Landslide distribution and size in response to Quaternary fault activity: the Peloritani Range, NE Sicily, Italy

Landslides contribute to dismantle active mountain ranges and faults control the location of landslides. Yet, evidence of the long-term, regional dependency of landslides on active faults is limited. Previous studies focused on the transient effects of earthquakes on slope stability in compressive and transcurrent regimes. Here we show that in the Peloritani range, NE Sicily, Italy, one of the fastest uplifting areas in the Mediterranean, a clear geographical association exists between large bedrock landslides and active normal faults of the Messina Straits graben. By interpreting aerial photographs, we mapped 1590 landslides and sackungs and 626 fault elements and their facets in a 300 km2 area in the eastern part of the range. We used the new landslide and fault information, in combination with prior geological and seismic information, to investigate the association between bedrock landslides and faults. We find that the distribution and abundance of landslides is related to the presence of large active normal faults, and matches the pattern of the local historical seismicity. Landslide material is more abundant along the East Peloritani Fault System where the long-term activity of the faults, measured by the average yearly geological moment rate, is larger than in the West Peloritani Fault System where landslides are less abundant. Along the fault systems landslide material concentrates where the cumulated fault throws are largest. We conclude that large landslides and their cumulated volume are sensitive to local rates of tectonic deformation, and discriminate the deformation of the single fault segments that dissect the Peloritani range. Our findings are a direct test of landscape evolution models that predict higher rates of landslide activity near active faults. Our work opens up the possibility of exploiting accurate landslide and fault maps, in combination with geological and seismic information, to characterize the long-term seismic history of poorly instrumented active regions. Copyright © 2015 The Authors Earth Surface Processes and Landforms Published by John Wiley & Sons Lt

Innovative Fermented Beverages Made with Red Rice, Barley, and Buckwheat

The increase in food intolerances, allergies, and food-based lifestyle choices has dramatically increased the consumer demand for healthy foods characterized by pleasant sensory traits. In such a context, innovative cereal-based beverages are characterized by high nutritional value, pleasant palatability, and potential healthy properties. In the present study, a pool of 23 lactic acid bacteria strains was preliminary assayed as monocultures for the fermentation of three ad hoc formulated cereal- (red rice and barley) and pseudocereal (buckwheat) -based substrates. Eight strains with the best performance in terms of acidification rate were selected for the formulation of three multiple strain cultures to be further exploited for the manufacture of laboratory-scale prototypes of fermented beverages. The compositional and microbiological features of the three experimental beverages highlighted their high biological value for further exploitation

Fattori influenti sul ricorso ad Internet nei processi gestionali delle micro-imprese

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Exploitation of Tenebrio molitor larvae as biological factories for human probiotics, an exploratory study

The exploitation of yellow mealworm (Tenebrio molitor) larvae for the bioaugmentation of probiotic Bacillus clausii strains was evaluated during a 7-day rearing period. qPCR was applied to evaluate the persistence and growth of B. clausii in the rearing substrate and larvae (washed and non-washed). Moreover, the effect of freeze-drying of larvae on B. clausii viability was evaluated. The results demonstrated the suitability of yellow mealworm as biological factories for the multiplication of B. clausii through a simple and inexpensive procedure, in view of the further application of larvae as foods and food ingredients. In more detail, an increase in the load of B. clausii was observed during the 7-day rearing of larvae fed wheat middlings spiked with 1 Log cells g−1. Further research is needed to evaluate the most suitable technologies and processing parameters for obtaining yellow mealworm-based ingredients with a stable and active population of probiotic B. clausii

Acquiring vulnerability indicators to geo-hydrological hazards: An example of mobile phone-based data collection

