Grasses (Poaceae) of the District of Coronel Rosales (Province of Buenos Aires, Argentina)

Las gramíneas (Poáceas) conforman una de las familias más numerosas, con más de 670 géneros y cerca de 11.000 especies descriptas. Se (1) efectuó un inventario de las gramíneas del Partido de Coronel Rosales, Buenos Aires, Argentina, y (2) ofreció una síntesis considerando sus usos etnobotánicos y características ecológicas. Durante 2006, 2007 y 2008 el material recolectado se identificó mediante el uso de claves taxonómicas. La familia Poaceae representó el 25% de las especies del Partido. Se confirmó la presencia de 95 especies de Poaceae distribuidas en 17 tribus y 47 géneros. De éstas, las especies autóctonas constituyeron cerca del 45% de la flora espontánea. El 36 ó 26% de las especies halladas fueron gramíneas perennes de estación cálida o fría, respectivamente. El porcentaje restante lo constituyeron las especies anuales. Un 46,3% y un 53,7% de las especies de gramíneas estudiadas tuvieron la senda fotosintética C3 y C4, respectivamente. Se destacaron varios usos: (1) producción de forraje para el ganado doméstico, (2) medicinales, (3) fijadoras de médanos y de terrenos con suelos sueltos (es decir de textura gruesa), (4) ornamentales, (5) utilización como césped, (6) alimenticios, y (7) varios usos industriales: fabricación de conglomerados, pasta de papel y canastas.Grasses (i.e., Poaceae) are one of the more numerous, described families with more than 670 genera and about 11,000 species. This work includes (1) a plant record of the grasses of the District of Coronel Rosales, Buenos Aires, Argentina, and (2) a synthesis of their ethnobotanical uses and ecological characteristics. During 2006, 2007 and 2008, we identified the sampled material using taxonomical keys. Twenty five percent of the species of the District was represented by the Poaceae family. Ninety five Poaceae species were distributed in 17 tribes and 47 genera. Native species were approximately 45% of the wild flora. Warm- or cool-season perennial grasses were 36 or 26%, respectively, of the found species; annual species constituted the remaining percentage. A 46.3% or 53.7% of the study grass species showed the C3 or C4, photosynthetic pathway, respectively. The study species can be used as: (1) forage for domestic livestock, (2) medicinal plants, (3) dune and sandy, loose land fixers, (4) ornamentals, (5) lawn, (6) food, and (7) a source for building woody, housing materials; paper pasta and canastas.Fil: Cardillo, Daniela Solange. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); ArgentinaFil: Busso, Carlos Alberto. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); ArgentinaFil: Ambrosino, Mariela Lis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Torres, Yanina Alejandra. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ithurrart, Leticia Soledad. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); ArgentinaFil: Montenegro, Oscar Alberto. Provincia de Buenos Aires. Ministerio de Asuntos Agrarios. Chacra Experimental de Patagones; Argentin

Plant species identity and richness influence microbial respiration of soil microorganisms on various functional groups in northeastern Patagonia, Argentina

Studies on basal soil respiration (i.e., under undisturbed conditions) are very important because they can be used as indirect indicators of the biological activity in those soils; this ecological process is recognized as the major source of carbon flux from the soil surface, and one of the crucial components of the carbon cycle in terrestrial ecosystems. The objectives of this study were to determine the microbial respiration of soil microorganisms at various levels of plant species richness and developmental morphology stages in various perennial grass (Nassella longiglumis, N. tenuis, Amelichloa ambigua), and herbaceous (Atriplex semibaccata) and woody (Larrea divaricata, Schinus fasciculatus) dicots grown in experimental plots during 2013 and 2014. There were 54 experimental plots. On each of 6 blocks, there was a plot (1.25x1.25m) for each of the 6 species (monocultures) and one plot each having combinations of 2, 4 or 6 species. Six hundred and twenty nine plants were reserved to replace dead plants in the plots [629+1944 plants from the plots (54 plots x 36 plants per plot)=2573 plants in total]. An auger (3 cm diameter, 20 cm length), was used to obtain six replicate root + soil samples at each of four sampling times during those years. Basal soil respiration was similar (p>0.05) or greater (p<0.05), but ever lower, as plant species richness increased. Our results demonstrated that the plant species differences in microbial respiration in the experimental plots were species richness-, developmental morphology stage-, and sampling-time dependents.Fil: Cardillo, Daniela Solange. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Busso, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Ambrosino, Mariela Lis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Facultad de Ciencias Exactas y Naturales Unlpamp;Fil: Torres, Yanina Alejandra. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Ithurrart, Leticia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Palomo, Iris Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentin

Species identity, richness and developmental stage of morphology affect enzymatic activiy of the soil microorganisms in arid Patagonia, Argentina

