Receptors and channels possibly mediating the effects of phytocannabinoids on seizures and epilepsy

Epilepsy contributes to approximately 1% of the global disease burden. By affecting especially young children as well as older persons of all social and racial variety, epilepsy is a present disorder worldwide. Currently, only 65% of epileptic patients can be successfully treated with antiepileptic drugs. For this reason, alternative medicine receives more attention. Cannabis has been cultivated for over 6000 years to treat pain and insomnia and used since the 19th century to suppress epileptic seizures. The two best described phytocannabinoids, (−)-trans-Δ9- tetrahydrocannabinol (THC) and cannabidiol (CBD) are claimed to have positive effects on different neurological as well as neurodegenerative diseases, including epilepsy. There are different cannabinoids which act through different types of receptors and channels, including the cannabinoid receptor 1 and 2 (CB1, CB2), G protein-coupled receptor 55 (GPR55) and 18 (GPR18), opioid receptor μ and δ, transient receptor potential vanilloid type 1 (TRPV1) and 2 (TRPV2), type A γ-aminobutyric acid receptor (GABAAR) and voltage-gated sodium channels (VGSC). The mechanisms and importance of the interaction between phytocannabinoids and their different sites of action regarding epileptic seizures and their clinical value are described in this review

Cannabinoid profiling of hemp seed oil by liquid chromatography coupled to high-resolution mass spectrometry

Hemp seed oil is well known for its nutraceutical, cosmetic and pharmaceutical properties due to a perfectly balanced content of omega 3 and omega 6 polyunsaturated fatty acids. Its importance for human health is reflected by the success on the market of organic goods in recent years. However, it is of utmost importance to consider that its healthy properties are strictly related to its chemical composition, which varies depending not only on the manufacturing method, but also on the hemp variety employed. In the present work, we analyzed the chemical profile of ten commercially available organic hemp seed oils. Their cannabinoid profile was evaluated by a liquid chromatography method coupled to high-resolution mass spectrometry. Besides tetrahydrocannabinol and cannabidiol, other 30 cannabinoids were identified for the first time in hemp seed oil. The results obtained were processed according to an untargeted metabolomics approach. The multivariate statistical analysis showed highly significant differences in the chemical composition and, in particular, in the cannabinoid content of the hemp oils under investigation

Practical implementation of diffused sensing elements for TDR-based monitoring of rising damp in building structures

This paper describes the operating and technical details of the practical implementation of an innovative time domain reflectometry (TDR)-system for monitoring rising damp in building structures. The proposed system employs wire-like, passive, diffused sensing elements (SE's) that are embedded, at the time of construction or renovation, inside the walls of the building to be monitored. The SE's remain permanently inside the wall, ready to be interrogated when necessary

Moisture content measurements through TDR: A metrological assessment for industrial applications

In this paper a metrological assessment on the accuracy provided by a Time Domain Reflectometry (TDR)-based method for the estimation of moisture content of granular materials is proposed. In particular, comparative moisture content measurements are carried out through two different TDR instruments: an inexpensive portable unit and a high-performance unit. The main goals are first to assess a robust procedure for TDR moisture monitoring (in particular for sand-like materials), and second to provide a deep metrological analysis for minimizing and characterizing error contributions. This feature is particularly important when considering the proposed measurement procedures for industrial applications, where both accuracy and low cost must be guaranteed

Accuracy improvement in the TDR-based localization of water leaks

A time domain reflectometry (TDR)-based system for the localization of water leaks has been recently developed by the authors. This system, which employs wire-like sensing elements to be installed along the underground pipes, has proven immune to the limitations that affect the traditional, acoustic leak-detection systems. Starting from the positive results obtained thus far, in this work, an improvement of this TDR-based system is proposed. More specifically, the possibility of employing a low-cost, water-absorbing sponge to be placed around the sensing element for enhancing the accuracy in the localization of the leak is addressed. To this purpose, laboratory experiments were carried out mimicking a water leakage condition, and two sensing elements (one embedded in a sponge and one without sponge) were comparatively used to identify the position of the leak through TDR measurements. Results showed that, thanks to the water retention capability of the sponge (which maintains the leaked water more localized), the sensing element embedded in the sponge leads to a higher accuracy in the evaluation of the position of the leak

