New results from an extensive aging test on bakelite Resistive Plate Chambers

We present recent results of an extensive aging test, performed at the CERN Gamma Irradiation Facility on two single--gap RPC prototypes, developed for the LHCb Muon System. With a method based on a model describing the behaviour of an RPC under high particle flux conditions, we have periodically measured the electrode resistance R of the two RPC prototypes over three years: we observe a large spontaneous increase of R with time, from the initial value of about 2 MOhm to more than 250 MOhm. A corresponding degradation of the RPC rate capabilities, from more than 3 kHz/cm2 to less than 0.15 kHz/cm2 is also found.Comment: 6 pages, 7 figures, presented at Siena 2002, 8th Topical Seminar on Innovative Particle and Radiation Detectors 21-24 October 2002, Siena, Ital

Extracellular vesicle-induced differentiation of neural stem progenitor cells

Neural stem progenitor cells (NSPCs) from E13.5 mouse embryos can be maintained in culture under proliferating conditions. Upon growth-factor removal, they may differentiate toward either neuronal or glial phenotypes or both. Exosomes are small extracellular vesicles that are part of the cell secretome; they may contain and deliver both proteins and genetic material and thus play a role in cell–cell communication, guide axonal growth, modulate synaptic activity and regulate peripheral nerve regeneration. In this work, we were interested in determining whether NSPCs and their progeny can produce and secrete extracellular vesicles (EVs) and if their content can affect cell differentiation. Our results indicate that cultured NSPCs produce and secrete EVs both under proliferating conditions and after differentiation. Treatment of proliferating NSPCs with EVs derived from differentiated NSPCs triggers cell differentiation in a dose-dependent manner, as demonstrated by glial-and neuronal-marker expression

Gaucher Disease and Myelofibrosis: A Combined Disease or a Misdiagnosis?

Background: Gaucher disease (GD) and primary myelofibrosis (PMF) share similar clinical and laboratory features, such as cytopenia, hepatosplenomegaly, and marrow fibrosis, often resulting in a misdiagnosis. Case Report: We report here the case of a young woman with hepatosplenomegaly, leukopenia, and thrombocytopenia. Based on bone marrow (BM) findings and on liver biopsy showing extramedullary hematopoiesis, an initial diagnosis of PMF was formulated. The patient refused stem cell transplantation from an HLA-identical sibling. Low-dose melphalan was given, without any improvement. Two years later, a BM evaluation showed Gaucher cells. Low glucocerebrosidase and high chitotriosidase levels were indicative for GD. Molecular analysis revealed N370S/complex I mutations. Enzyme replacement therapy with imiglucerase was commenced, resulting in clinical and hematological improvements. Due to an unexpected and persistent organomegaly, PMF combined with GD were suspected. JAK2V617F, JAK2 exon 12, MPL, calreticulin, and exon 9 mutations were negative, and BM examination showed no marrow fibrosis. PMF was excluded. Twenty years after starting treatment, the peripheral cell count and liver size were normal, whereas splenomegaly persisted. Conclusion: In order to avoid a misdiagnosis, a diagnostic algorithm for patients with hepatosplenomegaly combined with cytopenia is suggested

Dystonia: sparse synapses for D2 receptors in striatum of a DYT1 knock-out mouse model

Dystonia pathophysiology has been partly linked to downregulation and dysfunction of dopamine D2 receptors in striatum. We aimed to investigate the possible morpho-structural correlates of D2 receptor downregulation in the striatum of a DYT1 Tor1a mouse model. Adult control Tor1a+/+ and mutant Tor1a+/− mice were used. The brains were perfused and free-floating sections of basal ganglia were incubated with polyclonal anti-D2 antibody, followed by secondary immune-fluorescent antibody. Confocal microscopy was used to detect immune-fluorescent signals. The same primary antibody was used to evaluate D2 receptor expression by western blot. The D2 receptor immune-fluorescence appeared circumscribed in small disks (~0.3–0.5 μm diameter), likely representing D2 synapse aggregates, densely distributed in the striatum of Tor1a+/+ mice. In the Tor1a+/− mice the D2 aggregates were significantly smaller (μm2 2.4 ± SE 0.16, compared to μm2 6.73 ± SE 3.41 in Tor1a+/+) and sparse, with ~30% less number per microscopic field, value correspondent to the amount of reduced D2 expression in western blotting analysis. In DYT1 mutant mice the sparse and small D2 synapses in the striatum may be insufficient to “gate” the amount of presynaptic dopamine release diffusing in peri-synaptic space, and this consequently may result in a timing and spatially larger nonselective sphere of influence of dopamine action

Nucleon generalized polarizabilities within a relativistic Constituent Quark Model

Nucleon generalized polarizabilities are investigated within a relativistic framework, defining such quantities through a Lorentz covariant multipole expansion of the amplitude for virtual Compton scattering. The key physical ingredients in the calculation of the nucleon polarizabilities are the Lorentz invariant reduced matrix elements of the electromagnetic transition current, which can be evaluated from off-energy-shell helicity amplitudes. The evolution of the proton paramagnetic polarizability, $\beta_{para}(q)$, as a function of the virtual-photon three-momentum transfer $q,$ is explicitly evaluated within a relativistic constituent quark model by adopting transition form factors obtained in the light-front formalism. The discussion is focussed on the role played by the effects due to the relativistic approach and to the transition form factors, derived within different models.Comment: 14 pages and three figures (included), to appear in Phys. Rev. C (May 1998