Microbial communities are a key for terrestrial ecosystem functioning. However, their responses to changes in plant species identity and richness, and stages of developmental morphology have been rarely investigated. The objective of this study was to evaluate the impact of the identity, richness and stage of developmental morphology of plant species on soil microbial communities throughout the enzymatic activity of the dehydrogenase. Studies were conducted under wild, field conditions, and on field experimental plots having different species richness. Treatments included a control (intraspecific monocultures) or combinations of 2, 4, or 6 species pertaining to different functional groups (i.e., perennial either grasses or herbaceous dicots or shrubs). The grass Nassella tenuis and the shrub Larrea divaricata showed a lower (p<0.05) activity of the dehydrogenase than most of the other studied species under wild, field conditions. The enzymatic activity of the dehydrogenase was either similar or greater (p<0.05; e.g., Amelichloa ambigua, L. divaricata), but not lower, as species richness increased in the reproductive stage of developmental morphology. Finally, the dehydrogenase activity was in general greater (p<0.05) at the reproductive (late spring) than vegetative (late fall) stage of developmental morphology in all studied species. These results indicate that species identity, richness and stage of developmental morphology might be important determinants of the degree of microbial activity in the soil.Fil: Cardillo, Daniela Solange. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Busso, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Ambrosino, Mariela Lis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Torres, Yanina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Ithurrart, Leticia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Palomo, Iris Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentin

Plant Species Richness and Developmental Morphology Stage Influence Mycorrhizal Patagonia Plants Root Colonization

The objectives of this study were to determine the percentage of root colonization by arbuscularmycorrhizal (AM) fungi at various levels of plant species richness and developmental morphology stages in various perennial grass, and herbaceous and woody dicots species using experimental plots during 2013 and 2014. An auger was used to obtain six replicate root + soil samples at each sampling time on each of the study parameters. Roots were washed free of soil, and percentage AM was determined. The shrub Larrea divaricate was the species which showed the lowest percentage of colonization by AM at the vegetative developmental morphology stage at the monocultures and six-species-mixtures on the experimental plots. Dicots, but not grass, species showed a greater percentage colonization by AM fungi at the greatest (i.e., six-species-mixtures) than lowest (i.e., monocultures) species richness. Although at different degrees of species richness and developmental morphology stages, the perennial grasses Nassella longiglumis and N. tenuis, the herbaceous dicot Atriplex semibaccata, and the shrubs L. divaricata and Schinus fasciculatus showed a greater (p < 0.050) percentage colonization by AM fungi during the second than the first study year. Even though it was speciesand sampling time-dependent, percentage colonization by AM fungi increased as species richness also increased most of the times. Our results demonstrated that the plant species diff erences in percentage colonization by AM fungi in the experimental plots were species richness-, developmental morphology stage-, and sampling-time dependents.Fil: Cardillo, Daniela Solange. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; ArgentinaFil: Busso, Carlos Alberto. Universidad Nacional del Sur. Departamento de Agronomia. Laboratorio de Ecología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Ambrosino, Mariela Lis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional de La Pampa; ArgentinaFil: Ithurrart, Leticia Soledad. Universidad Nacional del Sur. Departamento de Agronomia. Laboratorio de Ecología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; ArgentinaFil: Torres, Yanina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Agronomia. Laboratorio de Ecología; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Palomo, Iris Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentin

Plant Species and Defoliation Efects on Soil Nitrogen Mineralization in a Semiarid Rangeland of Argentina

The objective of this study was to evaluate the efects of moderate defoliation and grass species on soil nitrogen (N) mineralization in a semiarid grassland of northeastern Patagonia, Argentina. Studied species were Poa ligularis and Nassella tenuis (desirable/preferred by cattle) and Amelichloa ambigua (undesirable/non preferred). Two defoliations were made to a 5 cm stubble height during the growing season. Hypotheses were that (1) net N mineralization and N availability in the soil are increased by a moderate defoliation and greater species forage quality, and (2) potential N mineralization is higher in the soil beneath the desirable than undesirable species. In 2013 and 2014, in situ net N mineralization was estimated using the tube incubation technique under feld conditions. Potential N mineralization was estimated by long-term laboratory incubations. Defoliation treatments did not afect the soil inorganic N dynamics. The soil under A. ambigua showed a greater in situ net N mineralization than other species, but only in 2013. Poa ligularis presented the highest initial inorganic N and potentially mineralizable N values. However, the opposite was recorded in this specie for the mineralization constant rate. These results demonstrated that moderate defoliations did not afect soil N availability, and presence of the desirable perennial grasses increased the potential N mineralization pool. Sustainable management practices that promote the persistence of these species in plant community are important to maintain soil fertility on semiarid grasslands

Total and structure colonization by arbuscular mycorrhizal fungi in native, perennial grasses of different forage quality exposed to defoliation