Accuracy improvement in the TDR-based localization of water leaks

A time domain reflectometry (TDR)-based system for the localization of water leaks has been recently developed by the authors. This system, which employs wire-like sensing elements to be installed along the underground pipes, has proven immune to the limitations that affect the traditional, acoustic leak-detection systems.Starting from the positive results obtained thus far, in this work, an improvement of this TDR-based system is proposed. More specifically, the possibility of employing a low-cost, water-absorbing sponge to be placed around the sensing element for enhancing the accuracy in the localization of the leak is addressed.To this purpose, laboratory experiments were carried out mimicking a water leakage condition, and two sensing elements (one embedded in a sponge and one without sponge) were comparatively used to identify the position of the leak through TDR measurements. Results showed that, thanks to the water retention capability of the sponge (which maintains the leaked water more localized), the sensing element embedded in the sponge leads to a higher accuracy in the evaluation of the position of the leak. Keywords: Leak localization, TDR, Time domain reflectometry, Water leaks, Underground water pipe

On the use of dielectric spectroscopy for quality control of vegetable oils

Quality control of vegetable oils is becoming more stringent, and related laws are being enforced especially for avoiding adulteration. As a result, there is a substantial need for methods of analysis that could provide real-time in-situ monitoring, especially for quality control purposes during production process. In this regard, the present paper investigates the possibility of monitoring qualitative characteristics of vegetable oils through microwave dielectric spectroscopy, which is a highly versatile investigative approach. In particular, the Cole & Cole frequency-domain dielectric parameters are known to be strongly related to the compositional characteristics of various substances. This way, starting from traditional Time Domain Reflectometry measurements performed on oils, the corresponding frequency domain information is retrieved. Successively, through a minimization routine, the Cole & Cole parameters of each considered oil are extrapolated. Results show that different dielectric characteristics can be associated with different oils. It is important to point out that the characteristics of the proposed procedure can be automated and, therefore, it may represent a promising solution for practical monitoring applications

Kratom: The analytical challenge of an emerging herbal drug

Mitragyna speciosa or kratom is emerging worldwide as a “legal” herbal drug of abuse. An increasing number of papers is appearing in the scientific literature regarding its pharmacological profile and the analysis of its chemical constituents, mainly represented by alkaloids. However, its detection and identification are not straightforward as the plant material is not particularly distinctive. Hyphenated techniques are generally preferred for the identification and quantification of these compounds, especially the main purported psychoactive substances, mitragynine (MG) and 7-hydroxymitragynine (7-OH-MG), in raw and commercial products. Considering the vast popularity of this recreational drug and the growing concern about its safety, the analysis of alkaloids in biological specimens is also of great importance for forensic and toxicological laboratories. The review addresses the analytical aspects of kratom spanning the extraction techniques used to isolate the alkaloids, the qualitative and quantitative analytical methods and the strategies for the distinction of the naturally occurring isomers

An inverse validation for detecting pipe leaks with a TDR-based method

Recently, an innovative system based on time domain reflectometry (TDR) for the individuation of leaks in underground pipes has been proposed and validated. Starting from the results obtained so far, the present works aims at further investigating the practical applicability of the aforementioned system. In particular, the goal of this work is to assess the system in the detection of two close leaks (i.e. leakages that may occur on the same length of pipe). To this purpose, an experimental setup was arranged: two "leakage conditions" were imposed, and the position of the leaks were considered as unknown and calculated through the dedicated developed algorithm. Results show that, differently from traditional leak detection methods (in which the presence of a leak may "mask" the presence of other leaks), the TDR-based system successfully individuates and correctly localizes the presence of two leaks

Reflectometric System for Continuous and Automated Monitoring of Irrigation in Agriculture

In this work, a time domain reflectometry (TDR)-based system for continuous and diffused monitoring of soil water content in agriculture is presented. The proposed TDR-based system employs elongate sensing elements (SEs). In practical application, each wire-like SE is buried along the cultivation row to be monitored, and through a single TDR measurement it is possible to retrieve the water content profile of the cultivation along the length of the SE. By connecting the TDR-based monitoring system to the irrigation machines, it would be possible to automatically start/stop irrigation based on the actual water requirement of the cultivations, thus favoring precision agriculture and enhancing irrigation efficiency. To demonstrate the feasibility of the proposed monitoring solution, a dedicated hardware+software platform was developed and the TDR-based system was experimented in open-field cultivations