The level and duration of RSV-specific maternal IgG in infants in Kilifi Kenya

Background Respiratory syncytial virus (RSV) is the major cause of lower respiratory tract infection in infants. The rate of decay of RSV-specific maternal antibodies (RSV-matAb), the factors affecting cord blood levels, and the relationship between these levels and protection from infection are poorly defined. Methods A birth cohort (n = 635) in rural Kenya, was studied intensively to monitor infections and describe age-related serological characteristics. RSV specific IgG antibody (Ab) in serum was measured by the enzyme linked immunosorbent assay (ELISA) in cord blood, consecutive samples taken 3 monthly, and in paired acute and convalescent samples. A linear regression model was used to calculate the rate of RSV-matAb decline. The effect of risk factors on cord blood titres was investigated. Results The half-life of matAb in the Kenyan cohort was calculated to be 79 days (95% confidence limits (CL): 76–81 days). Ninety seven percent of infants were born with RSV-matAb. Infants who subsequently experienced an infection in early life had significantly lower cord titres of anti-RSV Ab in comparison to infants who did not have any incident infection in the first 6 months (P = 0.011). RSV infections were shown to have no effect on the rate of decay of RSV-matAb. Conclusion Maternal-specific RSV Ab decline rapidly following birth. However, we provide evidence of protection against severe disease by RSV-matAb during the first 6–7 months. This suggests that boosting maternal-specific Ab by RSV vaccination may be a useful strategy to consider

Genetic relatedness of infecting and reinfecting respiratory syncytial virus strains identified in a birth cohort from rural Kenya

Background: Respiratory syncytial virus (RSV) reinfects individuals repeatedly. The extent to which this is a consequence of RSV antigenic diversity is unclear. Methods: Six-hundred thirty-five children from rural Kenya were closely monitored for RSV infection from birth through 3 consecutive RSV epidemics. RSV infections were identified by immunofluorescence testing of nasal washing samples collected during acute respiratory illnesses, typed into group A and B, and sequenced in the attachment (G) protein. A positive sample separated from a previous positive by ≥14 days was defined as a reinfection a priori. Results: Phylogenetic analysis was undertaken for 325 (80%) of 409 identified infections, including 53 (64%) of 83 reinfections. Heterologous group reinfections were observed in 28 episodes, and homologous group reinfections were observed in 25 episodes; 10 involved homologous genotypes, 5 showed no amino acid changes, and 3 were separated by 21–24 days and were potentially persistent infections. The temporal distribution of genotypes among reinfections did not differ from that of single infections. Conclusions: The vast majority of infection and reinfection pairs differed by group, genotype, or G amino acid sequence (ie, comprised distinct viruses). The extent to which this is a consequence of immune memory of infection history or prevalent diversity remains unclear

Fire Weather Index application in north-western Italy

International audiencePiedmont region is located in North-Western Italy and is surrounded by the alpine chain and by the Appennines. The region is covered by a wide extension of forests, mainly in its mountain areas (the forests cover 36% of the regional territory). Forested areas are interested by wildfire events. In the period 1997?2005 Piedmont was interested by an average 387 forest fires per year, covering an average 1926 ha of forest per year. Meteorological conditions like long periods without precipitation contribute to create favourable conditions to forest fire development, while the fire propagation is made easier by the foehn winds, frequently interesting the region in winter and spring particularly. The meteorological danger index FWI (Fire Weather Index) was developed by Van Wagner (1987) for the Canadian Forestry Service, providing a complete description of the behaviour of the different forest components in response to the changing weather conditions. We applied the FWI to the Piedmont region on warning areas previously defined for fire management purposes. The meteorological data-set is based on the data of the very-dense non-GTS network of weather stations managed by Arpa Piemonte. The thresholds for the definition of a danger scenarios system were defined comparing historical FWI data with fires occurred on a 5 years period. The implementation of a prognostic FWI prediction system is planned for the early 2008, involving the use of good forecasts of weather parameters at the station locations obtained by the Multimodel SuperEnsemble post-processing technique

Continuous Invasion by Respiratory Viruses Observed in Rural Households During a Respiratory Syncytial Virus Seasonal Outbreak in Coastal Kenya.