Defoliation can compromise the quality and quantity of colonization by arbuscular mycorrhizal fungi (AMF), which contribute to vegetation persistence in semiarid rangelands. The effects of defoliation on total arbuscular mycorrhizal (AM) colonization percentage and on that of its structures (i.e., vesicles, arbuscules) were evaluated on three native, rangeland perennial grass species. These species (i.e., Poa ligularis, Nassella tenuis and Amelichloa ambigua) show different palatability to domestic livestock in Central Argentina. In August 2012, soil + roots (0–10 cm depth) were sampled below the foliage of 12 plants of each species. Half of the plants were then defoliated to 5 cm stubble leaving active meristems intact after defoliation. The other half remained undefoliated. In September, immediately after the differentiation of apical meristems from vegetative to reproductive, soil + roots samples were again obtained and thereafter plants were once again defoliated. The final soil + root sampling was conducted in October (6 plants/species/treatment). The study was repeated on a different plant set during 2013. The percentage of total AM colonization and that its structures were determined. Palatable species did not reach a greater total colonization by AMF in their roots than A. ambigua. Treatments affected the total colonization only at some sampling times (e.g., when it did affect at N. tenuis, the effect of defoliation was not consistent during the study years). At the last date, A. ambigua showed a greater percentage of arbuscules in both defoliation treatments in 2012 and on defoliated plants in 2013. In general, P. ligularis showed a greater vesicle percentage than the other species. Management practices which allow the recuperation of the perennial grasses after a moderate grazing, will not affect considerably their symbiotic relationships.Fil: Ambrosino, Mariela Lis. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Busso, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Cabello, Marta Noemí. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; ArgentinaFil: Velázquez, María Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; ArgentinaFil: Torres, Yanina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Ithurrart, Leticia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Cardillo, Daniela Solange. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Palomo, Iris Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentin

Plant litter decomposition in a semi-arid rangeland of Argentina: species and defoliation effects

Plant litter decomposition is critical for terrestrial ecosystem productivity. Poa ligularis Nees ex Steud and Nassella tenuis (Phil.) Barkworth are native, desirable perennial grasses in central Argentina’s rangelands. Amelichloa ambigua (Speg.) Arriaga & Barkworth is only consumed when a better forage is unavailable. Litter traps were used to collect aboveground litter during two years. In March 2012, six bags, each one containing either leaf blade (three bags, one per species) or root litter (three bags, one per species) of the three species were located below the canopy of each replicate plant of the studied species (hereafter referred to as ‘location’). Blade litter bags were located on the soil surface, and root litter bags buried at 10 cm soil depth. This allowed evaluation of the effects of defoliation, the different species canopies and the microbial community activity around their roots on decomposition of above- and belowground litter. For each species, twenty plants were either defoliated twice (5 cm stubble height) or remained undefoliated during the growing season. Litter bags were collected after 2, 7, 13 and 24 months incubation. The study was repeated in 2013, with additional bags were placed for N content determination on leaf blade and root litters. Aboveground litter production was highest in P. ligularis; however, no differences were observed among species when the effect of plant size was eliminated. Aboveground litter of desirable species had higher N content and decomposed faster than that of A. ambigua. The opposite was recorded for root litter. Defoliation had no effect on litter decomposition, but location effects were detected after one year of incubation. Desirable perennial grasses promoted organic matter loss from litter, a key factor in increasing soil fertility in this semiarid ecosystem.Fil: Ambrosino, Mariela Lis. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Busso, Carlos Alberto. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Torres, Yanina Alejandra. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ithurrart, Leticia Soledad. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Martinez, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Minoldo, Gabriela Verónica. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Cardillo, Daniela Solange. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Palomo, Iris Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentin

Communities of arbuscular mycorrhizal fungi associated with perennial grasses of different forage quality exposed to defoliation

Vegetation management practices, such as defoliation may alter the composition of plant communities and/orthe fungi-forming arbuscular mycorrhiza (AMF). We determined the species identity, density, frequency anddiversity of AMF spores from soil under the canopies of three native perennial grass species in rangelands ofArgentina: 1) Poa ligularis and Nassella tenuis (preferred by livestock) and 2) Amelichloa ambigua (not preferred).For each species, plants either remained undefoliated or were defoliated twice to a 5 cm stubble height duringthe growing season. Most active meristems remained on the plants after defoliations. AMF communities weresampled prior to (i.e., 48 soil samples) and following (i.e., 72 soil samples) each defoliation event. Spores weregrouped in 15 morphospecies. Density, richness and diversity of AMF spores were not influenced by defoliation,and species richness and diversity of AMF were similar among species. Total spore density was greatest for P.ligularis at the sampling prior to defoliation, but this difference did not persist following the defoliation events.The most abundant AMF families were: Acaulosporaceae, Diversisporaceae and Glomeraceae. These resultsdemonstrate that responses of the studied variables were insensitive to the defoliation treatments, and werelargely unaffected by the studied grass species.Fil: Ambrosino, Mariela Lis. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cabello, Marta Noemí. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Busso, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Velázquez, María Silvana. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Torres, Yanina Alejandra. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Cardillo, Daniela Solange. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Ithurrart, Leticia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Montenegro, Oscar Alberto. Provincia de Buenos Aires. Ministerio de Asuntos Agrarios. Chacra Experimental de Patagones; ArgentinaFil: Giorgetti, Hugo Dosindo. Provincia de Buenos Aires. Ministerio de Asuntos Agrarios. Chacra Experimental de Patagones; ArgentinaFil: Rodriguez, Gustavo. Provincia de Buenos Aires. Ministerio de Asuntos Agrarios. Chacra Experimental de Patagones; Argentin