Abstract Geo-hydrological risk reduction is a key issue for local governments in Italy. In this context, a collaboration was undertaken between multiple actors in the La Spezia municipality aimed at: (i) monitoring building characteristics, using specific and valuable indicators, and (ii) increasing the knowledge of geo-hydrological hazards across residents and local land planners (iii) implementing local emergency civil protection plan. An extensive mobile data collection was carried out through apps specifically developed for Android and IOS mobile devices. The digital forms were differentiated on the basis of the potential hazard: one of 46 fields and one of 125 fields designed for buildings respectively located in flood prone areas and in medium to very high landslide susceptibility areas. The digital version of the forms was designed using the Open Data Kit (ODK) and GISCloud client-server approach. All the collected data, including geospatial locations and images, were automatically sent to a central server, stored and organized in a database. Geospatial data-analysis and maps resulted useful in evaluating possible impacts to exposed buildings to potential geo-hydrological processes. The proposed public participation method for data-gathering increased the knowledge across residents providing a better understanding of the urban systems, of the buildings condition and their relation respect to the geo-hydrological risk. The method can be considered as part of the decision support systems for civil protection purposes to better planning geo-hydrological mitigation measures. The application of mobile technology for data collection can be effectively used when local government resources are limited

Study of the bacterial diversity of foods: PCR-DGGE versus LH-PCR

The present study compared two culture-independent methods, polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) and length-heterogeneity polymerase chain reaction (LH-PCR), for their ability to reveal food bacterial microbiota. Total microbial DNA and RNA were extracted directly from fourteen fermented and unfermented foods, and domain A of the variable regions V1 and V2 of the 16S rRNA gene was analyzed through LH-PCR and PCR-DGGE. Finally, the outline of these analyses was compared with bacterial viable counts obtained after bacterial growth on suitable selective media. For the majority of the samples, RNA-based PCR-DGGE revealed species that the DNA-based PCR-DGGE was not able to highlight. When analyzing either DNA or RNA, LH-PCR identified several lactic acid bacteria (LAB) and coagulase negative cocci (CCN) species that were not identified by PCR-DGGE. This phenomenon was particularly evident in food samples with viable loads b 5.0 Log cfu g−1 . Furthermore, LH-PCR was able to detect a higher number of peaks in the analyzed food matrices relative to species identified by PCR-DGGE. In light of these findings, it may be suggested that LH-PCR shows greater sensitivity than PCR-DGGE. However, PCR-DGGE detected some other species (LAB included) that were not detected by LH-PCR. Therefore, certain LH-PCR peaks not attributed to known species within the LH-PCR database could be solved by comparing them with species identified by PCR-DGGE. Overall, this study also showed that LH-PCR is a promising method for use in the food microbiology field, indicating the necessity to expand the LH-PCR database, which is based, up to now, mainly on LAB isolates from dairy produc

The Microbial Diversity of Non-Korean Kimchi as Revealed by Viable Counting and Metataxonomic Sequencing

Kimchi is recognized worldwide as the flagship food of Korea. To date, most of the currently available microbiological studies on kimchi deal with Korean manufactures. Moreover, there is a lack of knowledge on the occurrence of eumycetes in kimchi. Given these premises, the present study was aimed at investigating the bacterial and fungal dynamics occurring during the natural fermentation of an artisan non-Korean kimchi manufacture. Lactic acid bacteria were dominant, while Enterobacteriaceae, Pseudomonadaceae, and yeasts progressively decreased during fermentation. Erwinia spp., Pseudomonas veronii, Pseudomonas viridiflava, Rahnella aquatilis, and Sphingomonas spp. were detected during the first 15 days of fermentation, whereas the last fermentation phase was dominated by Leuconostoc kimchi, together withWeissella soli. For the mycobiota at the beginning of the fermentation process, Rhizoplaca and Pichia orientalis were the dominant Operational Taxonomic Units (OTUs) in batch 1, whereas in batch 2 Protomyces inundatus prevailed. In the last stage of fermentation, Saccharomyces cerevisiae, Candida sake, Penicillium, and Malassezia were the most abundant taxa in both analyzed batches. The knowledge gained in the present study represents a step forward in the description of the microbial dynamics of kimchi produced outside the region of origin using local ingredients. It will also serve as a starting point for further isolation of kimchi-adapted microorganisms to be assayed as potential starters for the manufacturing of novel vegetable preserves with high quality and functional traits

Skin dysbiosis and Cutibacterium acnes biofilm in inflammatory acne lesions of adolescents

