TsDAF-21/Hsp90 is expressed in all examined stages of Trichinella spiralis

Trichinella is an important parasitic nematode of animals worldwide. Heat shock proteins are ubiquitous in nature and allow organisms to quickly respond to environmental stress. A portion of the Tsdaf-21 gene, a Caenorhabditis elegans daf-21 homologue encoding heat-shock protein 90 (Hsp90) was cloned from Trichinella spiralis. The partial nucleotide sequence resided near the 5′-end of the gene and encoded a polypeptide of 254 amino acid residues harboring a HATPase-c superfamily domain and Hsp90 protein domain. Phylogenetic analysis revealed that Tsdaf-21 is highly conserved and formed a monophyletic clade with other nematodes. The partial Tsdaf-21 transcript was subcloned and expressed for antibody production. Results using PCR primers specific for the Tsdaf-21 transcript, and mouse polyclonal antisera specific for the recombinant protein showed that both the RNA transcript and the corresponding protein were ubiquitously and consistently expressed in newborn larvae, muscle larvae and both male and female adult worms in the absence of any external stress or stimulation

E2-AEN: End-to-End Incremental Learning with Adaptively Expandable Network

Expandable networks have demonstrated their advantages in dealing with catastrophic forgetting problem in incremental learning. Considering that different tasks may need different structures, recent methods design dynamic structures adapted to different tasks via sophisticated skills. Their routine is to search expandable structures first and then train on the new tasks, which, however, breaks tasks into multiple training stages, leading to suboptimal or overmuch computational cost. In this paper, we propose an end-to-end trainable adaptively expandable network named E2-AEN, which dynamically generates lightweight structures for new tasks without any accuracy drop in previous tasks. Specifically, the network contains a serial of powerful feature adapters for augmenting the previously learned representations to new tasks, and avoiding task interference. These adapters are controlled via an adaptive gate-based pruning strategy which decides whether the expanded structures can be pruned, making the network structure dynamically changeable according to the complexity of the new tasks. Moreover, we introduce a novel sparsity-activation regularization to encourage the model to learn discriminative features with limited parameters. E2-AEN reduces cost and can be built upon any feed-forward architectures in an end-to-end manner. Extensive experiments on both classification (i.e., CIFAR and VDD) and detection (i.e., COCO, VOC and ICCV2021 SSLAD challenge) benchmarks demonstrate the effectiveness of the proposed method, which achieves the new remarkable results

Lineage Divergence and Historical Gene Flow in the Chinese Horseshoe Bat (Rhinolophus sinicus)

PMCID: PMC3581519This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

TsDAF-21/Hsp90 is expressed in all examined stages of Trichinella spiralis

Trichinella is an important parasitic nematode of animals worldwide. Heat shock proteins are ubiquitous in nature and allow organisms to quickly respond to environmental stress. A portion of the Tsdaf-21 gene, a Caenorhabditis elegans daf-21 homologue encoding heat-shock protein 90 (Hsp90) was cloned from Trichinella spiralis. The partial nucleotide sequence resided near the 5′-end of the gene and encoded a polypeptide of 254 amino acid residues harboring a HATPase-c superfamily domain and Hsp90 protein domain. Phylogenetic analysis revealed that Tsdaf-21 is highly conserved and formed a monophyletic clade with other nematodes. The partial Tsdaf-21 transcript was subcloned and expressed for antibody production. Results using PCR primers specific for the Tsdaf-21 transcript, and mouse polyclonal antisera specific for the recombinant protein showed that both the RNA transcript and the corresponding protein were ubiquitously and consistently expressed in newborn larvae, muscle larvae and both male and female adult worms in the absence of any external stress or stimulation

Wireless Power Transfer Positioning System with Wide Range Direction-Guided Based on Symmetrical Triple-U Auxiliary Pad

An important area of research in electric vehicle wireless power transfer systems is the detection of the secondary pad, which is vital evidence to determine whether the vehicle is in the effective charging area. However, the detection based on sensors mostly will reconstruct the vehicle structure and has a limit on versatility in all kinds of vehicles and the applicability of magnetic couplers and the influence on the primary pad. Therefore, an auxiliary pad structure and corresponding positioning method for offset estimation utilizing the existing inverter and secondary pad in the vehicle system are proposed. Firstly, to satisfy the needs of different positioning heights and avoid the effect on the primary pad, a triple-U positioning auxiliary pad is designed to assist positioning. Secondly, the direction-guided trajectory and detection algorithm are proposed to modify the vehicle location in real-time after analyzing the corresponding equivalent mutual inductance feature trajectory, according to the magnetic field characteristics of various typical magnetic couplers intervened by the proposed triple-U auxiliary pad. Finally, a prototype system is built to validate the applicability and feasibility of the triple-U auxiliary pad, where the positioning accuracy is within 10 mm, and the maximum recognizable recognition range can reach 300 mm × 300 mm, and the direction-guided trajectory is accurate, which can satisfy the actual positioning requirements of electric vehicles