Splitting of the pi - rho spectrum in a renormalized light-cone QCD-inspired model

We show that the splitting between the light pseudo-scalar and vector meson states is due to the strong short-range attraction in the ^1S_0 sector which makes the pion and the kaon light particles. We use a light-cone QCD-inspired model of the mass squared operator with harmonic confinement and a Dirac-delta interaction. We apply a renormalization method to define the model, in which the pseudo-scalar ground state mass fixes the renormalized strength of the Dirac-delta interaction.Comment: 9 pages, 2 figures, revtex, accepted by Phys. Rev. D; Corrected typo

Electromagnetic form factors in the light-front formalism and the Feynman triangle diagram: spin-0 and spin-1 two-fermion systems

The connection between the Feynman triangle diagram and the light-front formalism for spin-0 and spin-1 two-fermion systems is analyzed. It is shown that in the limit q+ = 0 the form factors for both spin-0 and spin-1 systems can be uniquely determined using only the good amplitudes, which are not affected by spurious effects related to the loss of rotational covariance present in the light-front formalism. At the same time, the unique feature of the suppression of the pair creation process is maintained. Therefore, a physically meaningful one-body approximation, in which all the constituents are on their mass-shells, can be consistently formulated in the limit q+ = 0. Moreover, it is shown that the effects of the contact term arising from the instantaneous propagation of the active constituent can be canceled out from the triangle diagram by means of an appropriate choice of the off-shell behavior of the bound state vertexes; this implies that in case of good amplitudes the Feynman triangle diagram and the one-body light-front result match exactly. The application of our covariant light-front approach to the evaluation of the rho-meson elastic form factors is presented.Comment: corrected typos in the reference

Comparison among Hamiltonian light-front formalisms at q+ = 0 and q+ <> 0: space-like elastic form factors of pseudoscalar and vector mesons

The electromagnetic elastic form factors of pseudoscalar and vector mesons are analyzed for space-like momentum transfers in terms of relativistic quark models based on the Hamiltonian light-front formalism elaborated in different reference frames (q+ 0 and q+ 0). As far as the one-body approximation for the electromagnetic current operator is concerned, it is shown that the predictions of the light-front approach at q+=0 should be preferred, particularly in case of light hadrons, because of: i) the relevant role played by the Z-graph at q+ 0, and ii) the appropriate elimination of spurious effects, related to the orientation of the null hyperplane where the light-front wave function is defined.Comment: version to appear in Phys. Rev. C. No change in the results and in the conclusion

A connection between inclusive semileptonic decays of bound and free heavy quarks

A relativistic constituent quark model, formulated on the light-front, is used to derive a new parton approximation for the inclusive semileptonic decay width of the B-meson. A simple connection between the decay rate of a free heavy-quark and the one of a heavy-quark bound in a meson or in a baryon is established. The main features of the new approach are the treatment of the b-quark as an on-mass-shell particle and the inclusion of the effects arising from the b-quark transverse motion in the B-meson. In a way conceptually similar to the deep-inelastic scattering case, the B-meson inclusive width is expressed as the integral of the free b-quark partial width multiplied by a bound-state factor related to the b-quark distribution function in the B-meson. The non-perturbative meson structure is described through various quark-model wave functions, constructed via the Hamiltonian light-front formalism using as input both relativized and non-relativistic potential models. A link between spectroscopic quark models and the B-meson decay physics is obtained in this way. Our predictions for the B -> X_c l nu_l and B -> X_u l nu_l decays are used to extract the CKM parameters |V_cb| and |V_ub| from available inclusive data. After averaging over the various quark models adopted and including leading-order perturbative QCD corrections, we obtain |V_cb| = (43.0 +/- 0.7_exp +/- 1.8_th) 10^-3 and |V_ub| = (3.83 +/- 0.48_exp +/- 0.14_th) 10^-3, implying |V_ub / V_cb| = 0.089 +/- 0.011_exp +/- 0.005_th, in nice agreement with existing predictions.Comment: revised version with pQCD corrections included, to appear in Physical Review

Rare exclusive semileptonic b -> s transitions in the Standard Model

We study long-distance effects in rare exclusive semileptonic decays B -> (K, K*) (l+ l-, nu bar{nu}) and analyze dilepton spectra and asymmetries within the framework of the Standard Model. The form factors, describing the meson transition amplitudes of the effective Hamiltonian are calculated within the lattice-constrained dispersion quark model: the form factors are given by dispersion representations through the wave functions of the initial and final mesons, and these wave functions are chosen such that the B -> K* transition form factors agree with the lattice results at large q**2. We calculate branching ratios of semileptonic B -> K, K* transition modes and study the sensitivity of observables to the long-distance contributions. The shape of the forward-backward asymmetry and the longitudinal lepton polarization asymmetry are found to be independent of the long-distance effects and mainly determined by the values of the Wilson coefficients in the Standard Model.Comment: revtex, 17 pp., 5 figures with epsfig.st