Inferring spatial and signaling relationships between cells from single cell transcriptomic data.

Single-cell RNA sequencing (scRNA-seq) provides details for individual cells; however, crucial spatial information is often lost. We present SpaOTsc, a method relying on structured optimal transport to recover spatial properties of scRNA-seq data by utilizing spatial measurements of a relatively small number of genes. A spatial metric for individual cells in scRNA-seq data is first established based on a map connecting it with the spatial measurements. The cell-cell communications are then obtained by "optimally transporting" signal senders to target signal receivers in space. Using partial information decomposition, we next compute the intercellular gene-gene information flow to estimate the spatial regulations between genes across cells. Four datasets are employed for cross-validation of spatial gene expression prediction and comparison to known cell-cell communications. SpaOTsc has broader applications, both in integrating non-spatial single-cell measurements with spatial data, and directly in spatial single-cell transcriptomics data to reconstruct spatial cellular dynamics in tissues

Applications of Fixed Point Theorems to the Vacuum Einstein Constraint Equations with Non-Constant Mean Curvature

In this paper, we introduce new methods for solving the vacuum Einstein constraints equations: the first one is based on Schaefer's fixed point theorem (known methods use Schauder's fixed point theorem) while the second one uses the concept of half-continuity coupled with the introduction of local supersolutions. These methods allow to: unify some recent existence results, simplify many proofs (for instance, the main theorem in arXiv:1012.2188) and weaken the assumptions of many recent results.Comment: In this version, I change from 3-dimensional case to n-dimensional cas