Calcium Transport and Local Pool Regulate Polycystin-2 (TRPP2) Function in Human Syncytiotrophoblast

AbstractPolycystin-2 (PC2, TRPP2) is a Ca2+-permeable, nonselective cation channel implicated in Ca2+ transport and epithelial cell signaling. Although PC2 may contribute to Ca2+ transport in human term placenta, the regulatory mechanisms associated with Ca2+ handling in this tissue are largely unknown. In this work we assessed the regulation by Ca2+ of PC2 channel function from a preparation of apical membranes of human syncytiotrophoblast (PC2hst) reconstituted in a lipid bilayer system. Addition of either EGTA or BAPTA to the cis hemi-chamber, representing the cytoplasmic domain of the channel, and lowering Ca2+ to ∼0.6–0.8 nM, inhibited spontaneous PC2hst channel activity, with a time response dependent on the chelator tested. EGTA reduced PC2hst channel currents by 86%, with a t1/2 = 3.6 min, whereas BAPTA rapidly and completely (100%) eliminated channel activity with a t1/2 = 0.8 min. Subsequent titration with Ca2+ reversed the inhibition, which followed a Hill-type function with apparent dissociation constants of 1–5 nM, and 4 Ca2+ binding sites. The degree of inhibition by the cis Ca2+ chelator largely depended on increasing trans Ca2+. This was consistent with measurable Ca2+ transport through the channel, feeding the regulatory sites in the cytoplasmic domain. Interestingly, the reconstituted in vitro translated PC2 (PC2iv) was completely insensitive to Ca2+ regulation, suggesting that the regulatory sites are not intrinsic to the channel protein. Our findings demonstrate the presence of a Ca2+ microdomain largely accessible through the channel that controls PC2 function in human syncytiotrophoblast of term placenta

Modules for Experiments in Stellar Astrophysics (MESA): Giant Planets, Oscillations, Rotation, and Massive Stars

We substantially update the capabilities of the open source software package Modules for Experiments in Stellar Astrophysics (MESA), and its one-dimensional stellar evolution module, MESA Star. Improvements in MESA Star's ability to model the evolution of giant planets now extends its applicability down to masses as low as one-tenth that of Jupiter. The dramatic improvement in asteroseismology enabled by the space-based Kepler and CoRoT missions motivates our full coupling of the ADIPLS adiabatic pulsation code with MESA Star. This also motivates a numerical recasting of the Ledoux criterion that is more easily implemented when many nuclei are present at non-negligible abundances. This impacts the way in which MESA Star calculates semi-convective and thermohaline mixing. We exhibit the evolution of 3-8 Msun stars through the end of core He burning, the onset of He thermal pulses, and arrival on the white dwarf cooling sequence. We implement diffusion of angular momentum and chemical abundances that enable calculations of rotating-star models, which we compare thoroughly with earlier work. We introduce a new treatment of radiation-dominated envelopes that allows the uninterrupted evolution of massive stars to core collapse. This enables the generation of new sets of supernovae, long gamma-ray burst, and pair-instability progenitor models. We substantially modify the way in which MESA Star solves the fully coupled stellar structure and composition equations, and we show how this has improved MESA's performance scaling on multi-core processors. Updates to the modules for equation of state, opacity, nuclear reaction rates, and atmospheric boundary conditions are also provided. We describe the MESA Software Development Kit (SDK) that packages all the required components needed to form a unified and maintained build environment for MESA. [Abridged]Comment: Accepted for publication in The ApJ Supplement Series. Extra informations required to reproduce the calculations in this paper are available at http://mesastar.org/results/mesa

The Fornax Deep Survey with VST. I. The extended and diffuse stellar halo of NGC~1399 out to 192 kpc

[Abrigded] We have started a new deep, multi-imaging survey of the Fornax cluster, dubbed Fornax Deep Survey (FDS), at the VLT Survey Telescope. In this paper we present the deep photometry inside two square degrees around the bright galaxy NGC1399 in the core of the cluster. We found a very extended and diffuse envelope surrounding the luminous galaxy NGC1399: we map the surface brightness out to 33 arcmin (~ 192 kpc) from the galaxy center and down to about 31 mag/arcsec^2 in the g band. The deep photometry allows us to detect a faint stellar bridge in the intracluster region between NGC1399 and NGC1387. By analyzing the integrated colors of this feature, we argue that it could be due to the ongoing interaction between the two galaxies, where the outer envelope of NGC1387 on its east side is stripped away. By fitting the light profile, we found that it exists a physical break radius in the total light distribution at R=10 arcmin (~58 kpc) that sets the transition region between the bright central galaxy and the outer exponential stellar halo. We discuss the main implications of this work on the build-up of the stellar halo at the center of the Fornax cluster. By comparing with the numerical simulations of the stellar halo formation for the most massive BCGs, we find that the observed stellar halo mass fraction is consistent with a halo formed through the multiple accretion of progenitors with a stellar mass in the range 10^8 - 10^11 M_sun. This might suggest that the halo of NGC1399 has also gone through a major merging event. The absence of a significant number of luminous stellar streams and tidal tails out to 192 kpc suggests that the epoch of this strong interaction goes back to an early formation epoch. Therefore, differently from the Virgo cluster, the extended stellar halo around NGC1399 is characterised by a more diffuse and well-mixed component, including the ICL.Comment: Accepted for publication in ApJ; 25 pages and 14 figures. An higher resolution file is available at the following link https://www.dropbox.com/s/fvltppduysdn6pb/NGC1399_fin_2c.pdf?dl=

