Legumes for Organic Cropping Systems

A field experiment which focuses on the yield performance of both durum wheat and sunflower included in a twoyear rotation between wheat/subterranean clover and sunflower was carried out in an organic farm in Central Italy. Three factors were included in the experiment according to a factorial design: a) two different previous crop sequences (four years of alfalfa, four years of annual crops); b) three fertilization levels (without and with animal manure incorporated at 15 or 40 cm depth); c) three durum wheat genotypes (Appulo, Arcangelo and Daunia cvs). The results obtained show that a previous alfalfa meadow allows the succeeding crops (wheat and sunflower) to achieve an appropriate and stable grain yield (about 4 and 3 t ha-1, respectively) even without organic manure for two consecutive cycles. When the durum wheat follows an annual crop sequence, grain yield appears unstable and the different levels of organic fertilizer determine a negative effect in the first cycle and a positive effect in the second cycle. The reseeding capability of sunflower was reduced by animal manure treatments and, consequently, the subclover biomass and its nitrogen content were generally lower in fertilized plots. Anyway, subclover biomass, which was ploughed in after reseeding as a green manure for sunflower, proved to be effective in stabilizing sunflower grain yield, especially in plots without any organic fertilization

Alternative Cropping Systems with Self Reseeding Annual Legumes in a Mediterranean Environment

Self-reseeding winter annual legumes (subclover and snail medic) have the potential to induce a significant shift towards a less energy-intensive and more environmentally friendly management in the modern and specialized cereal cropping systems. Nevertheless their use is practically unknown in mixed stands with winter cereals, where they can contribute to supply nitrogen and to reduce herbicides input. We conceived an alternative cropping system where an annual legume performs, respectively, as living mulch in a winter cereal (winter wheat), as a cover crop after reseeding and as dry mulch for the succeeding summer cereal (corn). Trials carried out at the Tuscia University (Central Italy) in the period 1995-1997 showed that almost all the tested legumes cultivars were able both to grow sufficiently as a living mulch in the wheat and to provide a good re-establishment and an abundant mulch after the wheat harvest for the next corn. Trifolium yanninicum cv Trikkala and Trifolium subterraneum cvs Karridale and Mount Barker ranked first in reseeding capacity (up to 400 seedlings m-2) and mulch production (up to 5 t ha-1). No difference in grain yield between wheat with a living mulch and wheat in pure stand was found when legumes, such as Trifolium yanninicum cvs Trikkala and Larisa, Trifolium brachycalycinum cv Clare, Trifolium subterraneum cv Nungarin and Medicago scutellata cv Kelson, were grown in binary mixture with wheat. The aboveground biomass production of the succeeding irrigated corn crop was largely dependent on the amount of legume dry mulch left upon the ground

Habitat Patch Diversity Evaluation for Sustainability: a Case Study of a Rural Area in Central Italy

Landscape analysis is regarded as a new tool for monitoring and judging land use patterns in terms of sustainability of human activity systems at local level. A case study of evaluation for sustainability based on habitat patch diversity in an ecoregion of Central Italy is presented. In this region, ongoing land use patterns reflect both historical adaptation to local environmental constraints and positive, social-oriented management. More protective land use patterns are mostly widespread in fragile physiographic conditions like those of the mountain areas, where woodland, shrub, and grassland patches are larger and cover more than 90% of the land. This situation is regarded as a positive outcome of the traditional public ownership regime, because public lands amount to more than 70% in the mountain areas. The hilly areas, where public property drops to 28%, presents landscape metrics showing a well balanced situation between agricultural land use and protective native woods and grasslands, which provides a fine-grained and harmonious Mediterranean landscape. In the low-land areas, with anthropic pressure and more favourable conditions for crop productivity, there is much more agricultural land, even if some mitigation in terms of biodiversity maintenance is offered by the presence of hedgerow ecotones. In these areas, landscape analysis is not able to supply meaningful information about cropping system design and practices which can maintain a sustainable level of soil fertility and quality of natural resources and processes, and further analysis at cropping system level should be carried out

SERS, XPS and DFT study of xanthine adsorbed on citrate-stabilized gold nanoparticles

