On the Lab. II building site

425 special CDSs in PCN033 (XLSX 29 kb

Preparation and thermal conductivity of CuO nanofluid via a wet chemical method

In this article, a wet chemical method was developed to prepare stable CuO nanofluids. The influences of synthesis parameters, such as kinds and amounts of copper salts, reaction time, were studied. The thermal conductivities of CuO nanofluids were also investigated. The results showed that different copper salts resulted in different particle morphology. The concentration of copper acetate and reaction time affected the size and shape of clusters of primary nanoparticles. Nanofluids with different microstructures could be obtained by changing the synthesis parameters. The thermal conductivities of CuO nanofluids increased with the increase of particle loading

A fast reciprocal space method for image simulation

schedatura sistematica dei siti preromani del veronese a ovest e sud dell'Adig

Whole genome sequence and comparative genome analyses of multi-resistant Staphylococcus warneri GD01 isolated from a diseased pig in China.

Staphylococcus warneri is a coagulase-negative staphylococcus that is a normal inhabitant of the skin. It is also considered to be an opportunistic etiological agent causing significant infections in human and animals. Currently, relatively little attention has been paid to the genome biology of S. warneri pathogenicity and antibiotic resistance, which are emerging issues for this etiological agent with considerably clinical significance. In this study, we determined the complete genome sequence of S. warneri strain GD01 recovered from the sampled muscle abscess tissue of a diseased pig in South China. The genome of S. warneri is composed of a circular chromosome of 2,473,911 base pairs as well as eight plasmid sequences. Genome-wide metabolic reconstruction revealed 82 intact functional modules driving the catabolism of respiration and fermentation for energy production, uptake of distinct sugars as well as two-component regulatory systems. The evidence uncovered herein enables better understanding for metabolic potential and physiological traits of this etiological agent. The antibiotic susceptibility test demonstrated that S. warneri GD01 was resistant to penicillin, amoxicillin, ampicillin, cefalexin, vancomycin, and sulfisoxazole. The associations between antibiotic phenotypes and the related genotypes were identified to reveal the molecular basis conferring resistance to this pathogen. A number of genes coding for potential virulence factors were firstly depicted in the genome of S. warneri GD01, including adhesins, exoenzymes, capsule, and iron acquisition proteins. Our study provides a valuable genomic context of the genes/modules devoting to metabolism, antibiotic resistance, and virulence of S. warneri

Influence of legacy soil map accuracy on soil map updating with data mining methods

Over the past decades, conventional soil maps of various scales have been produced and become available in digital form. Efforts have been made to update these maps through various data mining methods to provide more detailed and precise information on soil spatial patterns. Key questions that remain unclear are: (1) How does the accuracy of legacy soil maps impact the update results; (2) Is the accuracy of inferred soil maps always improved regardless of the accuracy of the legacy maps. The current study aims to investigate these questions. Two noise production simulation methods were developed to simulate errors caused by inclusion and boundary displacement in the conventional maps, to generate a series of source maps with different accuracies and spatial patterns. Moreover, the impacts of two training sample selection methods and three data mining models on the accuracies and spatial patterns of the inferred soil maps were also evaluated. A case study was conducted in a small region, Raffelson study area, a typical ridge and valley terrain in La Crosse County, Wisconsin, USA. Results indicated that if the accuracies of the source soil maps ranged from 35% to 75%, the inferred soil map accuracies would be improved. These findings have important implications for updating conventional soil maps through data mining methods and understanding the situation in which the method is effective

Increased Mobile Zinc Regulates Retinal Ganglion Cell Survival via Activating Mitochondrial OMA1 and Integrated Stress Response

Retinal ganglion cells (RGCs), the projection neurons of the eye, are irreversibly lost once the optic nerve is injured, which is a critical mechanism of glaucoma. Mobile zinc (Zn2+) levels rapidly increase in retinal interneuron amacrine cells and Zn2+ is then transferred to RGCs via the Zn2+ transporter protein ZnT-3, triggering RGC loss in optic nerve injury. Zn2+ chelation and ZnT-3 deletion promote long-term RGC survival. However, the downstream signaling pathways of Zn2+ in RGCs remains unknown. Here, we show that increased levels of Zn2+ upregulate the expression and activity of mitochondrial zinc metallopeptidase OMA1 in the retina, leading to the cleavage of DELE1 and activation of cytosolic eIF2α kinase PKR, triggering the integrated stress response (ISR) in RGCs. Our study identified OMA1 and ISR as the downstream molecular mechanisms of retinal Zn2+ and potential targets for preventing the progression of Zn2+-associated neuronal damage

Additional file 2: Table S2. of Genome analysis and in vivo virulence of porcine extraintestinal pathogenic Escherichia coli strain PCN033

Core genome content used to construct phylogenetic tree (XLSX 225 kb