A Bio-Polymer Transistor: Electrical Amplification by Microtubules

Microtubules (MTs) are important cytoskeletal structures, engaged in a number of specific cellular activities, including vesicular traffic, cell cyto-architecture and motility, cell division, and information processing within neuronal processes. MTs have also been implicated in higher neuronal functions, including memory, and the emergence of "consciousness". How MTs handle and process electrical information, however, is heretofore unknown. Here we show new electrodynamic properties of MTs. Isolated, taxol-stabilized microtubules behave as bio-molecular transistors capable of amplifying electrical information. Electrical amplification by MTs can lead to the enhancement of dynamic information, and processivity in neurons can be conceptualized as an "ionic-based" transistor, which may impact among other known functions, neuronal computational capabilities.Comment: This is the final submitted version. The published version should be downloaded from Biophysical Journa

Thermohaline mixing and the photospheric composition of low-mass giant stars

We compute full evolutionary sequences of red giant branch stars close to the luminosity bump by including state of the art composition transport prescriptions for the thermohaline mixing regimes. In particular we adopt a self-consistent double-diffusive convection theory, that allows to handle the instabilities that arise when thermal and composition gradients compete against each other, and a very recent empirically motivated and parameter free asymptotic scaling law for thermohaline composition transport. In agreement with previous works, we find that during the red giant stage, a thermohaline instability sets in shortly after the hydrogen burning shell (HBS) encounters the chemical discontinuity left behind by the first dredge-up. We also find that the thermohaline unstable region, initially appearing at the exterior wing of the HBS, is unable to reach the outer convective envelope, with the consequence that no mixing of elements that produces a non-canonical modification of the stellar surface abundances occurs. Also in agreement with previous works, we find that by artificially increasing the mixing efficiency of thermohaline regions it is possible to connect both unstable regions, thus affecting the photospheric composition. However, we find that in order to reproduce the observed abundances of red giant branch stars close to the luminosity bump, thermohaline mixing efficiency has to be artificially increased by about 4 orders of magnitude from that predicted by recent 3D numerical simulations of thermohaline convection close to astrophysical environments. From this we conclude the chemical abundance anomalies of red giant stars cannot be explained on the basis of thermohaline mixing alone.Comment: 7 pages, 6 figures, accepted for publication in A&

The Fornax Deep Survey with VST. II. Fornax A: a two-phase assembly caught on act

As part of the Fornax Deep Survey with the ESO VLT Survey Telescope, we present new $g$ and $r$ bands mosaics of the SW group of the Fornax cluster. It covers an area of $3 \times 2$ square degrees around the central galaxy NGC1316. The deep photometry, the high spatial resolution of OmegaCam and the large covered area allow us to study the galaxy structure, to trace stellar halo formation and look at the galaxy environment. We map the surface brightness profile out to 33arcmin ($\sim 200$kpc $\sim15R_e$) from the galaxy centre, down to $\mu_g \sim 31$ mag arcsec$^{-2}$ and $\mu_r \sim 29$ mag arcsec$^{-2}$. This allow us to estimate the scales of the main components dominating the light distribution, which are the central spheroid, inside 5.5 arcmin ($\sim33$ kpc), and the outer stellar envelope. Data analysis suggests that we are catching in act the second phase of the mass assembly in this galaxy, since the accretion of smaller satellites is going on in both components. The outer envelope of NGC1316 still hosts the remnants of the accreted satellite galaxies that are forming the stellar halo. We discuss the possible formation scenarios for NGC1316, by comparing the observed properties (morphology, colors, gas content, kinematics and dynamics) with predictions from cosmological simulations of galaxy formation. We find that {\it i)} the central spheroid could result from at least one merging event, it could be a pre-existing early-type disk galaxy with a lower mass companion, and {\it ii)} the stellar envelope comes from the gradual accretion of small satellites.Comment: Accepeted for publication in Ap