We have studied the adsorption of xanthine, a nucleobase present in human tissue and fluids that is involved in important metabolic processes, on citrate-reduced gold colloidal nanoparticles by means of surface-enhanced Raman scattering (SERS), absorption, and X-ray photoelectron spectroscopy (XPS) measurements, along with density functional theory (DFT) calculations. The citrate anions stabilize the colloidal suspensions by strongly binding the gold nanoparticles. However, these anions do not impair the adsorption of xanthine on positively-charged active sites present on the metal surface. We have obtained the Fourier transform (FT)-SERS spectra of adsorbed xanthine by laser excitation in the near infrared spectral region, where interference due to fluorescence emission does not usually occur. In fact, the addition of chloride ions to the Au/xanthine colloid induces the aggregation of the gold nanoparticles, whose plasmonic band is shifted to the near infrared region where there is the exciting laser line of the FT\u2013Raman instrument. Hence, this analytical approach is potentially suitable for spectroscopic determination of xanthine directly in body fluids, avoiding fluorescence phenomena induced by visible laser irradiation

Regressing multiple viral plaques and skin fragility syndrome in a cat coinfected with FcaPV2 and FcaPV3

Feline viral plaques are uncommon skin lesions clinically characterized by multiple, often pigmented, and slightly raised lesions. Numerous reports suggest that papillomaviruses (PVs) are involved in their development. Immunosuppressed and immunocompetent cats are both affected, the biological behavior is variable, and the regression is possible but rarely documented. Here we report a case of a FIV-positive cat with skin fragility syndrome and regressing multiple viral plaques in which the contemporary presence of two PV types (FcaPV2 and FcaPV3) was demonstrated by combining a quantitative molecular approach to histopathology. The cat, under glucocorticoid therapy for stomatitis and pruritus, developed skin fragility and numerous grouped slightly raised nonulcerated pigmented macules and plaques with histological features of epidermal thickness, mild dysplasia, and presence of koilocytes. Absolute quantification of the viral DNA copies (4555 copies/microliter of FcaPV2 and 8655 copies/microliter of FcaPV3) was obtained. Eighteen months after discontinuation of glucocorticoid therapy skin fragility and viral plaques had resolved.The role of the two viruses cannot be established and it remains undetermined how each of the viruses has contributed to the onset of VP; the spontaneous remission of skin lesions might have been induced by FIV status change over time due to glucocorticoid withdraw and by glucocorticoids withdraw itself

COVID-19 infection and rheumatoid arthritis: Faraway, so close!

The outbreak of the new coronavirus infections COVID-19 in December 2019 in China has quickly become a global health emergency. Given the lack of specific anti-viral therapies, the current management of severe acute respiratory syndrome coronaviruses (SARS-CoV-2) is mainly supportive, even though several compounds are now under investigation for the treatment of this life-threatening disease. COVID-19 pandemic is certainly conditioning the treatment strategy of a complex disorder as rheumatoid arthritis (RA), whose infectious risk is increased compared to the general population because of an overall impairment of immune system typical of autoimmune diseases combined with the iatrogenic effect generated by corticosteroids and immunosuppressive drugs. However, the increasing knowledge about the pathophysiology of SARS-CoV-2 infection is leading to consider some anti-rheumatic drugs as potential treatment options for the management of COVID-19. In this review we will critically analyse the evidences on either positive or negative effect of drugs commonly used to treat RA in this particular scenario, in order to optimize the current approach to RA patients

Taking care of systemic sclerosis patients during COVID-19 pandemic : rethink the clinical activity

COVID-19 outbreak has quickly spread worldwide, causing a high pressure on the health-care system. In Italy, from March 8, 2020, all the deferrable clinical activities have been suspended to increase the health care offer for COVID-19 patients. The hospital organization has been modified also in order to assure non-COVID-19 patients assistance. The Scleroderma Unit of ASST Pini-CTO Hospital, in Milan, in the region mostly hit by SARS-CoV-2 in Italy, follows more than 600 patients affected by systemic sclerosis (SSc). Patients with SSc need a close follow-up with a regular screening of organ involvement and frequent intravenous treatments. All SSc patients have been educated about ministerial directives to limit COVID-19 spread. The organization of our Scleroderma Unit has been quickly rethought to assure SSc patients assistance in safety for them and for health-care workers during urgent visits or infusion therapies. Using electronic way of communication with frequent virtual contact and guarantying home deliveries of some therapies, we allowed a continuity of care also outside the Hospital