BACKGROUND: Households are high-intensity close-contact environments favorable for transmission of respiratory viruses, yet little is known for low-income settings. METHODS: Active surveillance was completed on 47 households in rural coastal Kenya over 6 months during a respiratory syncytial virus (RSV) season. Nasopharyngeal swabs (NPSs) were taken from 483 household members twice weekly irrespective of symptoms. Using molecular diagnostics, NPSs from 6 households were screened for 15 respiratory viruses and the remainder of households only for the most frequent viruses observed: rhinovirus (RV), human coronavirus (HCoV; comprising strains 229E, OC43, and NL63), adenovirus (AdV), and RSV (A and B). RESULTS: Of 16928 NPSs tested for the common viruses, 4259 (25.2%) were positive for ≥1 target; 596 (13.8%) had coinfections. Detection frequencies were 10.5% RV (1780), 7.5% HCoV (1274), 7.3% AdV (1232), and 3.2% RSV (537). On average, each household and individual had 6 and 3 different viruses detected over the study period, respectively. Rhinovirus and HCoV were detected in all the 47 households while AdV and RSV were detected in 45 (95.7%) and 40 (85.1%) households, respectively. The individual risk of infection over the 6-month period was 93.4%, 80.1%, 71.6%, 61.5%, and 37.1% for any virus, RV, HCoV, AdV, and RSV, respectively. NPSs collected during symptomatic days and from younger age groups had higher prevalence of virus detection relative to respective counterparts. RSV was underrepresented in households relative to hospital admission data. CONCLUSIONS: In this household setting, respiratory virus infections and associated illness are ubiquitous. Future studies should address the health and economic implications of these observations

Strength of Coronal Mass Ejection-Driven Shocks Near the Sun, and Its Importance in Predicting Solar Energetic Particle Events

Coronal shocks are important structures, but there are no direct observations of them in solar and space physics. The strength of shocks plays a key role in shock-related phenomena, such as radio bursts and solar energetic particle (SEP) generation. This paper presents an improved method of calculating Alfven speed and shock strength near the Sun. This method is based on using as many observations as possible, rather than one-dimensional global models. Two events, a relatively slow CME on 2001 September 15 and a very fast CME on 2000 June 15, are selected to illustrate the calculation process. The calculation results suggest that the slow CME drove a strong shock, with Mach number of 3.43 - 4.18, while the fast CME drove a relatively weak shock, with Mach number of 1.90 - 3.21. This is consistent with the radio observations, which find a stronger and longer decameter-hectometric (DH) type II radio burst during the first event, and a short DH type II radio burst during the second event. In particular, the alculation results explain the observational fact that the slow CME produced a major solar energetic particle (SEP) event, while the fast CME did not. Through a comparison of the two events, the importance of shock strength in predicting SEP events is addressed

An intensive, active surveillance reveals continuous invasion and high diversity of rhinovirus in households

We report on infection patterns in 5 households (78 participants) delineating the natural history of human rhinovirus (HRV). Nasopharyngeal collections were obtained every 3–4 days irrespective of symptoms, over a 6-month period, with molecular screening for HRV and typing by sequencing VP4/VP2 junction. Overall, 311/3468 (8.9%) collections were HRV positive: 256 were classified into 3 species: 104 (40.6%) HRV-A; 14 (5.5%) HRV-B, and 138 (53.9%) HRV-C. Twenty-six known HRV types (13 HRV-A, 3 HRV-B, and 10 HRV-C) were identified (A75, C1, and C35 being most frequent). We observed continuous invasion and temporal clustering of HRV types in households (range 5–13 over 6 months). Intrahousehold transmission was independent of clinical status but influenced by age. Most (89.0%) of HRV infection episodes were limited to <14 days. Individual repeat infections were frequent (range 1–7 over 6 months), decreasing with age, and almost invariably heterotypic, indicative of lasting type-specific immunity and low cross-type protection

Duration of shedding of respiratory syncytial virus in a community study of Kenyan children