Acne vulgaris is a common inflammatory disorder affecting more than 80% of young adolescents. Cutibacterium acnes plays a role in the pathogenesis of acne lesions, although the mechanisms are poorly understood. The study aimed to explore the microbiome at different skin sites in adolescent acne and the role of biofilm production in promoting the growth and persistence of C. acnes isolates. Microbiota analysis showed a significantly lower alpha diversity in inflammatory lesions (LA) than in non-inflammatory (NI) lesions of acne patients and healthy subjects (HS). Differences at the species level were driven by the overabundance of C. acnes on LA than NI and HS. The phylotype IA1 was more represented in the skin of acne patients than in HS. Genes involved in lipids transport and metabolism, as well as potential virulence factors associated with host-tissue colonization, were detected in all IA1 strains independently from the site of isolation. Additionally, the IA1 isolates were more efficient in early adhesion and biomass production than other phylotypes showing a significant increase in antibiotic tolerance. Overall, our data indicate that the site-specific dysbiosis in LA and colonization by virulent and highly tolerant C. acnes phylotypes may contribute to acne development in a part of the population, despite the universal carriage of the microorganism. Moreover, new antimicrobial agents, specifically targeting biofilm-forming C. acnes, may represent potential treatments to modulate the skin microbiota in acne

Dactinomycin induces complete remission associated with nucleolar stress response in relapsed/refractory NPM1-mutated AML

Acute myeloid leukemia (AML) with mutated NPM1 accounts for one-third of newly diagnosed AML. Despite recent advances, treatment of relapsed/refractory NPM1-mutated AML remains challenging, with the majority of patients eventually dying due to disease progression. Moreover, the prognosis is particularly poor in elderly and unfit patients, mainly because they cannot receive intensive treatment. Therefore, alternative treatment strategies are needed. Dactinomycin is a low-cost chemotherapeutic agent, which has been anecdotally reported to induce remission in NPM1-mutated patients, although its mechanism of action remains unclear. Here, we describe the results of a single-center phase 2 pilot study investigating the safety and efficacy of single-agent dactinomycin in relapsed/refractory NPM1-mutated adult AML patients, demonstrating that this drug can induce complete responses and is relatively well tolerated. We also provide evidence that the activity of dactinomycin associates with nucleolar stress both in vitro and in vivo in patients. Finally, we show that low-dose dactinomycin generates more efficient stress response in cells expressing NPM1 mutant compared to wild-type cells, suggesting that NPM1-mutated AML may be more sensitive to nucleolar stress. In conclusion, we establish that dactinomycin is a potential therapeutic alternative in relapsed/refractory NPM1-mutated AML that deserves further investigation in larger clinical studies

Distribution of Transferable Antibiotic Resistance Genes in Laboratory-Reared Edible Mealworms (Tenebrio molitor L.)

In the present study, the distribution of antibiotic resistance genes in laboratory-reared fresh mealworm larvae (Tenebrio molitor L.), their feeding substrates (carrots and wheatmeal), and frass was assessed. Microbial counts on selective media added with antibiotics highlighted the presence of lactic acid bacteria resistant to ampicillin and vancomycin and, more specifically, enterococci resistant to the latter antibiotic. Moreover, staphylococci resistant to gentamicin, erythromycin, tetracycline, and vancomycin were detected. Enterobacteriaceae resistant to ampicillin and gentamicin were also found, together with Pseudomonadaceae resistant to gentamicin. Some of the genes coding for resistance to macrolide-lincosamide-streptogramin B (MLSB) [erm(A), erm(C)], vancomycin [vanA, vanB], tetracycline [tet(O)], and β-lactams [mecA and blaZ] were absent in all of the samples. For the feeding substrates, organic wheatmeal was positive for tet(S) and tet(K), whereas no AR genes were detected in organic carrots. The genes tet(M), tet(K), and tet(S) were detected in both mealworms and frass, whereas gene aac-aph, coding for resistance to amynoglicosides was exclusively detected in frass. No residues for any of the 64 antibiotics belonging to 10 different drug classes were found in either the organic wheatmeal or carrots. Based on the overall results, the contribution of feed to the occurrence of antibiotic resistance (AR) genes and/or antibiotic-resistant microorganisms in mealworm larvae was hypothesized together with vertical transmission via insect egg smearing