'Drivin' with your eyes closed': Results from an international, blinded simulation experiment to evaluate spatial stock assessments

Spatial models enable understanding potential redistribution of marine resources associated with ecosystem drivers and climate change. Stock assessment platforms can incorporate spatial processes, but have not been widely implemented or simulation tested. To address this research gap, an international simulation experiment was organized. The study design was blinded to replicate uncertainty similar to a real-world stock assessment process, and a data-conditioned, high-resolution operating model (OM) was used to emulate the spatial dynamics and data for Indian Ocean yellowfin tuna (Thunnus albacares). Six analyst groups developed both single-region and spatial stock assessment models using an assessment platform of their choice, and then applied each model to the simulated data. Results indicated that across all spatial structures and platforms, assessments were able to adequately recreate the population trends from the OM. Additionally, spatial models were able to estimate regional population trends that generally reflected the true dynamics from the OM, particularly for the regions with higher biomass and fishing pressure. However, a consistent population biomass scaling pattern emerged, where spatial models estimated higher population scale than single-region models within a given assessment platform. Balancing parsimony and complexity trade-offs were difficult, but adequate complexity in spatial parametrizations (e.g., allowing time- and age-variation in movement and appropriate tag mixing periods) was critical to model performance. We recommend expanded use of high-resolution OMs and blinded studies, given their ability to portray realistic performance of assessment models. Moreover, increased support for international simulation experiments is warranted to facilitate dissemination of methodology across organizations

Comparisons of landings to scientific advice indicate overshooting within the common TAC for skates and rays in the Northeast Atlantic

The International Council for the Exploration of the Sea (ICES) typically provides advice on fishing opportunities on a stock-by-stock basis. Nevertheless, levels of total allowable catch (TAC) are sometimes set for a collection of stocks and species (i.e. a common TAC). An explicit expectation of these is that landings will scale with ICES advice, especially when ICES advice is used to calculate the common TAC. This expectation is tested for skates and rays in the Northeast Atlantic, spanning 26 stocks, 8 species, and 3 ecoregions. Using ICES landings and ICES advice data from 2016 to 2022, we show that landings of several stocks and species have overshot their respective ICES advice, whereas others have undershot. Specifically, some stocks of blonde ray (Raja brachyura) in North Sea and Celtic Seas ecoregions are being landed at a rate that often exceeds double its ICES advice. By collating species based on their ICES assessment category and life-history traits, we find that those considered data-poor and potentially most vulnerable to fishing are consistently landed at higher-than-expected rates in the Celtic Seas. This study questions the appropriateness of a common TAC for skates and rays and calls for shifts towards the use of single-stock catch allocations and the application of advanced stock assessment methodologies

Stopover dynamics of 12 passerine migrant species in a small Mediterranean island during spring migration

Small coastal islands offer landing opportunities for large numbers of migratory birds following long sea crossings. However, they do not always provide sufficient refueling opportunities, thus raising questions about their importance for the success of migratory journeys. Here we analyzed a large dataset collected during 3 years of captures and recaptures of 12 species on the island of Ponza, central Italy, to determine the importance of the island for refueling. Despite the very large amount of birds on the island, only a very small fraction (usually below 2%) stayed on the island for longer than 1 day. These birds had low energy stores and, in most cases, they were not able to successfully refuel on Ponza. Only two species (Subalpine Warbler and Common Chiffchaff) had a positive fuel deposition rate, possibly as a result of the better suitability of the island's habitat to these two species. We underline that the large use of the island despite the relatively low refueling opportunities may be due to other aspects that it may offer to the birds. Possibly, birds just landed after a long sea crossing may require a short rest or sleep and can find opportunities to do that on the islands, reinitiating their onward flight after just a few hours. Understanding the role of these islands for migratory birds will be important for conservation, since setting priorities for protection might be misled if considering only refueling success as an important variable

Diversity and Structure of the Endophytic Bacterial Communities Associated With Three Terrestrial Orchid Species as Revealed by 16S rRNA Gene Metabarcoding

none4siThe endophytic microbiota can establish mutualistic or commensalistic interactions within the host plant tissues. We investigated the bacterial endophytic microbiota in three species of Mediterranean orchids (Neottia ovata, Serapias vomeracea, and Spiranthes spiralis) by metabarcoding of the 16S rRNA gene. We examined whether the different orchid species and organs, both underground and aboveground, influenced the endophytic bacterial communities. A total of 1,930 operational taxonomic units (OTUs) were obtained, mainly Proteobacteria and Actinobacteria, whose distribution model indicated that the plant organ was the main determinant of the bacterial community structure. The co-occurrence network was not modular, suggesting a relative homogeneity of the microbiota between both plant species and organs. Moreover, the decrease in species richness and diversity in the aerial vegetative organs may indicate a filtering effect by the host plant. We identified four hub OTUs, three of them already reported as plant-associated taxa (Pseudoxanthomonas, Rhizobium, and Mitsuaria), whereas Thermus was an unusual member of the plant microbiota. Core microbiota analysis revealed a selective and systemic ascent of bacterial communities from the vegetative to the reproductive organs. The core microbiota was also maintained in the S. spiralis seeds, suggesting a potential vertical transfer of the microbiota. Surprisingly, some S. spiralis seed samples displayed a very rich endophytic microbiota, with a large number of OTUs shared with the roots, a situation that may lead to a putative restoring process of the root-associated microbiota in the progeny. Our results indicate that the bacterial community has adapted to colonize the orchid organs selectively and systemically, suggesting an active involvement in the orchid holobiont.openAlibrandi, Pasquale; Schnell, Sylvia; Perotto, Silvia; Cardinale, MassimilianoAlibrandi, Pasquale; Schnell, Sylvia; Perotto, Silvia; Cardinale, Massimilian