Background: Our understanding of the transmission dynamics of respiratory syncytial virus (RSV) infection will be better informed with improved data on the patterns of shedding in cases not limited only to hospital admissions. Methods: In a household study, children testing RSV positive by direct immunofluorescent antibody test (DFA) were enrolled. Nasal washings were scheduled right away, then every three days until day 14, every 7 days until day 28 and every 2 weeks until a maximum of 16 weeks, or until the first DFA negative RSV specimen. The relationship between host factors, illness severity and viral shedding was investigated using Cox regression methods. Results: From 151 families a total of 193 children were enrolled with a median age of 21 months (range 1-164 months), 10% infants and 46% male. The rate of recovery from infection was 0.22/person/day (95% CI 0.19-0.25) equivalent to a mean duration of shedding of 4.5 days (95%CI 4.0-5.3), with a median duration of shedding of 4 days (IQR 2-6, range 1-14). Children with a history of RSV infection had a 40% increased rate of recovery i.e. shorter duration of viral shedding (hazard ratio 1.4, 95% CI 1.01-1.86). The rate of cessation of shedding did not differ significantly between males and females, by severity of infection or by age. Conclusion: We provide evidence of a relationship between the duration of shedding and history of infection, which may have a bearing on the relative role of primary versus re-infections in RSV transmission in the community

Estudio de factores de riesgo asociados a la infección por Mycoplasma suis

Este estudio se propuso estimar la distribución de la infección por Mycoplasma suis en poblaciones de cerdos de Argentina e identifi car factores de riesgo asociados. Se recolectaron 284 muestras de sangre de cerdos de diferentes categorías productivas en frigorífi cos y granjas de las provincias de Santa Fe, Córdoba y Buenos Aires. Amplifi cando el gen del ARNr 16S de M. suis a través de la reacción en cadena de la polimerasa (PCR), se calculó un porcentaje de infectados del 64%. Se estimó además que no existía asociación estadísticamente signifi cativa (p>0,1) entre un resultado positivo a la PCR y el sexo del animal muestreado, los antecedentes de anemia en la granja y las condiciones de alojamiento. Contrariamente se encontró asociación signifi cativa (p<0,1) con el origen geográfi co y la categoría productiva. Se estimó que los cerdos de Buenos Aires y Córdoba tenían más probabilidades de ser PCR positivos que los de Santa Fe, mientras que los lechones y los cerdos de recría tenían menos riesgo de infectarse que los animales de más edad. Se concluye que el M. suis está ampliamente distribuido en las poblaciones porcinas estudiadas del país.Fil: Pereyra, N. B.. Universidad Nacional de Rosario; ArgentinaFil: Pérez, A. M.. Universidad Nacional de Rosario; ArgentinaFil: Messick, J. B.. Purdue University; Estados UnidosFil: Cane, F. D.. Ministerio de la Producción de la Provincia de Santa Fe; ArgentinaFil: Guglielmone, Alberto Alejandro. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentin

Risk factors and outcomes for the Q151M and T69 insertion HIV-1 resistance mutations in historic UK data

BACKGROUND: The prevalence of HIV-1 resistance to antiretroviral therapies (ART) has declined in high-income countries over recent years, but drug resistance remains a substantial concern in many low and middle-income countries. The Q151M and T69 insertion (T69i) resistance mutations in the viral reverse transcriptase gene can reduce susceptibility to all nucleoside/tide analogue reverse transcriptase inhibitors, motivating the present study to investigate the risk factors and outcomes associated with these mutations. METHODS: We considered all data in the UK HIV Drug Resistance Database for blood samples obtained in the period 1997-2014. Where available, treatment history and patient outcomes were obtained through linkage to the UK Collaborative HIV Cohort study. A matched case-control approach was used to assess risk factors associated with the appearance of each of the mutations in ART-experienced patients, and survival analysis was used to investigate factors associated with viral suppression. A further analysis using matched controls was performed to investigate the impact of each mutation on survival. RESULTS: A total of 180 patients with Q151M mutation and 85 with T69i mutation were identified, almost entirely from before 2006. Occurrence of both the Q151M and T69i mutations was strongly associated with cumulative period of virological failure while on ART, and for Q151M there was a particular positive association with use of stavudine and negative association with use of boosted-protease inhibitors. Subsequent viral suppression was negatively associated with viral load at sequencing for both mutations, and for Q151M we found a negative association with didanosine use but a positive association with boosted-protease inhibitor use. The results obtained in these analyses were also consistent with potentially large associations with other drugs. Analyses were inconclusive regarding associations between the mutations and mortality, but mortality was high for patients with low CD4 at detection. CONCLUSIONS: The Q151M and T69i resistance mutations are now very rare in the UK. Our results suggest that good outcomes are possible for people with these mutations. However, in this historic sample, viral load and CD4 at detection were important factors in determining prognosis