TsDAF-21/Hsp90 is expressed in all examined stages of Trichinella spiralis

Conference Name:13th International Conference on Trichinellosis. Conference Address: Changchun, PEOPLES R CHINA. Time:AUG 01-06, 2011.Natural Science Foundation of China [30972181]; Xiamen Science Technology program [3502Z20061098]Trichinella is an important parasitic nematode of animals worldwide. Heat shock proteins are ubiquitous in nature and allow organisms to quickly respond to environmental stress. A portion of the Tsdaf-21 gene, a Caenorhabditis elegans daf-21 homologue encoding heat-shock protein 90 (Hsp90) was cloned from Trichinella spiralis. The partial nucleotide sequence resided near the 5'-end of the gene and encoded a polypeptide of 254 amino acid residues harboring a HATPase-c superfamily domain and Hsp90 protein domain. Phylogenetic analysis revealed that Tsdaf-21 is highly conserved and formed a monophyletic clade with other nematodes. The partial Tsdaf-21 transcript was subcloned and expressed for antibody production. Results using PCR primers specific for the Tsdaf-21 transcript, and mouse polyclonal antisera specific for the recombinant protein showed that both the RNA transcript and the corresponding protein were ubiquitously and consistently expressed in newborn larvae, muscle larvae and both male and female adult worms in the absence of any external stress or stimulation. (C) 2013 Elsevier B.V. All rights reserved

RETRACTED ARTICLE: Syndecan-1 suppresses cell growth and migration via blocking JAK1/STAT3 and Ras/Raf/MEK/ERK pathways in human colorectal carcinoma cells

Abstract Background Syndecan-1 (SDC-1) is a crucial membrane proteoglycan, which is confirmed to participate in several tumor cell biological processes. However, the biological significance of SDC-1 in colorectal carcinoma is not yet clear. An objective of this study was to investigate the role of SDC-1 in colorectal carcinoma cells. Methods Expression of SDC-1 in colorectal carcinoma tissues was evaluated by Reverse transcription-quantitative real-time PCR (RT-qPCR) and western blot. After transfection with pcDNA3.1 or pc-SDC-1, the transfection efficiency was measured. Next, SW480, SW620 and LOVO cell viability, apoptosis, migration and adhesion were assessed to explore the effects of exogenous overexpressed SDC-1 on colorectal carcinoma. In addition, the influences of aberrant expressed SDC-1 in Janus kinase 1 (JAK1)/signal transducer and activator of transcription 3 (STAT3) and rat sarcoma virus (Ras)/rapidly accelerated fibrosarcoma (Raf)/mitogen-activated protein kinase (MEK)/extracellular signal-regulated kinase (ERK) pathways were detected by western blot analysis. Results SDC-1 mRNA and protein levels were down-regulated in human colorectal carcinoma tissues. SDC-1 overexpression inhibited cell proliferation via suppressing CyclinD1 and c-Myc expression, meanwhile stimulated cell apoptosis via increasing the expression levels of B-cell lymphoma-2-associated x (Bax) and Cleaved-Caspase-3. Additionally, SDC-1 overexpression restrained cell migration via inhibiting the protein expression of matrix metallopeptidase 9 (MMP-9), and elicited cell adhesion through increasing intercellular cell adhesion molecule-1 (ICAM-1). Furthermore, SDC-1 overexpression suppressed JAK1/STAT3 and Ras/Raf/MEK/ERK-related protein levels. Conclusions In general, the evidence from this study suggested that SDC-1 suppressed cell growth, migration through blocking JAK1/STAT3 and Ras/Raf/MEK/ERK pathways in human colorectal carcinoma cells

Rapid Screening and Identification of Antitumor Ingredients from the Mangrove Endophytic Fungus Using an Enzyme-Immobilized Magnetic Nanoparticulate System

As a consequence of recent progression in biomedicine and nanotechnology, nanoparticle-based systems have evolved as a new method with extensive applications in responsive therapy, multimodal imaging, drug delivery and natural product separation. Meanwhile, the magnetic nanoparticulate system has aroused great interest for separation and purification because of its excellent magnetic properties. Phospholipase A2 (PLA2) is a highly expressed regulator to promote the growth of various cancers and is an ideal target to treat cancers. In this study, a novel strategy based on ligand–receptor interactions to discover novel PLA2 inhibitors was established, in which PLA2-functionalized Fe3O4@PLGA-PEG-NH2 magnetic nanoparticles were used as a supporting material combined with high-performance liquid chromatography–mass spectrometry, aiming to accelerate the discovery of novel PLA2 inhibitors from natural sources such as mangrove endophytic fungi. Under the optimized ligand fishing conditions, six target compounds were ultimately fished and identified to be cyclic peptides (1–3) and sterols (4–6), which compounds 1, 2 and 4–6 have well-documented cytotoxicities. Compound 3 exerted better inhibitory effect on A549 cells by experiment. In conclusion, PLA2-functionalized Fe3O4@PLGA-PEG-NH2 magnetic nanoparticles-based ligand fishing provided a feasible, selective and effective platform for the efficient screening and identification of antitumor components from natural products