Heat loss in sleeping Garden Warblers (Sylvia borin) during migration

For small songbirds, energy is often a limiting factor during migration and, for this reason, they are forced to alternate nocturnal flights with stopovers to rest and replenish energy stores. Stopover duration has a key role for a successful migration and may have an important impact on fitness. Thus, migrants need to optimize their energy consumption at this stage to reduce their permanence at the site. A recent study has shown that lean individuals reduce their metabolic rate when tucking the head in the feathers during sleep. The underlying mechanism is very likely a reduction in conductance, but the thermoregulatory benefit of the increased insulation has never been quantified yet. Here, we compared heat loss in individual migratory birds while sleeping in different postures. Using a thermal camera and a within-individual approach, we estimated that Garden Warblers can reduce their rate of heat loss by 54% by sleeping with the head tucked in the feathers. This energy saving has a relevant impact on the individual’s energy balance because it can account for up to 8.69% of daily energy expenditure during stopover. Our study provides novel and important information to understand the fundamental role of thermoregulatory strategies on bird’s energy management

Scientific, Technical and Economic Committee for Fisheries (STECF) Report of the SGMED-09-02 Working Group on the Mediterranean - Part I

SGMED-09-02 was held on 8-12 June 2009 in Sardinia (Italy). The report is a compilation of information on existing fisheries and stock data in order to update the status of the main demersal and small pelagic stocks. The report deals with assessment of historic and recent trends in stock parameters (stock size, recruitment and exploitation) and relevant scientific advice. STECF reviewed the report during its Plenary meeting on 9-13 November 2009.JRC.DG.G.4-Maritime affair

Development of control strategies for bacteria and fungi associated with a micropropagated new cultivar of orange-fleshed sweet potato (Ipomoea batatas cv. Agrosavia–Aurora)

Vegetative propagation of sweet potato lead to the accumulation of diseases from generation to generation, which represents a threat to both productivity and conservation of genetic resources. In vitro techniques can help to overcome phytosanitary problems by applying plant material cleaning strategies. The objective of this study was to develop in vitro micropropagation strategies for the production of high-quality plant material of an orange-fleshed variety of sweet potato recently released in Colombia. Molecular identification of contaminating microorganisms was performed by sequencing the 16S rRNA gene for bacteria and ITS for fungi. Five disinfection protocols were evaluated, three of which were previously developed for sweet potato and included disinfection with 0.5, 1, and 2% sodium hypochlorite respectively, while two protocols are proposed in this work and included washing with povidone-iodine, disinfection with sodium hypochlorite 2%; one of these two new protocols also contains acetic acid and quaternary ammonium. For the evaluation of the viability of in vitro plants after disinfection, they were acclimatized in a greenhouse, reintroduced, and a molecular testing by PCR of 16S rRNA gene and ITS was carried out to verify the phytosanitary status of the material. The contaminating microorganisms found were filamentous fungi of the genera Fusarium, Sarocladium, Cladosporium and Aspergillus, yeasts of the genera Pseudozyma and Moesziomyces, and the actinobacterium Curtobacterium sp. The results indicated that washing with povidone-iodine and disinfection with 2% sodium hypochlorite, acetic acid and quaternary ammonium was the most efficient disinfection protocol, reducing the number of contaminated cultures by up to 10% and eradicating 70% of contaminants. The in vitro plants established in the greenhouse remained healthy and, after reintroduction, the molecular test for bacteria and fungi was negative. These results allowed the generation of an optimized protocol that can be incorporated into the in vitro micropropagation process to generate contamination-free sweet potato seeds

Scientific, Technical and Economic Committee for Fisheries (STECF) - Report of the SGMED-09-03 Working Group on the Mediterranean Part II

SGMED-09-03 meeting was held on 14-18 December 2009 in Barza d¿ Ispra (Italy). The report provides specific stock (by species and GSAs) predictions of stock size, fishing mortality and catches in short term (2009-2011) and medium term (2009-2018) under different management scenarios, for the main demersal and small pelagic Mediterranean stocks. STECF reviewed the report during its Plenary meeting on 26-30 April 2010 in Norwich.JRC.DG.G.4-Maritime affair