem-resistant Methods: CRPA isolates were collected during the Enhancing Rational Antimicrobials for Results: A total of 807 CRPA were assessed, and 464 isolates met the algorithm criteria described above. Overall, testing was reduced by 43% compared with testing all CRPA. Carbapenemase-positive isolates missed by the algorithm were largely driven by Guiana extended spectrum (GES). Addition of the criterion of imipenem- or meropenem-resistant plus ceftolozane/tazobactam-nonsusceptible decreased the number of CP-CRPA missed by the algorithm (21 vs 40 isolates, respectively), reducing number of isolates tested by 39%. Conclusions: Application of the initial algorithm (imipenem- or meropenem-resistant plus ceftazidime-nonsusceptible plus cefepime-nonsusceptible) performed well in a global cohort, with 33% phenotypically carbapenemase-positive isolates. The addition of imipenem- or meropenem-resistant plus ceftolozane/tazobactam-nonsusceptible reduced the number of phenotypically carbapenemase-positive isolates missed and may be useful in areas with a prominence of GES

Evaluation of NG-Test Carba 5 for rapid phenotypic detection and differentiation of five common carbapenemase families: Results of a multicenter clinical evaluation

NG-Test Carba 5 is a rapi

A di-iron(III) mu-oxido complex as catalyst precursor in the oxidation of alkanes and alkenes

The oxido-bridged diiron(III) complex [Fe-2(mu-O)(mu-OAc)(DPEAMP)(2)](OCH3) (1), based on a new unsymmetrical ligand with an N4O donor set, viz. [2-((bis(pyridin-2-ylmethyl)amino)methyl)-6-((ethylamino)methyl)-4-meth-ylphenol (HDPEAMP)], has been prepared and characterized by spectroscopic methods and X-ray crystallog-raphy. The crystal structure of the complex reveals that each Fe(III) ion is coordinated by three nitrogen and three oxygen donors, two of which are the bridging oxido and acetate ligands. Employing H2O2 as a terminal oxidant, 1 is capable of oxidizing a number of alkanes and alkenes with high activity. The catalytic oxidation of 1,2-dimethylcyclohexane results in excellent retention of configuration. Monitoring of the reaction of 1 with H2O2 and acetic acid in the absence of substrate, using low-temperature UV-Vis spectroscopy, suggests the in situ formation of a transient Fe(III)(2)-peroxido species. While the selectivity and nature of oxidation products implicate a high-valent iron-oxido complex as a key intermediate, the low alcohol/ketone ratios suggest a simultaneous radical-based process.Peer reviewe

Trotter-Kato product formulae in Dixmier ideal

It is shown that for a certain class of the Kato functions the Trotter-Kato product formulae converge in Dixmier ideal C 1,$\infty$ in topology, which is defined by the $\times$ 1,$\infty$-norm. Moreover, the rate of convergence in this topology inherits the error-bound estimate for the corresponding operator-norm convergence. 1 since [24], [14]. Note that a subtle point of this program is the question about the rate of convergence in the corresponding topology. Since the limit of the Trotter-Kato product formula is a strongly continuous semigroup, for the von Neumann-Schatten ideals this topology is the trace-norm $\times$ 1 on the trace-class ideal C 1 (H). In this case the limit is a Gibbs semigroup [25]. For self-adjoint Gibbs semigroups the rate of convergence was estimated for the first time in [7] and [9]. The authors considered the case of the Gibbs-Schr{\"o}dinger semigroups. They scrutinised in these papers a dependence of the rate of convergence for the (exponential) Trotter formula on the smoothness of the potential in the Schr{\"o}dinger generator. The first abstract result in this direction was due to [19]. In this paper a general scheme of lifting the operator-norm rate convergence for the Trotter-Kato product formulae was proposed and advocated for estimation the rate of the trace-nor

Accuracy and Consistency of Radiographic Interpretation Among Clinical Instructors in Conjunction with a Training Program

There are inaccuracies and inconsistencies of radiographic interpretation among clinical instructors. The purpose of this investigation was to determine if a training program could improve the accuracy and consistency of instructors’ ratings of bone loss. A total of thirty-five clinical instructors consisting of periodontal faculty (periodontists and general dentists), dental hygiene faculty, and periodontal graduate students viewed projected digitized radiographic images and quantified bone loss for twenty-five teeth into four descriptive categories. Ratings of bone loss were made immediately before (pretest) and after (post-test 1) initiation of the training program and then again three months later (post-test 2). Ratings were compared to the correct choice categories as determined by direct measurement using the Schei ruler. Overall agreement with the correct choice improved over time (from 64.5 percent to 85.2 percent) with the greatest change from pretest (64.5 percent) to post-test 1 (76.5 percent). Mean and absolute differences improved in three of the four categories, but worsened in one from pretest to post-test 1. This category returned to its original high value at post-test 2. The greatest improvement in consistency among instructors’ ratings was seen in one of the four categories, which was “none” (no bone loss). Extension of the training program may further enhance the accuracy and consistency of instructors’ radiographic interpretation

Accuracy and Consistency of Radiographic Interpretation Among Clinical Instructors Using Two Viewing Systems

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/153540/1/jddj002203372006702tb04071x.pd